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Method of converting phlorizin into phloretin

A technology of phloridzin and phloretin, applied in the biological field, can solve the problems of difficult industrialized large-scale production, poor conversion specificity, high cost, etc., achieve good dispersion effect and improve the conversion rate

Inactive Publication Date: 2019-02-19
GUILIN NATURAL INGREDIENTS CORP
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  • Abstract
  • Description
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Problems solved by technology

[0005] The application of the current existing technology has certain limitations, such as the use of acid-base hydrolysis, although it has the advantage of low cost, but there are strong acid and strong alkali residues, environmental pollution, poor conversion specificity and decolorization of products; using enzymatic hydrolysis The law requires commercial hydrolytic enzymes, and the enzymes cannot be recycled and reused, resulting in high process costs. At the same time, the hydrolytic enzymes are used as raw materials, and the cost is relatively high, which is not easy for industrialized mass production

Method used

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  • Method of converting phlorizin into phloretin
  • Method of converting phlorizin into phloretin
  • Method of converting phlorizin into phloretin

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Add water into the reaction tank, use hydrochloric acid to adjust the pH value to 3.5, and add the dry powder containing 80% mass fraction of phloridin into the reaction tank according to the ratio of phloridin-containing raw material to water mass ratio of 1:10. Heating to 45°C, adding the mixed enzyme of cellulose compound enzyme and pectin compound enzyme, the mass ratio of mixed enzyme and phloridin raw material is 1:10, and the mass ratio of cellulose compound enzyme and pectin compound enzyme is 1:1 , 45°C for 10 hours for enzymatic hydrolysis; every 0.5 hours, nitrogen gas is continuously introduced from the bottom of the solution for 1 minute to obtain an enzymatic hydrolysis feed solution; the obtained enzymatic hydrolysis feed liquid is heated to 75°C and inactivated for 10 minutes; a cloth bag centrifuge is used Solid-liquid separation is carried out to obtain wet crystals of phloretin, and dry powdered phloretin products are obtained after drying. The conver...

Embodiment 2

[0038] Add a concentration of 0.5% ethanol aqueous solution to the reaction tank, use hydrochloric acid to adjust the pH value to 5.5, and make the dry powder containing 92.5% mass fraction phlorizin according to the ratio of phloridin-containing raw material to ethanol mass ratio of 1:40 Add to reaction tank. Heating to 55°C, adding the mixed enzyme of cellulose compound enzyme and pectin compound enzyme, the mass ratio of mixed enzyme and phloridin raw material is 1:40, and the mass ratio of cellulose compound enzyme and pectin compound enzyme is 1:100 , 55 ℃ heat preservation enzymolysis for 96 hours; every 2 hours from the bottom of the solution for 5 minutes to continuously pass nitrogen gas to obtain the enzymolysis feed solution; heat the obtained enzymolysis feed solution to 100 ℃, inactivate for 45 minutes; use suction filtration irrigation Solid-liquid separation is carried out to obtain wet crystals of phloretin, and dry powdered phloretin products are obtained afte...

Embodiment 3

[0040] Add a concentration of 5% ethanol aqueous solution to the reaction tank, use hydrochloric acid to adjust the pH value to 4.5, and add the dry powder containing 99% mass fraction of phlorizin according to the ratio of phloridin-containing raw material to ethanol mass ratio of 1:25 in the reaction tank. Heat to 52°C, add the mixed enzyme of cellulose compound enzyme and pectin compound enzyme, the mass ratio of mixed enzyme to phloridin raw material is 1:25, the mass ratio of cellulose compound enzyme and pectin compound enzyme is 1:2 , 52 ℃ heat preservation enzymolysis for 20 hours; every 1 hour from the bottom of the solution into the nitrogen for 3 minutes to obtain the enzymolysis feed solution; the obtained enzymolysis feed solution was heated to 80 ℃, inactivated for 30 minutes; plate and frame press The filter is used for solid-liquid separation to obtain wet crystals of phloretin, which are then dried to obtain dry powder phloretin products. The conversion rate ...

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Abstract

The invention provides a method of converting phlorizin into phloretin, comprising the steps of dissolving a raw material containing phlorizin in ethanol or water solution; adding a mixture of cellulase complex and pectinase complex, allowing enzymatic hydrolysis to occur at 45-55 DEG for 19-96 hours, deactivating after enzymatic hydrolysis, and carrying out solid-liquid separation to obtain finished phloretin. The use of the mixture helps solve the problem that a single enzyme experiences incomplete enzymatic hydrolysis; phlorizin conversion rate is increased; phlorizin conversion rate can beincreased to 95% and above. The phloretin converted herein is white; the traditional process steps that, for instance, acid hydrolysis process requires discoloring are omitted, and production cost isfurther reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for converting phlorizin into phloretin. Background technique [0002] Phloretin (phloretin), also known as trihydric phenol acetone, CAS number 60-82-2, belongs to dihydrochalcone compounds. The rare content and low extraction rate of phloretin in natural plants limit the development and application of phloretin. Therefore, the research on the synthesis and purification of phloretin is of great significance to meet the needs of scientific research and market. [0003] At present, the industrialized production of phloretin mainly adopts acid-base hydrolysis or enzymatic hydrolysis to convert phloridin into phloretin, such as: Chinese patent CN103351291B discloses a semi-synthetic process for preparing phloretin from natural phloridin; the invention Put the crude product of phlorizin in a stainless steel reactor, dissolve it in a 5% strong alkaline solution and simply refine...

Claims

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Application Information

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IPC IPC(8): C12P7/26
CPCC12P7/26
Inventor 杨文国杨美英谢永富赵军
Owner GUILIN NATURAL INGREDIENTS CORP
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