A preparation method and application of magnetic nanoparticles for capturing exosomes in blood

Abstract

The invention discloses a preparation method and application of magnetic nanoparticles for capturing exosomes in blood, which is characterized by the following steps: (1) mixing ferrous chloride hexahydrate and ferrous chloride tetrahydrate In distilled water, stir and mix well and heat to 70°C, then slowly add ammonia solution dropwise, after the hydrolysis is basically completed, slowly add cetyltrimethylamine bromide to the solution, stir and centrifuge to collect the product for washing, take the lower precipitate and dry it in vacuum to obtain Nano Fe 3 o 4 Particles; (2) take nano Fe 3 o 4 After the particles were dispersed in an aqueous ethanol solution and ultrasonically treated, after adding tetraethyl orthosilicate solution, they were gradually added to an aqueous ammonia water bath to react for 5 h, and the product was collected by centrifugation, washed and dried to obtain SiO coated particles. 2 In addition, irregular magnetic micro-nano particles with a pore size of 40-200 nm are distributed on the surface, which has the advantages of binding exosomes without damage to the greatest extent, and the sample volume used is small, the processing time is short, and the operation is simple.

Description

technical field

[0001] The invention belongs to the field of tumor detection, and in particular relates to a preparation method and application of magnetic nanoparticles for capturing exosomes in blood. Background technique

[0002] In recent years, due to changes in the environment and lifestyle, the incidence of malignant tumors (also known as cancer) in my country has increased significantly. At the same time, the number of deaths from malignant tumors in my country is also increasing, which is largely due to the fact that most patients have reached the middle and late stages of cancer when they are clearly diagnosed, and have lost the best time for treatment. The survival rate of advanced cancer patients after treatment is extremely low, and the survival time is short, while the cure rate of early cancer patients is high, about 80%-90%. Therefore, early diagnosis is particularly important for cancer diagnosis and treatment. Currently, the diagnostic methods for maligna...

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