A kind of porphyrin nanocomposite with tumor targeting function and its preparation method and application
A nanocomposite, tumor targeting technology, applied in the field of porphyrin nanocomposite and its preparation, can solve the problems of low photodynamic therapy efficiency MRI imaging efficiency, etc., achieve excellent in vivo MRI imaging effect, enhance PDT efficiency, light absorption ability Enhanced effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0031] The invention provides a method for preparing a porphyrin nanocomposite with tumor targeting function, comprising the following steps:
[0032] (1) Gadolinium acetylacetonate porphyrin, tetraphenyl zinc porphyrin and chloroform are mixed to obtain a porphyrin mixed chloroform solution;
[0033] (2) milking the porphyrin mixed chloroform solution under the action of an emulsifier, removing chloroform to obtain double porphyrin component nanosphere aqueous solution (GZNs);
[0034] (3) extracting the cell membrane fragments of HeLa cells, and sorting to obtain a solution of cell membrane fragments of 200-300nm HeLa cells;
[0035] (4) Mix the cell membrane fragment solution of Hela cells in the step (3) with the double porphyrin component nanosphere aqueous solution, collect the sub-membrane material filtered through a 400nm porous membrane, and obtain the porphyrin nanoparticle wrapped with cell membrane fragments. Complexes (mGZNs);
[0036] There is no time sequence ...
Embodiment 1
[0060] 1) Gadolinium acetylacetonate porphyrin (5 mg) and tetraphenyl zinc porphyrin (5 mg) were mixed in 1 mL of chloroform solvent, and stirred for 30 min to obtain a mixed chloroform solution of the two porphyrins.
[0061] 2) Add the chloroform solution of mixed porphyrins to the aqueous solution of sodium dodecylsulfonate (SDS, 0.01M), ultrasonicate for 1min under the action of ultrasonic emulsification (80W), and then heat in a water bath at 63°C Chloroform was volatilized for 30 minutes, and the volatilized solution was cooled to room temperature to obtain nanocomposite microspheres.
[0062] 3) First culture about 50 million HeLa cells, wash them once with PBS, add cell digestion solution containing EDTA but not trypsin to treat the cells so that the cells no longer adhere to the wall, and blow the cells with a pipette. The cells were collected by centrifugation, the supernatant was sucked off, and the cell pellet was left for later use. The cell pellet was gently resu...
Embodiment 2
[0065] The porphyrin nanocomposite (mGZNs) wrapped cell membrane prepared in Example 1 is carried out transmission electron microscope to observe morphology, the result figure 1 shown.
[0066] Depend on figure 1 As shown in , an obvious coating state can be seen, which proves that the cell membrane has been successfully wrapped on the surface of the nanospheres.
PUM
Property | Measurement | Unit |
---|---|---|
pore size | aaaaa | aaaaa |
concentration | aaaaa | aaaaa |
diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com