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Preparation method and application of fermented Chinese herbal medicine biological feed for ruminants

A technology for fermenting Chinese herbal medicines and biological feeds, applied in animal feeds, animal feeds, applications, etc., can solve the problem that the species and quantity cannot be accurately controlled with the feeding environment and rumen system, prevention and control There are no reports in this regard, the active ingredients of Chinese herbal medicines Dissolution and other problems to achieve the effect of enhancing the body's adaptability, reducing the use of drugs, and reducing the cost of feeding

Active Publication Date: 2022-06-14
湖北华大瑞尔科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, in the process of ruminant breeding, some enterprises have added beneficial microorganisms to regulate the rumen environment, which can increase the feed intake of ruminants, and the quality of meat and milk has also been improved to some extent, but there is almost no such aspect in the prevention and control of diseases. According to reports, because beneficial microorganisms are living organisms, they also need to consume a certain amount of energy. Therefore, in some ruminant breeding enterprises, the cost of feeding has not decreased, but has increased to a certain extent. The main reason is that the types and quantities of beneficial microorganisms and the breeding environment and the rumen system cannot be precisely controlled; some breeding companies directly add Chinese herbal medicines to the rumen feed to regulate the rumen system, but the active ingredients of Chinese herbal medicines such as: saponins, sterols, astragalus polysaccharides, etc. are all in the cells of Chinese herbal medicine plants, through direct feeding Or simple pretreatment cannot make the active ingredients of Chinese herbal medicine dissolve quickly from plant cells, and cannot be absorbed and utilized by ruminants to exert their efficacy. Therefore, it exceeds people's expectations that Chinese herbal medicine can prevent and control diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] A preparation method of fermented Chinese herbal medicine biological feed for ruminants, comprising the following steps:

[0072] Step (1): respectively inoculate Aspergillus oryzae, Bacillus natto, Phaffia rhodozyma, Enterococcus faecium and Pediococcus pentosaceus in the sterilized separate culture medium, and after the culture is completed, separate rice Aspergillus, Bacillus natto, Phaffia rhodozyma, Enterococcus faecium and Pediococcus pentosaceus;

[0073] The Aspergillus oryzae and Bacillus natto culture mediums are LB culture medium, the Phaffia rhodozyma culture medium is PDA culture medium, and the Enterococcus faecium and Pediococcus pentosaceus culture mediums are MRS culture medium.

[0074] The culture temperature of the Aspergillus oryzae, Bacillus natto, Enterococcus faecium and Pediococcus pentosaceus is 37°C, and the culture temperature of Phaffia rhodozyma is 32°C;

[0075] The cultivation time of described Aspergillus oryzae, Bacillus natto, Enteroc...

Embodiment 2

[0092] A preparation method of fermented Chinese herbal medicine biological feed for ruminants, comprising the following steps:

[0093] Step (1): respectively inoculate Aspergillus oryzae, Bacillus natto, Phaffia rhodozyma, Enterococcus faecium and Pediococcus pentosaceus in the sterilized separate culture medium, and after the culture is completed, separate rice Aspergillus, Bacillus natto, Phaffia rhodozyma, Enterococcus faecium and Pediococcus pentosaceus;

[0094] The Aspergillus oryzae and Bacillus natto culture mediums are both LB culture medium, the Phaffia rhodozyma culture medium is PDA culture medium, and the Enterococcus faecium and Pediococcus pentosaceus culture mediums are MRS culture medium.

[0095] The culture temperature of the Aspergillus oryzae, Bacillus natto, Enterococcus faecium and Pediococcus pentosaceus is 37°C, and the culture temperature of Phaffia rhodozyma is 25°C;

[0096] The cultivation time of described Aspergillus oryzae, Bacillus natto, En...

Embodiment 3

[0113] A preparation method of fermented Chinese herbal medicine biological feed for ruminants, comprising the following steps:

[0114] Step (1): respectively inoculate Aspergillus oryzae, Bacillus natto, Phaffia rhodozyma, Enterococcus faecium and Pediococcus pentosaceus in the sterilized separate culture medium, and after the culture is completed, separate rice Aspergillus, Bacillus natto, Phaffia rhodozyma, Enterococcus faecium and Pediococcus pentosaceus;

[0115] The Aspergillus oryzae and Bacillus natto culture mediums are LB culture medium, the Phaffia rhodozyma culture medium is PDA culture medium, and the Enterococcus faecium and Pediococcus pentosaceus culture mediums are MRS culture medium.

[0116] The culture temperature of the Aspergillus oryzae, Bacillus natto, Enterococcus faecium and Pediococcus pentosaceus is 37°C, and the culture temperature of Phaffia rhodozyma is 27°C;

[0117] The cultivation time of described Aspergillus oryzae, Bacillus natto, Enteroc...

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PUM

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Abstract

The invention discloses a preparation method and application of a fermented Chinese herbal medicine biological feed for ruminants, wherein the raw material ratio of the Chinese herbal medicine is 21-29 parts of astragalus, 18-32 parts of poria cocos, 22-28 parts of codonopsis pilosula, and 9-18 parts of mugwort , 10-20 parts of licorice, after inoculating Aspergillus oryzae, Bacillus natto and Phaffia rhodozyme for aerobic fermentation, then inoculating Enterococcus faecium and Pediococcus pentosaceae for anaerobic fermentation to prepare fermented Chinese herbal medicine biological feed. The raw materials of the Chinese herbal medicine of the present invention have reasonable compatibility, and through the step-by-step fermentation of beneficial microorganisms, the efficacy of the Chinese herbal medicine can be significantly improved, and a large number of metabolites can be produced at the same time, which can improve the rumen environment of ruminants, improve feed utilization, reduce diseases, reduce feeding costs, and improve economic benefits.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a preparation method and application of a fermented Chinese herbal medicine biological feed for ruminants. Background technique [0002] Rumination is the process of returning half-digested food in the stomach to the mouth to chew again after a period of time. Ruminants are animals that ruminate, usually herbivores, because the fiber of plants is more difficult to digest. Ruminants are generally in a hurry to eat, especially roughage, most of which are swallowed into the rumen without adequate chewing. After soaking and softening in the rumen for a period of time, the food returns to the mouth after revomiting, and after re-chewing, it is mixed with saliva again and then again. The process of swallowing into the rumen. The rumen of ruminants is a unique digestive fermenter inhabited by a variety of microorganisms, including rumen bacteria, anaerobic fungi, and rumen prot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12A23K50/10A61K36/076A61K36/282A61K36/484A61P1/14A61P1/16A61P1/00A61P37/04A61P39/00A61P31/04
CPCA61P1/00A61P1/14A61P1/16A61P31/04A61P37/04A61P39/00A23K10/12A23K50/10A61K36/076A61K36/282A61K36/344A61K36/481A61K36/484A61K2236/19A61K2300/00
Inventor 李钢平李筱雯李元平韩继宏崔卫涛况世昌欧阳潮龙兴权姜涛
Owner 湖北华大瑞尔科技有限公司
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