Oleralignan in portulaca oleracea as well as extraction and separation method and applications thereof
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A separation method and technology of purslane, which are applied in the directions of ether separation/purification, antidote, ether preparation, etc., can solve the problem of low structural novelty, and achieve the effects of environmental protection process method, simple and fast operation method
Active Publication Date: 2019-02-15
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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[0004]
At present, most of the chemical components isolated from purslane are known, and the structural novelty is low. Therefore, the development and isolation of new compounds in purslane are urgently needed
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Embodiment 1
[0030] The present invention provides new compound, molecular formula is C 18 h 16 o 4 , named as olealignan chemical structure shown in Figure 10.
[0031] Described new compound is called olealignan according to structure, and table 1 is the NMR data of this new compound: 1 H-NMR with 13 C-NMR in deuterated DMSO.
[0032]
[0033]
[0034] The structural identification of the compounds of the present invention can be referred to Figure 1-10 .
[0035] Oleralignan: Yellow-green powder, easily soluble in methanol. After spotting the sample on the silica gel thin-layer plate, spray the ferric chloride test solution and the spot turns blue. UV(MeOH)λ max : 291,236nm. IR v max 3393,2925,2850,1600, 1515,1478,1256,1203,1159,1125,1021,859,790,762cm -1 . HRESI(+)TOFMS gives m / z: 297.1092[M+H] + The quasi-molecular ion peak has a molecular weight of 296.1043. combine 1 H-NMR, 13 According to C-NMR and DEPT data, it is speculated that the possible molecular form...
Embodiment 2
[0046] Embodiment 2 is basically the same as Embodiment 1, and its difference is:
[0047] Water is 8 times in step 1.
[0048] Step 6: Acetonitrile: 0.1% (volume percent) formic acid (48:52, v:v) was used as the mobile phase, and the detection wavelength was 210nm, 280nm, and the new compound of the present invention was obtained by separation with a purity of 93%.
Embodiment 3
[0050] Embodiment 3 is basically the same as Embodiment 1, and its difference is:
[0051] Water is 16 times in step 1.
[0052] In step 6, acetonitrile: 0.1% (volume percent) formic acid (50:50, v:v) was used as the mobile phase, and the detection wavelength was 210nm, 280nm to separate and prepare the new compound of the present invention with a purity of 90%.
[0053] Obtained from embodiment 1-3: the purity measured by normalization method is 90~97.5%.
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Abstract
The invention relates to oleralignan in portulaca oleracea as well as an extraction and separation method and applications thereof, in particular relates to a new lignans compound extracted, separatedand identified from the portulaca oleracea as well as the extraction and separation method thereof. The molecular formula of the new compound is C18H16O4, and the new compound is named as oleralignan. The invention further provides the extraction and separation method of the new compound, which is adopted for successfully extracting and separating the new lignans compound by performing the following steps in turn: extraction by boiling with water, silica gel column chromatography, polyamide column chromatography, ODS medium pressure column chromatography and Sephadex LH-20 separation. The structure of the oleralignan is identified to be the new lignans compound by the method of analyzing mass spectroscopy, carbon spectroscopy, hydrogen spectroscopy and two-dimensional nuclear magnetic resonance spectroscopy, and the new lignans compound has anti-tumor and antioxidant effects. The new compound provided by the invention as well as the salts or derivatives thereof can be taken as primersfor synthesizing other compounds and raw materials for developing new medicines and studying pharmacological activity.
Description
technical field [0001] The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to new compounds extracted, separated and identified from purslane medicinal materials and their extraction and separation methods, specifically the extraction and separation method of Oleralignan (purslane lignin) in purslane and its application. Background technique [0002] Purslane (Portulaca oleracea L.), also known as amaranth, longevity vegetable, ant vegetable, is an annual herb of the family Portulacaceae. Purslane is widely distributed and rich in resources. It is one of the 78 wild plants with the same origin of medicine and food as stipulated by the Ministry of Health of my country. Purslane is recorded in the 2015 edition of "Pharmacopoeia of the People's Republic of China". It has the functions of clearing heat and detoxifying, cooling blood and stopping bleeding, and stopping dysentery. , hemorrhoids, metrorrhagia, etc. [0...
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