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Preparation method of heparin sodium

A technology of heparin sodium and pig small intestine, applied in the field of biochemical industry, can solve the problem of not being able to release heparin sodium, and achieve the effect of solving the problem of poisoning and high product purity

Inactive Publication Date: 2019-01-29
山阳县恒桓生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The technical problem to be solved by the present invention is to provide a preparation method of heparin sodium, which can solve the problem that simple salt hydrolysis and simple enzymatic hydrolysis cannot completely release heparin sodium from intestinal mucosal cells. The combined process of dissociation completely dissociates heparin from intestinal mucosal cells, thereby completely extracting

Method used

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  • Preparation method of heparin sodium
  • Preparation method of heparin sodium

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Embodiment 1

[0026] The purpose of the present invention is to solve the problem that simple salt hydrolysis and simple enzymatic hydrolysis cannot completely release heparin sodium from intestinal mucosal cells, that is, the combined process of salt hydrolysis and enzymatic hydrolysis can completely dissociate heparin from intestinal mucosal cells, thereby completely Extraction problem. And found more objective production parameters for industrial scale production.

[0027] Concrete steps of the present invention are as follows:

[0028] Autoclave all equipment, reagents, and consumables before the test;

[0029] 1) Dissociation: According to the weight ratio of water: pig small intestine mucosa is 4~5: 1, add water to the pig small intestine mucosa, and add salt to make the liquid salinity 5 degrees, add NaOH to adjust the pH value to 9, stir and heat up to 55°C, after 30 minutes of heat preservation, add 0.5g of alkaline protease 2709 per kilogram of porcine small intestinal mucosa to...

Embodiment 2

[0041] (1), in a 2000-liter reaction tank, pump 400 pairs of pig small intestinal mucosa and water to occupy 90% of the space, add 120 kg of salt, add alkali to adjust the pH value to 9, heat up to 55 ° C, and keep warm for 30 minutes. Alkaline protease (type 2709) 1000 grams, after 2 hours of heat preservation, continue to heat up to 85 ℃, filter and cool to about 55 ℃.

[0042] (2) Add 8000 grams of basic resin Type 98 to the liquid at 55° C., stir and absorb for 8 hours, and filter to wash the resin.

[0043] (3) Add the same amount of 15°C saline as the resin, and perform elution twice, each time for 1 hour, and mix the above-mentioned elution water together.

[0044] (4) Add industrial alcohol to the upper eluent to make the alcohol concentration 45 degrees, let it stand for 24 hours, filter the lower sediment with polyester cloth, and dry it in a hot water bath at 65-75 ° C to obtain a purity of 90 The above activity units / mg heparin sodium product.

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Abstract

The invention relates to a preparation method of heparin sodium, including steps of: 1) dissociation: according to weight ratio of water to swine small intestinal mucosa of 4-5:1, adding water to theswine small intestinal mucosa, adding salt until salinity of the solution is 5 degrees, adding alkali to regulate pH to 9, stirring and heating the solution to 55 DEG C, maintaining the temperature for 30 min, adding alkaline protease 2709 to a reaction tank according to the weight ratio of the swine small intestinal mucosa to the alkaline protease 2709 of 2000-3000:1, maintaining the temperaturefor 2 h, heating the liquid to 85 DEG C and performing filtration; 2) performing adsorption, elution, settlement and filtration, and drying to the filtrate in the step 1) to obtain the heparin sodium.Through combination of salt dissociation and enzymolysis, the heparin is completely dissociated from the intestinal mucosa cells, thus completely extracting the heparin. On the basis of technologiesin the prior art, the unit production value is increased by about 1 / 3 whereas the increased cost is only less than 1% of the part of the increased production value.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a preparation method of heparin sodium. Background technique [0002] Heparin sodium is the sodium salt form of heparin. Heparin is widely present in animal body fluids and tissues. In animals, heparin generally exists in combination with proteins to form protein-polysaccharide complexes. The vast majority of heparin used in the market is extracted from the small intestinal mucosa and lungs of pigs and cattle. The structural formula of heparin is generally considered as follows: [0003] [0004] We have seen that heparin is a kind of heparin compound composed of multiple tetrasaccharide units. The molecular weight of highly active heparin extracted from pig intestinal mucosa and lung is generally between 3000-30000. [0005] Heparin has a wide range of uses, mainly for anticoagulant, antithrombotic, anti-cell aging, anti-tumor metastasis, anti-virus, and an...

Claims

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Application Information

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IPC IPC(8): C08B37/10
CPCC08B37/0075
Inventor 鲁保存
Owner 山阳县恒桓生物科技有限公司
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