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A kind of probiotic feed with high viable bacteria count prepared by fermenting liquor distiller's grains with Lactobacillus plantarum and its method

A technology with a high number of viable bacteria and probiotics, applied in animal feed, animal feed, applications, etc., can solve the problems of high water content of fresh distiller's grains, conversion of distiller's grains resources, pollution of the environment, etc., to prevent intestinal infection of animals and improve immunity System, the effect of promoting intestinal development

Active Publication Date: 2022-03-04
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fresh distiller's grains have high moisture content and high acidity. If they are allowed to discharge, they will deteriorate if they are not treated in time, which not only seriously pollutes the environment, but also is a great waste of resources.
At present, distiller's grains are mostly used to develop animal feed, produce vinegar, cultivate edible fungi, etc., but still cannot fully convert the huge distiller's grain resources

Method used

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  • A kind of probiotic feed with high viable bacteria count prepared by fermenting liquor distiller's grains with Lactobacillus plantarum and its method
  • A kind of probiotic feed with high viable bacteria count prepared by fermenting liquor distiller's grains with Lactobacillus plantarum and its method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] In this embodiment, the method for preparing probiotic feed with a high viable count by using Lactobacillus plantarum to ferment liquor distiller's grains is as follows:

[0020] Mix 74% dried distiller's grains, 20% eucommia leaf powder, 2% fructooligosaccharides, 2% inulin and 2.0% ammonium sulfate, then moisten and steam the clinker, cool the clinker to 36°C and knead it quickly, Stir evenly, inoculate Lactobacillus plantarum suspension (8%, m / v) and anaerobically ferment at 36°C for 4 days, and vacuum dry at 45°C after fermentation to obtain fermented probiotic feed.

[0021] After testing, the lactic acid content in the finished feed can reach 5.11%, and the Lactobacillus plantarum can reach 1×10 10 CFU / g feed, the content of chlorogenic acid can reach 0.65%. The content of lactic acid and the number of viable bacteria were investigated and measured at room temperature (25°C). The results are shown in Table 1.

[0022] Table 1 Changes in lactic acid content, viab...

Embodiment 2

[0026] The high viable count probiotic feed prepared by using Lactobacillus plantarum to ferment liquor distiller's grains in this embodiment comprises the following raw materials in parts by weight: dried distiller's grains 70, Eucommia leaf powder 15, ammonium sulfate 0.5, fructooligosaccharides 0.5 and Inulin 0.5.

[0027] The preparation method of the described high viable count probiotic feed, the steps are as follows:

[0028] (1) Dry the wet distiller's grains obtained after liquor brewing to obtain dry distiller's grains with a moisture content of less than 10%, add pulverized Eucommia leaf powder, ammonium sulfate, fructooligosaccharides and inulin to the dry distiller's grains, and mix them evenly in proportion Carry out moistening and steaming, the amount of water added when moistening and steaming is 30% of the weight of the material, the steaming temperature is 100°C, and the steaming time is 25min;

[0029] (2) Cool to 30°C after steaming, inoculate Lactobacillu...

Embodiment 3

[0032] The high viable count probiotic feed prepared by using Lactobacillus plantarum to ferment liquor distiller's grains in this embodiment comprises the following raw materials in parts by weight: dried distiller's grains 85, Eucommia leaf powder 30, ammonium sulfate 3.0, fructooligosaccharides 2 and inulin2.

[0033] The preparation method of the described high viable count probiotic feed, the steps are as follows:

[0034] (1) Dry the wet distiller's grains obtained after liquor brewing to obtain dry distiller's grains with a moisture content of less than 10%, add pulverized Eucommia leaf powder, ammonium sulfate, fructooligosaccharides and inulin to the dry distiller's grains, and mix them evenly in proportion Carry out moistening and steaming, the amount of water added when moistening and steaming is 30% of the weight of the material, the steaming temperature is 100°C, and the steaming time is 25min;

[0035] (2) Cool to 38°C after steaming, inoculate Lactobacillus pla...

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Abstract

The invention discloses a probiotic feed with high viable bacteria count and a method prepared by fermenting liquor distiller's grains with Lactobacillus plantarum. The probiotic feed with high viable count is made of the following raw materials in parts by weight: dried distiller's grains 70-85 , Eucommia leaf powder 15-30, ammonium sulfate 0-3.0, fructo-oligosaccharide 0.5-2 and inulin 0.5-2. The preparation method is as follows: add Eucommia ulmoides leaf powder, ammonium sulfate, fructooligosaccharide and inulin to the dried distiller's grains, mix evenly, and steam the material with moist water; after steaming, inoculate Lactobacillus plantarum suspension for anaerobic solid-state fermentation to obtain High viable count probiotic feed. The invention utilizes the strong ability of Lactobacillus plantarum to decompose sugars, degrades the macromolecular sugars in distiller's grains and Eucommia leaves, and increases the content of crude protein, small peptides, oligosaccharides and monosaccharides in feed; and after Eucommia leaves are fermented, It also increases the content of chlorogenic acid in the feed, while adding prebiotics also increases the proliferation of Lactobacillus plantarum in the intestinal tract of animals.

Description

technical field [0001] The invention belongs to the field of deep processing of agricultural and forestry by-products, and belongs to a probiotic feed which can improve feed palatability and intestinal microecological balance by using distiller's grains and eucommia leaves as raw materials and fermenting with plantaractobacillus. Background technique [0002] The abuse of antibiotics in the aquaculture industry is increasingly rejected by the market, and the scientific development of new and safe probiotic feeds is an urgent task for people's awareness of food safety, environmental protection and feed safety. Probiotic fermented feed is a mixture containing feed raw materials, bacteria and their metabolites obtained after inoculating probiotic strains on feed materials and fermenting them. The feed fermented by probiotics can not only change the composition of the feed, but also improve the intestinal micro-ecological balance of animals and enhance their immunity. However, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12A23K10/38A23K10/30A23K20/163A23L3/3463
CPCA23K10/12A23K10/30A23K10/38A23K20/163A23L3/3463Y02P60/87
Inventor 张丽华安明哲王小媛李珍珠刘梦培胡晓龙纵伟
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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