Multifunctional dura mater repairing material based on biomimetic design and preparation method thereof

Abstract

The invention discloses a multifunctional dura mater repairing material based on biomimetic design and a preparation method thereof. The dura mater repairing material comprises a polylactic acid layerwith hydrophobic and leakproof properties, a hydrogel layer with hydrophilic and repairing functions, and a chitosan with antibacterial, joining and anti-adhesion properties. Polylactide layer. By electrospinning polylactic acid film, electrospinning chitosan- Poly (lactic acid) (PLA) films, gelatin prepared on bilayer electrospun films- SIS pow hydrogel. The dura mater repairing material of theinvention has low cost, and can simultaneously realize the effects of leakproof liquid and induced regeneration, anti-adhesion, anti-immunity and anti-bacterial.

Description

technical field

[0001] The invention belongs to the technical field of tissue engineering of biomedical materials, and in particular relates to a multifunctional dura mater repair material based on bionic design and a preparation method thereof. Background technique

[0002] The dura mater is a thick, tough double membrane. The outer layer is the periosteum on the inner surface of the skull, which is only loosely attached to the calvaria, especially in the occipital and temporal parts, and is called the periosteum layer. However, the sutures of the skull and the base of the skull are more firmly attached and difficult to separate. There is no intracranial intradural space. The inner layer of the dura mater is thicker and tougher than the outer layer, and it is continuous with the dura mater at the foramen magnum, called the meningeal layer. The main function is to protect the brain.

[0003] In daily life, dural defect is a clinical problem often encountered in spinal su...

Examples

Embodiment 1

[0039] A multifunctional dura mater repair material based on bionic design and a preparation method thereof, comprising the following steps:

[0040] 1) Electrospun polylactic acid film

[0041] Inject 1ml of 8.5% (W / V) PLLA (polylactic acid) stirred magnetically for 24 hours into a glass syringe (D=12mm). The molecular weight of polylactic acid in the polylactic acid solution is 286,000, and the solvent is dichloromethane. No. needle, set the distance between the positive and negative electrodes to 15cm, the positive voltage to 10V, the negative voltage to -5V, and the propulsion rate to 0.5ml / h for electrospinning to form a polylactic acid electrospinning film with a micron thickness and a diameter of about 1 micron.

[0042] 2) Electrospun chitosan-polylactic acid film

[0043] A solution of chitosan and polylactic acid containing 2.5% w / v total amount was magnetically stirred for 24 hours, wherein the mass ratio of chitosan and polylactic acid was 8:2, and the solvent was...

Embodiment 2

[0048] A multifunctional dura mater repair material based on bionic design and a preparation method thereof, comprising the following steps:

[0049] 1) Electrospun polylactic acid film

[0050] Inject 1ml of 6% (W / V) PLLA (polylactic acid) stirred magnetically for 24 hours into a glass syringe (D=12mm). The molecular weight of polylactic acid in the polylactic acid solution is 286,000, and the solvent is dichloromethane. No. needle, set the distance between the positive and negative electrodes to 15cm, the positive voltage to 10V, the negative voltage to -5V, and the propulsion rate to 0.5ml / h for electrospinning to form a polylactic acid electrospinning film with a micron thickness and a diameter of about 1 micron.

[0051] 2) Electrospun chitosan-polylactic acid film

[0052] A solution of chitosan and polylactic acid containing 2.5% w / v total amount was magnetically stirred for 24 hours, wherein the mass ratio of chitosan and polylactic acid was 8:2, and the solvent was h...

Embodiment 3

[0058] Such as figure 1 As shown, the surface of the hydrogel layer structure is very smooth, which is the general surface morphology of the hydrogel after drying. The hydrogel layer at the junction of the hydrogel-double-layer spinning membrane is located under the spinning, showing uneven Morphology; moreover, chitosan-PLA spun-embedded within the hydrogel layer and connected the hydrogel layers. Among them, in the interface between the hydrogel and the spinning, the chitosan-polylactic acid spinning wrapped in the polylactic acid spinning is closely combined with the hydrogel, and drives the polylactic acid to combine with the hydrogel, so that the surface of the polylactic acid wraps a layer of hydrogel.

[0059] Human cerebrospinal fluid was prepared in vitro according to the formula of cerebrospinal fluid, and the contact angle experiment was carried out on the electrospun surface of polylactic acid in the material. The results show that the average value of the contac...

technical field

[0001] The invention belongs to the technical field of tissue engineering of biomedical materials, and in particular relates to a multifunctional dura mater repair material based on bionic design and a preparation method thereof. Background technique

[0002] The dura mater is a thick, tough double membrane. The outer layer is the periosteum on the inner surface of the skull, which is only loosely attached to the calvaria, especially in the occipital and temporal parts, and is called the periosteum layer. However, the sutures of the skull and the base of the skull are more firmly attached and difficult to separate. There is no intracranial intradural space. The inner layer of the dura mater is thicker and tougher than the outer layer, and it is continuous with the dura mater at the foramen magnum, called the meningeal layer. The main function is to protect the brain.

[0003] In daily life, dural defect is a clinical problem often encountered in spinal su...

Examples

Embodiment 1

[0039] A multifunctional dura mater repair material based on bionic design and a preparation method thereof, comprising the following steps:

[0040] 1) Electrospun polylactic acid film

[0041] Inject 1ml of 8.5% (W / V) PLLA (polylactic acid) stirred magnetically for 24 hours into a glass syringe (D=12mm). The molecular weight of polylactic acid in the polylactic acid solution is 286,000, and the solvent is dichloromethane. No. needle, set the distance between the positive and negative electrodes to 15cm, the positive voltage to 10V, the negative voltage to -5V, and the propulsion rate to 0.5ml / h for electrospinning to form a polylactic acid electrospinning film with a micron thickness and a diameter of about 1 micron.

[0042] 2) Electrospun chitosan-polylactic acid film

[0043] A solution of chitosan and polylactic acid containing 2.5% w / v total amount was magnetically stirred for 24 hours, wherein the mass ratio of chitosan and polylactic acid was 8:2, and the solvent was...

Embodiment 2

[0048] A multifunctional dura mater repair material based on bionic design and a preparation method thereof, comprising the following steps:

[0049] 1) Electrospun polylactic acid film

[0050] Inject 1ml of 6% (W / V) PLLA (polylactic acid) stirred magnetically for 24 hours into a glass syringe (D=12mm). The molecular weight of polylactic acid in the polylactic acid solution is 286,000, and the solvent is dichloromethane. No. needle, set the distance between the positive and negative electrodes to 15cm, the positive voltage to 10V, the negative voltage to -5V, and the propulsion rate to 0.5ml / h for electrospinning to form a polylactic acid electrospinning film with a micron thickness and a diameter of about 1 micron.

[0051] 2) Electrospun chitosan-polylactic acid film

[0052] A solution of chitosan and polylactic acid containing 2.5% w / v total amount was magnetically stirred for 24 hours, wherein the mass ratio of chitosan and polylactic acid was 8:2, and the solvent was h...

Embodiment 3

[0058] Such as figure 1 As shown, the surface of the hydrogel layer structure is very smooth, which is the general surface morphology of the hydrogel after drying. The hydrogel layer at the junction of the hydrogel-double-layer spinning membrane is located under the spinning, showing uneven Morphology; moreover, chitosan-PLA spun-embedded within the hydrogel layer and connected the hydrogel layers. Among them, in the interface between the hydrogel and the spinning, the chitosan-polylactic acid spinning wrapped in the polylactic acid spinning is closely combined with the hydrogel, and drives the polylactic acid to combine with the hydrogel, so that the surface of the polylactic acid wraps a layer of hydrogel.

[0059] Human cerebrospinal fluid was prepared in vitro according to the formula of cerebrospinal fluid, and the contact angle experiment was carried out on the electrospun surface of polylactic acid in the material. The results show that the average value of the contac...

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