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Method for measuring terpene lactone content in ginkgo biloba extract

A technology of terpene lactone and extract, which is applied in the field of determination of terpene lactone content in Ginkgo biloba extract, can solve the problems of poor detector stability, high price, large amount of reference substances, etc., to reduce result errors, The effects of stable properties and reduced detection costs

Inactive Publication Date: 2019-03-01
JIANGSU HUIKANG BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since ELSD and RID detectors have high requirements for detection conditions, under general laboratory conditions, the precision of the detection results (peak area or result content) is difficult to meet the requirements of RSD≤2%, and the stability of the detector is poor
And the use of reference substances is relatively large, the price is expensive, and the detection cost is relatively high

Method used

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  • Method for measuring terpene lactone content in ginkgo biloba extract
  • Method for measuring terpene lactone content in ginkgo biloba extract
  • Method for measuring terpene lactone content in ginkgo biloba extract

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Experimental program
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Effect test

Embodiment 1

[0020] 1 Instruments and reagents

[0021] 1.1 Instrument

[0022] Electronic analytical balance (Mettler XS105), Agilent 1260 liquid chromatograph, Alltech ELSD6000 evaporative light scattering detector, chromatographic column: Agilent C18-EPS 5μm 4.6mm×250mm.

[0023] 1.2 Reagents

[0024] Methanol (analytically pure), tetrahydrofuran (chromatographically pure), n-propanol (chromatographically pure), twice distilled water, unless otherwise specified, all reagents used in this experiment are analytically pure.

[0025] 2 methods

[0026] 2.1 Preparation of reference solution

[0027] Take appropriate amounts of bilobalide reference substance, ginkgolide A reference substance, ginkgolide B reference substance and ginkgolide C reference substance respectively, weigh them accurately, and add methanol to make 4mg, 1mg, 3mg, 2mg per 1ml. The mixed solution is used as the 1# reference substance solution. Precisely pipette 5ml of the 1# solution and add 5ml methanol and mix it u...

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Abstract

The invention discloses a method for measuring terpene lactone content in ginkgo biloba extract, and belongs to the field of chemical detection, and aims to provide an HPLC-ELSD internal standard detection method with good reproducibility and less interference factor for convenient and quick measurement of the terpene lactone content in ginkgo biloba, which uses imidacloprid as an internal standard substance, and adopts the HPLC-ELSD method to measure each constituent in the terpene lactone and calculate the content of the constituents. The method for measuring terpene lactone content in ginkgo biloba extract can replace the method of detecting the terpene lactone content in ginkgo leaves or in ginkgo biloba extract in the pharmacopoeia.

Description

technical field [0001] The invention relates to a method for measuring the content of terpene lactones in ginkgo biloba extract, which belongs to the field of chemical detection. Background technique [0002] Ginkgo biloba extract (GBE) is a light yellow to brown powdery substance extracted and purified from the dried leaves of Ginkgo biloba, a plant of the genus Ginkgo biloba. It mainly contains total flavonol glycosides and terpene lactones. Ginkgo biloba extract has the functions of dilating coronary blood vessels and cerebral blood vessels, increasing coronary flow and cerebral blood flow, and improving heart and brain function. It can improve the symptoms and memory function caused by cerebral ischemia. It has the effect of relieving bronchial smooth muscle spasm, which may be related to the antagonism of bilobalide to platelet activating factor. This product is suitable for the treatment of coronary heart disease, angina pectoris, cerebral vasospasm, cerebral insuff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/045
Inventor 崔升官陈金平
Owner JIANGSU HUIKANG BIOLOGICAL