Self-oxygenated liposome, preparation method thereof and application of liposome
A liposome and self-oxygenation technology, which is applied in the direction of liposome delivery, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve oxygen consumption, limit the application of photodynamic therapy, and pertinence Weakness and other problems, to achieve the effect of overcoming the impact, good application prospects, and long-lasting release
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Embodiment 1
[0042] Preparation of liposomes
[0043] Step 1: CaO 2 Preparation of NPs:
[0044] (1) Weigh 3g CaCl 2 In the reaction bottle, add 30mL ultrapure water to dissolve;
[0045] (II) Add 15mL of 1M ammonia solution, and add 94mL of PEG200 solution dropwise at a stirring speed of 500r / min;
[0046] (III) Add 15 mL of 30% H 2 o 2 Solution (one drop every 20s);
[0047] (IV) after the dropwise addition, stirring reaction 1h;
[0048] (V) After the reaction is over, take the suspension and centrifuge, the centrifugation conditions are: rotating speed 10000rpm, time 5min, separate and obtain CaO 2 NP.
[0049] Step 2: Preparation of Azo-BODIPY:
[0050] The synthetic route of Azo-BODIPY is as follows:
[0051]
[0052] The synthesis steps are:
[0053] 1) Weigh 1.10g of 2 into a flask, add 10mL of ethanol and stir to dissolve, then add 1.03g of 1, add 4mL of 10% NaOH aqueous solution dropwise into the flask under rapid stirring conditions, stir and react at 30°C for 9h, af...
Embodiment 2
[0070] TEM images of liposomes
[0071] The prepared liposomes were photographed by a transmission electron microscope, and the specific test steps were as follows: 20 μL of a sample solution with a concentration of 0.2 mg / mL was dropped on a copper grid, and the morphology test was performed after the sample was dried. its image as figure 1 As can be seen from the figure, liposomes have better dispersion in aqueous solution, and their particle size is about 80nm, which is conducive to entering cells and further providing the possibility of oxygen supply for organisms.
Embodiment 3
[0073] Test of the change of dissolved oxygen before and after light
[0074] A liposome dispersion of 80 μg / mL was prepared, and the solvent was ultrapure water. After 20 minutes, on the basis of the original device, use 730nm laser irradiation to test the change of dissolved oxygen in the dispersion. The obtained image of the change of dissolved oxygen before and after illumination is as follows: figure 2 As shown in the figure, it can be seen from the figure that the oxygen content in the solution does not change significantly before irradiation, but after irradiating with a 730nm laser, the oxygen content in the solution increases significantly within a certain period of time. The experimental results show that the synthesized liposome can accelerate the production of oxygen under the condition of light excitation of specific wavelength, so it can be well used in photodynamic therapy.
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