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Surface-modified magnetic halloysite nanotube inner and outer walls simultaneously immobilized chloroperoxidase reactor and its application

A halloysite nanotube, chloroperoxidase technology, applied in the directions of oxidoreductase, immobilized on or in an inorganic carrier, enzyme, etc., can solve the problem of changing the surface electrical properties of halloysite nanotubes, enzyme immobilization The problem of low load is achieved, and the stability and operation stability are improved, objective economic benefits, and the effect of increasing the fixed load

Active Publication Date: 2021-10-29
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem of low enzyme immobilization capacity in the existing halloysite nanotube immobilized enzyme technology, and utilize the advantages of different surface components of the halloysite nanotube to first carry out surface modification on its outer surface, It not only introduces magnetic materials to improve its reusability, but also introduces multiplied amino groups to improve the immobilization effect, and can also change the surface electrical properties of halloysite nanotubes, so that the inner and outer walls of the halloysite nanotubes have positive charges at the same time; on this basis, by reducing Vacuum operation and change of pH to adjust the charge of CPO, thus providing a magnetic halloysite nanotube inner and outer walls while immobilizing the chloroperoxidase reactor

Method used

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  • Surface-modified magnetic halloysite nanotube inner and outer walls simultaneously immobilized chloroperoxidase reactor and its application
  • Surface-modified magnetic halloysite nanotube inner and outer walls simultaneously immobilized chloroperoxidase reactor and its application
  • Surface-modified magnetic halloysite nanotube inner and outer walls simultaneously immobilized chloroperoxidase reactor and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0022] 1. Preparation of magnetic halloysite nanotubes

[0023] Weigh 5g of halloysite nanotubes into a 600mL beaker, add 400mL of deionized water, sonicate for 15min, continue to add 3.8g of FeCl 3 ·6H 2 O and 3g FeSO 4 ·7H 2 O, after completely dissolving, transfer to a three-neck flask, add 3 to 5 drops of concentrated hydrochloric acid, and pass through N 2 , After 3 hours in an oil bath at 120°C, stop heating and cool to 50°C. Then add 4moL·L dropwise -1 NaOH aqueous solution, adjust the pH value to 9-10, continue to stir at 50°C for 2.5h, obtain a black solid and collect it with a strong magnet, alternately centrifuge and wash with deionized water and absolute ethanol until the supernatant is colorless, and vacuum dry at 50°C for 6h , to obtain magnetic halloysite nanotubes.

[0024] 2. Preparation of surface-modified magnetic halloysite nanotubes

[0025] Weigh 2g of magnetic halloysite nanotubes into a 250mL beaker, add 100mL of absolute ethanol, and stir at a s...

Embodiment 2

[0055] Adopt the CPO@HNTs-Fe that embodiment 1 prepares 3 o 4 -APTES-PEI degradation of rifaximin

[0056] 1. Preparation of mobile phase

[0057] Weigh KH 2 PO 4 3.4g, dissolve and make up to volume in a 250mL volumetric flask, weigh 2.05g of NaAc, dissolve and make up to volume in a 250mL volumetric flask, then mix the two in equal volumes. Use 0.1mol·L -1 Aqueous citric acid solution is used to adjust the pH of the solution to 2.6, and this solution is a buffer solution. Acetonitrile, methanol, buffer volume ratio = 44:16:40 as the mobile phase.

[0058] 2. Degradation of rifaximin

[0059] Using distilled water as solvent, prepare samples of rifaximin standard solution (10 μg / mL, 20 μg / mL, 30 μg / mL, 30 μg / mL, 40 μg / mL, 50 μg / mL) with different concentrations; take five 10 mL centrifuge tubes respectively Add 500 μL rifaximin standard solution, 2400 μL PBS buffer solution (pH=2.75), 10 mg CPO@HNTs-Fe 3 o 4 -APTES-PEI, 100 μL of 30% H 2 o 2 Solution, react at room...

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Abstract

The invention discloses a surface-modified magnetic halloysite nanotube inner and outer walls simultaneously immobilizing a chloroperoxidase reactor and its application. The method firstly modifies the magnetic material ferroferric oxide on the halloysite nanotube, and then The surface of the halloysite nanotubes is sequentially modified with 3-aminopropyltriethoxysilane and polyethyleneimine, so that the inner and outer walls of the modified magnetic halloysite nanotubes are positively charged, and finally in the tube through physical adsorption, outer wall By immobilizing chloroperoxidase through covalent bonding, an enzyme reactor is obtained. The present invention increases the immobilization capacity of chloroperoxidase based on simultaneous immobilization of the inner and outer walls, and the obtained enzyme reactor is simple and convenient to recycle, and can maintain 92.20% of the catalytic activity after repeated use for 9 times, and has a higher thermal stability at 70-80°C than free Good enzyme, good tolerance in DMF, methanol, acetonitrile and other organic solvents, high degradation efficiency for degrading rifaximin, when the content of rifaximin is within 50 μg / mL, the degradation efficiency can reach 90 %above.

Description

technical field [0001] The invention belongs to the technical field of enzyme immobilization, in particular to an amino-modified externally modified magnetic halloysite nanotube immobilized chloroperoxidase reactor and its application. Background technique [0002] Biological enzyme is a catalytic organic substance produced by living cells, most of which are proteins. It is a non-toxic and environmentally friendly biocatalyst. Biological enzymes have very high catalytic efficiency and high specificity. An enzyme can only catalyze one or one type of chemical reaction, and the reaction conditions are mild. However, free enzymes are easily inactivated in environments such as high temperature, strong acid and strong alkali, and organic solvents. The use of immobilized enzymes can effectively improve the tolerance of enzymes to temperature, acidity, alkali, and organic solvents. Modification of the immobilized carrier with magnetic substances makes the recovery of the enzyme eas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14A23L5/20
CPCA23L5/25C12N9/0065C12N11/14C12Y111/0101
Inventor 蒋育澄陈宝林胡满成李淑妮翟全国
Owner SHAANXI NORMAL UNIV
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