A kind of preparation method of chlorella cell surface oxygen consumption protection layer

A cell surface, chlorella technology, applied in the biological field, to achieve the effect of promoting continuous hydrogen production, good biocompatibility, and maintaining its own activity

Active Publication Date: 2021-06-15
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still relatively few related studies on changing the green algae cells from oxygen production to hydrogen production in the atmospheric environment through cell surface engineering technology.

Method used

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  • A kind of preparation method of chlorella cell surface oxygen consumption protection layer
  • A kind of preparation method of chlorella cell surface oxygen consumption protection layer
  • A kind of preparation method of chlorella cell surface oxygen consumption protection layer

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specific Embodiment approach 1

[0016] Specific embodiment one: the preparation method of a kind of chlorella cell surface oxygen-consuming protective layer of this embodiment is specifically carried out according to the following steps:

[0017] 1. Cultivation of Chlorella pyrenoidosa cells: Select Chlorella pyrenoidosa as a biological template, and use TAP medium to cultivate in a light incubator with a temperature of 25-30°C under continuous light of 1200-4800LUX. When pyrenoid pellets Coating is carried out when the number of algae cells reaches the logarithmic growth phase to obtain Chlorella pyrenoidosa cells in the logarithmic growth phase;

[0018] 2. First use a NaCl solution with a concentration of 0.01 to 0.06mol / L to clean the logarithmic phase of the growth of Chlorella pyrenoidosa cells, and then use deionized water to clean the growth of the logarithmic phase of the Chlorella pyrenoidosa cells, Collect the cells by centrifugation, add the collected cells to the Tris buffer solution to obtain t...

specific Embodiment approach 2

[0022] Embodiment 2: This embodiment differs from Embodiment 1 in that: the OD value of the cell culture buffer in Step 2 is 2.0. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0023] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that the mass ratio of dopamine in step 2 to the cell culture buffer is 1 mg:0.375 mL. Others are the same as in the first or second embodiment.

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Abstract

A method for preparing an oxygen-consuming protective layer on the surface of chlorella cells. The invention belongs to the field of biotechnology, and in particular relates to a method for preparing an oxygen-consuming protective layer on the surface of chlorella cells. The purpose of the present invention is to introduce cell surface engineering technology to the surface of green algae cells, realize the production of hydrogen by green algae cells in the atmospheric environment through the single coating of the surface of green algae cells; artificially build a functional protective layer on the cell surface to improve biological Inherent properties and functions of the body. Methods: 1. Cultivation of chlorella pyrenoidosa cells; 2. Preparation of polydopamine-modified chlorella pyrenoidosa cells; 3. Preparation of chlorella pyrenoidosa cells coated with polydopamine and laccase. The invention constructs a layer of oxygen-consuming protective layer with a two-component structure on the surface of the chlorella pyrenoidosa. The oxygen-consuming protective layer has good biocompatibility, not only can maintain the activity of the organism itself, but also maintain an anaerobic environment around Chlorella pyrenoidosa to generate hydrogen, and has a certain defense protection for the organism when the external environment is unfavorable. effect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing an oxygen-consuming protective layer on the surface of chlorella cells. Background technique [0002] Energy is an important material basis for the survival and development of human society. With the depletion of fossil fuels, seeking and developing clean and non-polluting green alternative energy has become an important issue for scientific and technological workers. As a carbon-free and clean energy, hydrogen energy has the advantages of high calorific value, high energy conversion efficiency and clean and pollution-free combustion products. It is undoubtedly a very potential alternative energy source for fossil fuels. However, hydrogen energy is a secondary energy carrier and must be generated by other energy conversions. At present, the methods of hydrogen production include electrolysis of water to produce hydrogen, water gas method to produc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 黄鑫苏东悦
Owner HARBIN INST OF TECH
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