Method of converting phlorizin into phloretin

A technology of phloridzin and phloretin, applied in the biological field, can solve the problems of difficult industrialized large-scale production, poor conversion specificity, high cost, etc., achieve good dispersion effect and improve the conversion rate

Inactive Publication Date: 2019-02-19
GUILIN NATURAL INGREDIENTS CORP
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The application of the current existing technology has certain limitations, such as the use of acid-base hydrolysis, although it has the advantage of low cost, but there are strong acid and strong alkali residues, environmental pollution, poor conversion specificity and decolorization of products; u

Method used

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  • Method of converting phlorizin into phloretin
  • Method of converting phlorizin into phloretin
  • Method of converting phlorizin into phloretin

Examples

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Example Embodiment

[0035] Example 1

[0036] Add water to the reaction tank, adjust the PH value to 3.5 with hydrochloric acid, and add the dry powder containing 80% phloridin into the reaction tank at a ratio of 1:10 by mass of the raw material containing phloridin to the water. Heat to 45℃, add the mixed enzyme of cellulose complex enzyme and pectin complex enzyme, the mass ratio of mixed enzyme to phlorizin raw material is 1:10, and the mass ratio of cellulose complex enzyme to pectin complex enzyme is 1:1 Enzymatic hydrolysis at 45°C for 10 hours; nitrogen is continuously injected from the bottom of the solution for 1 minute every 0.5 hours to obtain the enzymatic hydrolysate solution; the obtained enzymatic hydrolysis solution is heated to 75°C and inactivated for 10 minutes; a cloth bag centrifuge is used Solid-liquid separation is performed to obtain wet phloretin crystals, and dry phloretin products are obtained after drying. The conversion rate of phloretin was 91.4%, and the purity of ph...

Example Embodiment

[0037] Example 2

[0038] Add 0.5% ethanol aqueous solution to the reaction tank, use hydrochloric acid to adjust the PH value to 5.5, according to the ratio of the mass ratio of the raw material containing phloridin to the ethanol is 1:40, the dry powder containing the 92.5% mass fraction of phloridin Add to the reaction tank. Heat to 55℃, add the mixed enzyme of cellulose complex enzyme and pectin complex enzyme, the mass ratio of mixed enzyme and phlorizin raw material is 1:40, and the mass ratio of cellulose complex enzyme and pectin complex enzyme is 1:100 Enzymatic hydrolysis at 55°C for 96 hours; nitrogen is continuously injected from the bottom of the solution for 5 minutes every 2 hours to obtain an enzymatic hydrolysate solution; the obtained enzymatic hydrolysis solution is heated to 100°C and inactivated for 45 minutes; suction filtration is used Solid-liquid separation is performed to obtain wet phloretin crystals, and dry phloretin products are obtained after dryin...

Example Embodiment

[0039] Example 3

[0040] Add a 5% ethanol aqueous solution to the reaction tank, use hydrochloric acid to adjust the PH value to 4.5, and add the dry powder containing 99% phloridin in the mass ratio of phlorizin to ethanol at a ratio of 1:25 In the reaction tank. Heat to 52°C, add the mixed enzyme of cellulose complex enzyme and pectin complex enzyme, the mass ratio of mixed enzyme to phlorizin raw material is 1:25, and the mass ratio of cellulose complex enzyme to pectin complex enzyme is 1:2 Incubate at 52°C for 20 hours; continue to pour nitrogen from the bottom of the solution every hour for 3 minutes to obtain the enzymatic hydrolysate solution; heat the obtained enzymatic hydrolysate solution to 80°C and inactivate for 30 minutes; use plate and frame pressure The filter performs solid-liquid separation to obtain wet phloretin crystals, and dry phloretin products are obtained after drying. The conversion rate of phloridin was 96.1%, and the purity of phloridin was 99.1%....

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Abstract

The invention provides a method of converting phlorizin into phloretin, comprising the steps of dissolving a raw material containing phlorizin in ethanol or water solution; adding a mixture of cellulase complex and pectinase complex, allowing enzymatic hydrolysis to occur at 45-55 DEG for 19-96 hours, deactivating after enzymatic hydrolysis, and carrying out solid-liquid separation to obtain finished phloretin. The use of the mixture helps solve the problem that a single enzyme experiences incomplete enzymatic hydrolysis; phlorizin conversion rate is increased; phlorizin conversion rate can beincreased to 95% and above. The phloretin converted herein is white; the traditional process steps that, for instance, acid hydrolysis process requires discoloring are omitted, and production cost isfurther reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for converting phlorizin into phloretin. Background technique [0002] Phloretin (phloretin), also known as trihydric phenol acetone, CAS number 60-82-2, belongs to dihydrochalcone compounds. The rare content and low extraction rate of phloretin in natural plants limit the development and application of phloretin. Therefore, the research on the synthesis and purification of phloretin is of great significance to meet the needs of scientific research and market. [0003] At present, the industrialized production of phloretin mainly adopts acid-base hydrolysis or enzymatic hydrolysis to convert phloridin into phloretin, such as: Chinese patent CN103351291B discloses a semi-synthetic process for preparing phloretin from natural phloridin; the invention Put the crude product of phlorizin in a stainless steel reactor, dissolve it in a 5% strong alkaline solution and simply refine...

Claims

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Application Information

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IPC IPC(8): C12P7/26
CPCC12P7/26
Inventor 杨文国杨美英谢永富赵军
Owner GUILIN NATURAL INGREDIENTS CORP
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