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Aflatoxin B1 control compound biological agent and application thereof

A technology of aflatoxin and biological preparations, applied in the field of feed, can solve problems affecting animal safety and palatability, poor practicality of physical detoxification, and inability to meet production needs, so as to enhance the body's immunity, reduce use, and maintain intestinal tract Healthy effect

Active Publication Date: 2019-03-05
北京科为博生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the practicability of physical detoxification is poor, and chemical detoxification has problems affecting animal safety and palatability, which cannot meet production needs

Method used

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  • Aflatoxin B1 control compound biological agent and application thereof
  • Aflatoxin B1 control compound biological agent and application thereof
  • Aflatoxin B1 control compound biological agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Screening and identification of antagonistic strains of Aspergillus flavus and bacterial strains degrading aflatoxin B1

[0034] 1 Material method

[0035] 1.1 Test material

[0036] 1.1.1 Reagents

[0037] Coumarin, Aflatoxin B 1 , Aflatoxin B 1 Detection kit.

[0038] 1.1.2 Medium

[0039] (1) Potato dextrose agar medium

[0040] Potato dextrose agar medium 38g, distilled water (ddH 2 O) 1000mL, sterilize at 121°C for 30min.

[0041] (2) Potato Dextrose Broth Liquid Medium

[0042] Potato dextrose broth medium 35g, distilled water (ddH 2 O) 1000mL, autoclave at 121°C for 30min.

[0043] (3) Liquid activated medium

[0044] Peptone 10g, yeast powder 5g, sodium chloride 10g, distilled water (ddH 2 O) 1000mL, autoclave at 121°C for 30min.

[0045] (4) Primary screening culture medium

[0046] Potassium dihydrogen phosphate 0.25g, magnesium sulfate heptahydrate 0.25g, potassium nitrate 0.50g, ammonium sulfate 0.50g, calcium chloride 0.005g, ferric ch...

Embodiment 2

[0078] Embodiment 2 controls aflatoxin B 1 Preparation of complex biologics

[0079] 1. the production method of bacillus subtilis or bacillus licheniformis comprises the steps:

[0080] (1) Inoculate Bacillus subtilis or Bacillus licheniformis on the slant medium, and culture at 30-40° C. for 12-24 hours.

[0081] (2) Inoculate the Bacillus subtilis or Bacillus licheniformis colony cultivated on the slant in the seed medium, the liquid filling volume is 50-150mL / 500mL, and cultivate 12- 24 hours.

[0082] (3) Inoculate the seed solution of Bacillus subtilis or Bacillus licheniformis in the seed tank with a weight ratio of 0.1-0.5%, and the filling volume is 50-150L / 500L, at 30-40°C and 120-180 rpm Incubate for 12-24 hours.

[0083] (4) Inoculate the seed tank culture solution in the fermenter with a weight ratio of 0.1-0.5%, the tank pressure is 0.01-0.05MPa, the stirring speed is 120-180 rpm, and the ventilation ratio is 0.5-1:0.1-0.5( volume ratio), the fermentation ti...

Embodiment 3

[0094] Embodiment 3 Aflatoxin B 1 detox effect

[0095] Adding different detoxification products to moldy feed to compare aflatoxin B 1 As for the detoxification effect, it can be seen from Table 2 that the degradation rate of aflatoxin B1 by the composite biological preparation is significantly higher than that of the yeast cell wall.

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Abstract

The invention discloses an aflatoxin B1 control compound biological agent and application thereof. The aflatoxin B1 control compound biological agent is formed by compounding bacillus subtilis, bacillus licheniformis, enterococcus faecium and yeast cell wall, wherein the bacillus subtilis, the bacillus licheniformis and the enterococcus faecium can effectively inhibit growth of mould in feeds. Theaflatoxin B1 control compound biological agent effectively combines two feed-contained aflatoxin B1 detoxification methods, namely, biodegradation and bio-absorption; the contained bacillus subtiliscan effectively degrade aflatoxin B1, and the contained yeast cell wall can effectively absorb aflatoxin B1. Besides, the aflatoxin B1 control compound biological agent can not only achieve mould preventing and cleaning functions, but also achieve microbial healthcare effects.

Description

technical field [0001] The invention relates to the technical field of feed, in particular, the invention relates to a method for controlling aflatoxin B 1 The preparation and application method of the composite biological preparation. Background technique [0002] Aflatoxins are the most toxic type of mycotoxins, widely present in grain crops such as moldy peanuts and corn, as well as meat and milk foods. [0003] Aflatoxins are mainly a large class of structurally similar secondary metabolites produced by pathogenic fungi such as Aspergillus flavus and Aspergillus parasiticus. There are 18 kinds of aflatoxins and their derivatives that have been discovered so far, including aflatoxin B 1 , B 2 , G 1 , G 2 , ML, M 2 and B 2a , G 2a ,P 1 , Q 1 , R 0 etc., except aflatoxin B 1 , B 2 and G 1 , G 2 Except for natural production, the rest are their derivatives. Aflatoxin B 1 It is the most toxic. It has severe acute toxicity and obvious chronic toxicity to anima...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12R1/10C12R1/125C12R1/46
CPCA23L5/20C12N1/16C12N1/20
Inventor 李富伟吴培均韩明渠杨忠广
Owner 北京科为博生物科技有限公司
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