Preparation method and application of fermented Chinese herbal medicine biological feed for ruminants
A technology for fermenting Chinese herbal medicines and biological feeds, applied in animal feeds, animal feeds, applications, etc., can solve the problem that the species and quantity cannot be accurately controlled with the feeding environment and rumen system, prevention and control There are no reports in this regard, the active ingredients of Chinese herbal medicines Dissolution and other problems to achieve the effect of enhancing the body's adaptability, reducing the use of drugs, and reducing the cost of feeding
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Embodiment 1
[0071] A preparation method of fermented Chinese herbal medicine biological feed for ruminants, comprising the following steps:
[0072] Step (1): respectively inoculate Aspergillus oryzae, Bacillus natto, Phaffia rhodozyma, Enterococcus faecium and Pediococcus pentosaceus in the sterilized separate culture medium, and after the culture is completed, separate rice Aspergillus, Bacillus natto, Phaffia rhodozyma, Enterococcus faecium and Pediococcus pentosaceus;
[0073] The Aspergillus oryzae and Bacillus natto culture mediums are LB culture medium, the Phaffia rhodozyma culture medium is PDA culture medium, and the Enterococcus faecium and Pediococcus pentosaceus culture mediums are MRS culture medium.
[0074] The culture temperature of the Aspergillus oryzae, Bacillus natto, Enterococcus faecium and Pediococcus pentosaceus is 37°C, and the culture temperature of Phaffia rhodozyma is 32°C;
[0075] The cultivation time of described Aspergillus oryzae, Bacillus natto, Enteroc...
Embodiment 2
[0092] A preparation method of fermented Chinese herbal medicine biological feed for ruminants, comprising the following steps:
[0093] Step (1): respectively inoculate Aspergillus oryzae, Bacillus natto, Phaffia rhodozyma, Enterococcus faecium and Pediococcus pentosaceus in the sterilized separate culture medium, and after the culture is completed, separate rice Aspergillus, Bacillus natto, Phaffia rhodozyma, Enterococcus faecium and Pediococcus pentosaceus;
[0094] The Aspergillus oryzae and Bacillus natto culture mediums are both LB culture medium, the Phaffia rhodozyma culture medium is PDA culture medium, and the Enterococcus faecium and Pediococcus pentosaceus culture mediums are MRS culture medium.
[0095] The culture temperature of the Aspergillus oryzae, Bacillus natto, Enterococcus faecium and Pediococcus pentosaceus is 37°C, and the culture temperature of Phaffia rhodozyma is 25°C;
[0096] The cultivation time of described Aspergillus oryzae, Bacillus natto, En...
Embodiment 3
[0113] A preparation method of fermented Chinese herbal medicine biological feed for ruminants, comprising the following steps:
[0114] Step (1): respectively inoculate Aspergillus oryzae, Bacillus natto, Phaffia rhodozyma, Enterococcus faecium and Pediococcus pentosaceus in the sterilized separate culture medium, and after the culture is completed, separate rice Aspergillus, Bacillus natto, Phaffia rhodozyma, Enterococcus faecium and Pediococcus pentosaceus;
[0115] The Aspergillus oryzae and Bacillus natto culture mediums are LB culture medium, the Phaffia rhodozyma culture medium is PDA culture medium, and the Enterococcus faecium and Pediococcus pentosaceus culture mediums are MRS culture medium.
[0116] The culture temperature of the Aspergillus oryzae, Bacillus natto, Enterococcus faecium and Pediococcus pentosaceus is 37°C, and the culture temperature of Phaffia rhodozyma is 27°C;
[0117] The cultivation time of described Aspergillus oryzae, Bacillus natto, Enteroc...
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