Centrifugal ultrafiltration sample handling device and use method thereof

A sample processing and centrifuge technology, which is applied in the direction of measuring devices, test sample preparation, sampling, etc., can solve the problems of low efficiency and slow dialysis speed, and achieve the effects of avoiding sample contamination, improving purity, and simple facilities

Active Publication Date: 2019-03-01
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a centrifugal ultrafiltration sample processing device and its use method to solve the problems of slow dialysis speed and low efficiency of existing dialysis devices

Method used

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  • Centrifugal ultrafiltration sample handling device and use method thereof
  • Centrifugal ultrafiltration sample handling device and use method thereof
  • Centrifugal ultrafiltration sample handling device and use method thereof

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Embodiment 1

[0046] Such as Figure 4 As shown, the ultrafiltration rotor cup 4 of the centrifugal ultrafiltration sample processing device in the present embodiment is parabolic, with an inner diameter of 5 cm and a height of 15 cm; the ultrafiltration rotor cup 4 has a three-layer structure, such as figure 2 As shown, the inner clamping layer 15 is a mixed cellulose ester membrane with a pore diameter of 0.22 microns, the dialysis membrane layer 17 is a cellulose membrane with a molecular weight cut-off of 500 Da, and the outer clamping layer 16 is made of polytetrafluoroethylene with a pore diameter of 2 microns; One pump system 1 is A pump 13, B pump 14 binary constant flow pump, the maximum flow rate is 10ml / min; the centrifuge 7 can rotate forward or reverse, the maximum speed is 3000r / min; the solvent cup 8 is a round table type, the inner diameter of the upper bottom is 10cm, the inner diameter of the lower bottom is 18cm, and the height is 18cm. The round table type solvent cup 8...

Embodiment 2

[0056] Such as Figure 5 As shown, the ultrafiltration rotor 4 of the centrifugal ultrafiltration sample processing device in this embodiment is cylindrical, with an inner diameter of 55 cm and a height of 35 cm; the walls of the ultrafiltration rotor 4 are composed of 4 layers, and the inner holding layer 15 is an aperture. 10 micron polypropylene material, the dialysis membrane layer 17 is a polyethersulfone membrane with a molecular weight cut-off of 1kDa, the outer clamping layer 16 is made of polypropylene with a pore diameter of 2 microns, and the outer clamping layer 16 is made of polypropylene with a pore diameter of 1 mm. Both the first pump system 1 and the C pump 11 are constant flow pumps. The maximum rotational speed of the centrifuge 7 is 3000r / min; the circular truncated shell can reduce the splashing and splashing of the solvent during the centrifugation process. The device of the invention can retain substances with a molecular weight greater than 1kDa, and c...

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Abstract

The invention provides a centrifugal ultrafiltration sample handling device and a use method thereof. The centrifugal ultrafiltration sample handling device includes a pump system for outputting and recovering materials, a rotating cup of a multilayer film structure, a liquid level detector, and a centrifuge system supplying rotary power to the ultrafiltration rotating cup, wherein a centrifuge isconnected with the ultrafiltration rotating cup through a connecting part of a rotating shaft. According to the centrifugal ultrafiltration sample handling device and the use method thereof, a feeding pump is used for continuously adding samples, macromolecules in the samples are trapped in the rotating cup in the centrifugal process, small molecule compounds and salts in the samples are rapidlythrown out of the ultrafiltration rotating cup, and the samples can be cleaned by adding a solvent to the feeding pump; and compared with conventional dialysis and ultrafiltration, the centrifugal ultrafiltration sample handling device has the advantages of simple configuration, convenient operation, large sample handling capacity, high concentration multiple, complete dialysis and the like, and the dialysis efficiency is greatly improved through a high-speed centrifugal mode, so that the time and labor costs are greatly saved.

Description

technical field [0001] The invention belongs to the technical field of biomedical purification devices, and in particular relates to a centrifugal ultrafiltration sample processing device and a use method thereof. Background technique [0002] At present, in the field of biomedicine, the purification process of biological samples such as proteins and polysaccharides often requires steps such as desalting, depigmentation, impurity protein removal, sample concentration, and molecular interception to refine samples. Dialysis membrane filtration is currently the most widely used and most convenient. The lowest cost means of implementation. Usually, the sample to be purified is placed in a dialysis membrane bag, and the two ends are clamped with sealing clips to keep the sample in the dialysis membrane bag in a closed state, and then the entire dialysis membrane bag is placed in a container filled with solvent, and the container is equipped with The magnetic stirring bar acceler...

Claims

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Application Information

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IPC IPC(8): G01N1/40
CPCG01N1/4005G01N2001/4016
Inventor 李笃信易琳孙瑞琪陆菲菲张真庆
Owner SUZHOU UNIV
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