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Porphyrin nanocomposite with tumor targeting function, preparation method and application thereof

A nanocomposite, tumor targeting technology, applied in the field of porphyrin nanocomposite and its preparation, can solve the problem of low photodynamic therapy efficiency MRI imaging efficiency, etc., achieve excellent in vivo MRI imaging effect, enhanced light absorption ability, enhanced PDT The effect of efficiency

Active Publication Date: 2019-01-25
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the self-assembly method exhibits low photodynamic therapy efficiency and MRI imaging efficiency

Method used

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  • Porphyrin nanocomposite with tumor targeting function, preparation method and application thereof
  • Porphyrin nanocomposite with tumor targeting function, preparation method and application thereof
  • Porphyrin nanocomposite with tumor targeting function, preparation method and application thereof

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preparation example Construction

[0031] The invention provides a method for preparing a porphyrin nanocomposite with tumor targeting function, comprising the following steps:

[0032] (1) Gadolinium acetylacetonate porphyrin, tetraphenyl zinc porphyrin and chloroform are mixed to obtain a porphyrin mixed chloroform solution;

[0033] (2) milking the porphyrin mixed chloroform solution under the action of an emulsifier, removing chloroform to obtain double porphyrin component nanosphere aqueous solution (GZNs);

[0034] (3) extracting the cell membrane fragments of HeLa cells, and sorting to obtain a solution of cell membrane fragments of 200-300nm HeLa cells;

[0035] (4) Mix the cell membrane fragment solution of Hela cells in the step (3) with the double porphyrin component nanosphere aqueous solution, collect the sub-membrane material filtered through a 400nm porous membrane, and obtain the porphyrin nanoparticle wrapped with cell membrane fragments. Complexes (mGZNs);

[0036] There is no time sequence ...

Embodiment 1

[0060] 1) Gadolinium acetylacetonate porphyrin (5 mg) and tetraphenyl zinc porphyrin (5 mg) were mixed in 1 mL of chloroform solvent, and stirred for 30 min to obtain a mixed chloroform solution of the two porphyrins.

[0061] 2) Add the chloroform solution of mixed porphyrins to the aqueous solution of sodium dodecylsulfonate (SDS, 0.01M), ultrasonicate for 1min under the action of ultrasonic emulsification (80W), and then heat in a water bath at 63°C Chloroform was volatilized for 30 minutes, and the volatilized solution was cooled to room temperature to obtain nanocomposite microspheres.

[0062] 3) First culture about 50 million HeLa cells, wash them once with PBS, add cell digestion solution containing EDTA but not trypsin to treat the cells so that the cells no longer adhere to the wall, and blow the cells with a pipette. The cells were collected by centrifugation, the supernatant was sucked off, and the cell pellet was left for later use. The cell pellet was gently resu...

Embodiment 2

[0065] The porphyrin nanocomposite (mGZNs) wrapped cell membrane prepared in Example 1 is carried out transmission electron microscope to observe morphology, the result figure 1 shown.

[0066] Depend on figure 1 As shown in , an obvious coating state can be seen, which proves that the cell membrane has been successfully wrapped on the surface of the nanospheres.

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Abstract

The invention provides a porphyrin nanocomposite with tumor targeting function and a preparation method and application thereof, belonging to the technical field of material chemistry and bio-nano medicine. The invention provides a preparation method of a porphyrin nanocomposite with tumor targeting function, wherein gadolinium acetylacetonate porphyrin and tetraphenylzinc porphyrin are used for driving two hydrophobic porphyrin components to carry out co-assembly by using a co-assembly strategy, and the obtained porphyrin nanocomposite exhibits high photodynamic therapy efficiency and MRI imaging efficiency. Meanwhile, the porphyrin nanocomposites also modified HeLa cell membrane fragments to enhance tumor targeting function and enhance HeLa cell uptake. The porphyrin nanocomposite provided by the invention can be used as an excellent photosensitizer and / or contrast agent.

Description

technical field [0001] The invention belongs to the technical field of material chemistry and bionano medicine, and specifically relates to a porphyrin nanocomposite with tumor targeting function and its preparation method and application. Background technique [0002] Photodynamic therapy (PDT) is a new type of non-invasive tumor treatment. Photosensitizers, light and tissue oxygen are the three major components of PDT. Photosensitizers generate reactive oxygen species (ROS) upon exposure to light, thereby achieving the goal of killing tumor cells. singlet oxygen ( 1 o 2 ) is the key cytotoxic species during PDT treatment. Common PDT photosensitizers include porphyrin derivatives, bodipy dyes, and semiconductor quantum dots. Among them, porphyrin, as an organic conjugated macromolecule, can be derived from rich imaging (MRI, fluorescence, PET and SPET, etc.) and therapeutic (PDT and PTT) functions by changing the type of central metal, which is suitable for a variety o...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K9/51A61K47/46A61K49/08A61K49/18A61P35/00
CPCA61K9/5176A61K41/0076A61K49/08A61K49/1818A61P35/00
Inventor 师冰洋王杰菲王中杰张东亚闫纪亮
Owner HENAN UNIVERSITY
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