Tulipa gesneriana L chromosome and karyotype studying technology

A technique of chromosomes and tulips, which is applied in the field of plant cytology and cytogenetics research, can solve the problems of karyotype research method steps without relatively specific explanations, and achieve the effect of clear dispersion effect

Inactive Publication Date: 2019-02-26
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Xu Ping (Xu Ping. Study on ploidy and hybridization of tulip varieties [D]. Zhejiang University, 2014) observed chromosome ploidy of some tulip varieties, but did not give a relatively specific explanation for the steps of karyotype research methods

Method used

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  • Tulipa gesneriana L chromosome and karyotype studying technology
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  • Tulipa gesneriana L chromosome and karyotype studying technology

Examples

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Effect test

Embodiment 1

[0020] Example 1 Tulip chromosome karyotype research method optimization

[0021] 1. Select 4 representative tulip varieties 'Golden Review', 'Leader', 'Prisma' and 'Purple Flag', and soak the bulbs of the selected varieties in clean water to obtain stem roots. When the root grows to about 1-3cm, in sunny morning (9:00-11:00) and afternoon (14:00-16:00), take the root tip of tulip bulbs of 4 varieties with equal length as experimental materials Ready to spare.

[0022] 2. Put the freshly removed root tip into 0.07 mmol / L cycloheximide for pretreatment, and then fix it with Carnot's fixative (glacial acetic acid:ethanol=1:3) at room temperature for 24 hours. After being taken out from the fixative, the roots were washed with distilled water. If they could not be pressed immediately, the root tips were placed in 70% ethanol and stored at 4°C.

[0023] 3. When making tablets, take out the stored materials, rinse them with distilled water for 3 times, absorb the water, put them ...

Embodiment 2

[0029] Example 2 Tulip Chromosome Karyotype Research

[0030] Applying the method of the present invention, the root tips of 43 varieties of tulips planted in the Beijing Botanical Garden were observed and studied on the chromosome karyotype, all of which achieved good results.

[0031] 1. Material selection: Select robust and complete bulbs without mechanical damage and disease and plant them in a moist substrate of peat: vermiculite: perlite = 1:1:1, and carry out rooting culture at room temperature. Observe once every other day, and when the root length grows to between 1.5cm-2.5cm, select the root tip with vigorous growth and division as the pretreatment material. Collect materials from 9:00 to 11:00 on sunny days.

[0032] 2. Pretreatment: When collecting materials, cut off the thick root tips with scissors, store them in a 2ml centrifuge tube, mark the variety number, and add an appropriate amount of 0.07mmol / L cycloheximide solution, and put it in an ice box for tempor...

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Abstract

The invention relates to a Tulipa gesneriana L chromosome karyotype studying method, wherein specifically the root tip germinated from Tulipa gesneriana L bulb is used as an experimental material, andchromosome morphology observation and studying is performed through the vigorous division characteristic of the meristematic cells in a root tip meristem region. The invention further discloses a Tulipa gesneriana L chromosome karyotype studying technology, which comprises material selection, pretreatment, fixation, dyeing, slide forming and microscopic examination. According to the present invention, with the method and the technology, the Tulipa gesneriana L chromosome is subjected to karyotype studying and analyzing, wherein the method is simple and reliable, the results are accurate and reproducible, and the operation is easy; and the method and the technology have great significance and provide a certain technical support in the Tulipa gesneriana L chromosome karyotype studying, thegermplasm resource classification and protection, and other related researches.

Description

technical field [0001] The invention relates to a tulip chromosome and karyotype research technology, which belongs to the research fields of plant cytology and cytogenetics. Background technique [0002] Plant chromosomes are aggregates containing important genetic information in plant cells, which are rod-shaped or cylindrical when viewed under a microscope. It is named because it is easily stained by basic dyes during cell division, and is widely used in staining observation research. Chromosomal karyotype analysis is a method of analyzing chromosome information by observing and studying chromosome characteristic information such as chromosome number, morphological characteristics, centromere position, and presence or absence of telomeres. [0003] So far, research on karyotype analysis in garden plants has covered lily (Qu Suping. Karyotype research and analysis of some cultivars of lily [D]. Chinese Academy of Agricultural Sciences, 2014.), orchid (Ao Suyan. Guolan Ph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84G01N1/28G01N1/30
CPCG01N1/2813G01N1/286G01N1/30G01N21/84
Inventor 吕英民高星
Owner BEIJING FORESTRY UNIVERSITY
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