Reactor prepared in mode of simultaneously fixing chloroperoxidase to inner and outer walls of surface modified magnetic halloysite nanotubes and application

Abstract

The invention discloses a reactor prepared in a mode of simultaneously fixing chloroperoxidase to the inner and outer walls of surface modified magnetic halloysite nanotubes and application. A methodcomprises the steps that magnetic materials, namely ferroferric oxide, are modified on the halloysite nanotubes firstly; then 3-aminopropyltriethoxysilane and polyethyleneimine are sequentially modified on the surfaces of the magnetic halloysite nanotubes, so that the inner and outer walls of the modified magnetic halloysite nanotubes are both positively charged; and finally, the chloroperoxidaseis fixed to the interiors of the tubes through physical absorption and fixed to the outer walls of the tubes through covalent binding, and the enzyme reactor is obtained. On the basis of simultaneousfixing of the inner and outer walls, the immobilization amount of the chloroperoxidase is increased, the obtained enzyme reactor is easy, convenient and fast to recycle, 92.20% of catalytic activity can be kept after reuse for nine times, heat stability is better than heat stability of free enzymes at 70-80 DEG C, toleration is good in organic solvents such as DMF, methanol and acetonitrile, the degradation efficiency for degrading rifaximin is high, and when the content of the rifaximin is within 50 [mu]g / mL, the degradation efficiency can reach 90% or above.

Description

technical field [0001] The invention belongs to the technical field of enzyme immobilization, in particular to an amino-modified externally modified magnetic halloysite nanotube immobilized chloroperoxidase reactor and its application. Background technique [0002] Biological enzyme is a catalytic organic substance produced by living cells, most of which are proteins. It is a non-toxic and environmentally friendly biocatalyst. Biological enzymes have very high catalytic efficiency and high specificity. An enzyme can only catalyze one or one type of chemical reaction, and the reaction conditions are mild. However, free enzymes are easily inactivated in environments such as high temperature, strong acid and strong alkali, and organic solvents. The use of immobilized enzymes can effectively improve the tolerance of enzymes to temperature, acidity, alkali, and organic solvents. Modification of the immobilized carrier with magnetic substances makes the recovery of the enzyme eas...

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem of low enzyme immobilization capacity in the existing halloysite nanotube immobilized enzyme technology, and utilize the advantages of different surface components of the halloysite nanotube to first carry out surface modification on its outer surface, It not only introduces magnetic materials to improve its reusability, but also introduces multiplied amino groups to improve the immobilization effect, and can also change the surface electrical properties of halloysite nanotubes, so that the inner and outer walls of the halloysite nanotubes have positive charges at the same time; on this basis, by reducing Vacuum operation and change of pH to adjust the charge of CPO, thus providing a magnetic halloysite nanotube inner and outer walls while immobilizing the chloroperoxidase reactor

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