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Extraction method of small molecule liver peptide

An extraction method and small molecule technology, applied in the field of biological peptide separation, can solve the problems of low protein extraction efficiency, low content of small molecular polypeptides, and wide product molecular weight distribution, and achieve the effect of improving storage stability, small molecular weight, and maintaining activity.

Pending Publication Date: 2019-02-22
河北肽都生物科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of low protein extraction efficiency, low content of extracted small molecule polypeptide and wide molecular weight distribution in the existing small molecule hepatic peptide extraction process, the present invention provides a small molecule hepatic peptide extraction method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A kind of extraction method of small molecule bovine liver peptide:

[0038] Step 1. Select 1000g of fresh beef liver, crush it, add 5000ml of purified water and stir evenly, raise the temperature to 54°C, add 8g of lipase and 10g of neutral protease, stir at a speed of 68r / min for 25min to obtain the first enzymolysis liquid;

[0039] Step 2: Add 3g flavored protease, 15g chicken intestine digestive tract protease and 90mg enzyme activator to the first enzymolysis solution, stir evenly, cool down to 15°C, stir enzymolysis at a speed of 68r / min, and repeatedly extract 2 times, each enzymatic hydrolysis for 1.5h, filtered, and each extract was combined to obtain the second enzymatic hydrolysis solution;

[0040]Step 3: Warm up the enzymolysis solution to 90°C, and inactivate the enzyme for 15 minutes; after inactivating the enzyme, use a decanter centrifuge to filter and remove the enzymolysis residue, and the speed of the decanter centrifuge is 3600r / min; then use a th...

Embodiment 2

[0047] A kind of extraction method of small molecule pig liver peptide:

[0048] Step 1. Select 10Kg of fresh pork liver, crush it, add 55L of purified water and stir evenly, heat up to 56°C, add 100g of lipase and 120g of neutral protease, and stir at a speed of 68r / min for 30 minutes to obtain the first enzymolysis liquid;

[0049] Step 2: Add 40g of flavored protease, 180g of chicken intestinal digestive tract protease and 1.76g of enzyme activator to the first enzymolysis solution, stir evenly, cool down to 18°C, stir the enzymolysis at a speed of 68r / min, and extract repeatedly 3 times, enzymatic hydrolysis for 1 hour each time, filtering, and combining the extracts from each time to obtain the second enzymatic hydrolysis solution;

[0050] Step 3: Warm up the enzymolysis solution to 92°C, and inactivate the enzyme for 12 minutes; after inactivating the enzyme, use a decanter centrifuge to filter and remove the enzymolysis residue, and the speed of the decanter centrifug...

Embodiment 3

[0057] A kind of extraction method of small molecule chicken liver peptide:

[0058] Step 1. Select 100Kg of fresh chicken liver, crush it, add 600L of purified water and stir evenly, raise the temperature to 58°C, add 1.2Kg of lipase and 1.5Kg of neutral protease, and stir at a speed of 68r / min for 35min to obtain the first enzymatic solution;

[0059] Step 2: Add 500g flavored protease, 2Kg chicken intestinal digestive tract protease and 25g enzyme activator to the first enzymolysis solution, stir evenly, cool down to 20°C, stir enzymolysis at a speed of 68r / min, and repeatedly extract 3 times, each enzymatic hydrolysis for 1.5h, filtered, and each extract was combined to obtain the second enzymatic hydrolysis solution;

[0060] Step 3: Warm up the enzymolysis solution to 95°C, and inactivate the enzyme for 10 minutes; after inactivating the enzyme, use a decanter centrifuge to filter and remove the enzymolysis residue, the speed of the decanter centrifuge is 3600r / min; the...

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PUM

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Abstract

The invention provides an extraction method of small molecule liver peptide. The extraction method comprises following steps: step one, animal liver is mashed, purified water is added, a mixed liquidis stirred uniformly and heated to 54-58 DEG C, lipase and neutral protease are added, stirring enzymolysis is performed for 25-35 min, and a first enzymatic hydrolysate is obtained; step two, flavourzyme, chicken intestinal digestive tract protease and an enzyme activator are added to the first enzymatic hydrolysate and stirred uniformly, a mixture is cooled to 15-20 DEG C and subjected to enzymolysis extraction 2-3 times, enzymolysis time is 1-1.5 h each time, all extracting solutions are combined, and a second enzymatic hydrolysate is obtained; step three, the second enzymatic hydrolysate is heated and subjected to enzyme inactivation, a product is separated, purified, concentrated and lyophilized, and lyophilized small molecule liver peptide powder is obtained. The prepared liver peptide product is high in purity and can be widely applied to the fields of food, healthcare products, drugs and the like, and content of peptide with molecular weight being 180-500 Dalton can reach 99% or higher.

Description

technical field [0001] The invention relates to the technical field of separation of biological peptides, in particular to a method for extracting small molecule liver peptides. Background technique [0002] The liver is an organ in the animal body that mainly has metabolic functions, and plays the roles of deoxidation, storage of glycogen, and synthesis of secreted proteins in the body. Beef liver, pig liver, chicken liver and other animal livers are rich in nutrients such as protein, sugar, minerals and vitamins. Liver polypeptide can be used as a good pharmaceutical chemical raw material and functional food. According to reports, liver polypeptide has natural biological activity, can improve the body's immunity, and also has functions such as anti-oxidation, promoting blood coagulation and inhibiting tumor growth. [0003] Small molecular peptide is a classification of polypeptides. The molecular weight segment is generally below 1000 Daltons. It is also called small pep...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14C07K1/34
CPCC07K1/145C07K1/34C12P21/06
Inventor 卢光明张晓东杨广杨子丰
Owner 河北肽都生物科技集团有限公司
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