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Preparation method of low-endotoxin collagen

Endotoxins are removed through physical and chemical means, including expansion, planing, hydrolysis and alcohol washing steps, which solves the problem of difficult endotoxin removal in traditional collagen production, achieves efficient production of low endotoxin collagen, and reduces production costs and time.

Active Publication Date: 2019-03-01
GUANGDONG VICTORY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This dialysis procedure is not only very time-consuming, energy-consuming and material-consuming, but also greatly reduces the production capacity of low-endotoxin collagen, thus increasing the production cost multiples

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] refer to figure 1 , which is a flowchart of a method for preparing low-endotoxin collagen according to an embodiment of the present invention. Such as figure 1 As shown, the preparation method of low endotoxin collagen of the present invention comprises the following steps: providing animal skin (step S101); removing residual fat tissue and muscle tissue (step S103); expanding animal skin (step S105); removing part of animal skin (step S107); hydrolysis of animal skin (step S109); salting out (step S111); and alcohol washing (step S113).

[0021] In step S101, the provided animal skin can be obtained from vertebrates whose outer skin system includes skin and skin derivatives, including but not limited to bovine, porcine, equine, Livestock or fish such as sheep, chickens, ducks, turkeys, geese, whales or sharks.

[0022] In an embodiment, the provided animal skin may be the subcutaneous tissue from which the epidermal tissue has been manually or mechanically removed. ...

example 1

[0032] After the 4000~6000g bovine skin that has removed the epidermal tissue removes the residual adipose tissue and muscle tissue on the animal skin, the calcium hydroxide solution with a weight percentage of about 13% (water and calcium hydroxide is 100:14 within the range of about 100:16) to expand to 1.2 times of the original, and then use a 3000mm skin slicer from MOSCONI Company at room temperature to shave a thickness of 0.5mm from the upper and lower surfaces of the skin.

[0033]Put the skin obtained from the above steps in 1000 to 3000 ml of 10% to 20% isopropanol solution, and grind it into small pieces with a meat grinder. Add 12-20 g of pepsin, then add 480-720 ml of glacial acetic acid to adjust the pH value of the solution to 1.5-3.5, then stir at 1°C to 12°C for 24-48 hours to obtain a mixture.

[0034] Add 3600-5700ml of sodium chloride solution to the above mixture for salting out, collect the precipitate after standing for 12-72 hours, and wash the obtained...

example 2

[0036] 800-1600 g of collagen 2 was obtained by the same preparation method as in Example 1, except that the skin was swollen to 2.1 times the original size and a thickness of 0.2 mm was removed from the upper surface of the skin.

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Abstract

The invention discloses a preparation method of low-endotoxin collagen. The preparation method comprises the following steps: providing animal skin; removing residual adipose tissues and muscular tissues of the animal skin; swelling the animal skin by using an alkali solution; excluding the upper and lower surfaces of the animal skin; hydrolyzing the animal skin by using a solution containing hydrolase; separating out the low-endotoxin collagen by using a salt solution; and washing the low-endotoxin collagen by using an alcohol solution.

Description

technical field [0001] The present invention relates to a preparation method of collagen, in particular to a preparation method of low endotoxin collagen. Background technique [0002] Endotoxin is a component of the cell wall surface of Grainella bacteria, which is called lipopolysaccharide (lipopolysaccharide), which is released only when the bacteria die and dissolve or the bacterial cells are artificially destroyed. The lipopolysaccharide molecule is composed of three parts: specific polysaccharide, core polysaccharide and lipid A. Lipid A is the major toxic component of endotoxin. Lipid A structures of different Gram-negative bacteria are basically similar. Therefore, for all infections caused by Gram-negative bacteria, although the strains are different, the endotoxin effect of each bacteria is roughly the same. It mainly causes clinical symptoms such as fever, leukocytosis, microcirculation disturbance, and shock in the human body. [0003] In the medical-grade co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/30C07K1/14C12P21/06
CPCC07K14/78C12P21/06
Owner GUANGDONG VICTORY BIOTECH CO LTD