Fat storage liquid and preparation method thereof

A technology for preserving fluid and fat, which is applied in the field of preserving fluid for preserving fat samples, can solve the problems of affected stem cell acquisition rate and activity, short preservation time of fat samples, etc., and achieves the effects of simple preparation, low cost, and improved efficiency.

Inactive Publication Date: 2019-03-22
QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problems of short storage time of fat samples and the influence of stem cell acquisition rate and activity in the prior art, and provide a fat storage solution, which is simple in proportion, low in cost and easy to use

Method used

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  • Fat storage liquid and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A fat preservation solution provided by the invention, each liter of fat preservation solution is composed of the following components by weight: high-sugar DMEM dry powder medium 13.4g, sodium bicarbonate 2.438g, insulin 10mg, penicillin 200mg, streptomycin 200mg, amphoteric Vitamin C 5mg, aspirin 400mg, vitamin C 700mg, resveratrol 12mg, icariin 10mg, hydrocortisone 40ug, VEGF 20μg, IGF-I 10μg, EPO 10μg, and the balance was water for injection.

[0018] The preparation method of this fat preservation solution is as follows:

[0019] (1) Pour 13.4 g of high-sugar DMEM dry powder medium (brand: GIBCO, product number 12100-046) into a container, wash off the residual medium in the bag with a small amount of water for injection, and put it into the container. Add water for injection to 950ml, stir gently to dissolve;

[0020] (2) Add 2.438 g sodium bicarbonate;

[0021] (3) Gently stir to dissolve, add water for injection to 1L;

[0022] (4) adjust the pH value to neut...

Embodiment 2

[0026] A kind of fat preservation solution provided by the invention, every liter of fat preservation solution is composed of the following components by weight: high sugar DMEM dry powder culture medium 13.4g, sodium bicarbonate 2.438g, insulin 2mg, penicillin 100mg, streptomycin 100mg, amphoteric Vitamin C 2.5mg, Aspirin 200mg, Vitamin C 300mg, Resveratrol 7mg, Icariin 2mg, Hydrocortisone 20ug, VEGF 10μg, IGF-I 5μg, EPO 5μg, and the balance was water for injection.

[0027] The preparation method of this fat preservation solution is as follows:

[0028] (1) Pour 13.4g of high-sugar DMEM dry powder medium into a container, wash off the residual medium in the bag with a small amount of water for injection, and put it into the container. Add water for injection to 950ml, stir gently to dissolve;

[0029] (2) Add 2.438 g sodium bicarbonate;

[0030] (3) Gently stir to dissolve, add water for injection to 1L;

[0031] (4) adjust the pH value to neutrality with 1mol / L sodium hy...

Embodiment 3

[0035] A kind of fat preservation solution provided by the invention, every liter of fat preservation solution is composed of the following components by weight: high-sugar DMEM dry powder culture medium 13.4g, sodium bicarbonate 2.438g, insulin 5mg, penicillin 150mg, streptomycin 150mg, amphoteric Vitamin C 4mg, aspirin 300mg, vitamin C 400mg, resveratrol 8mg, icariin 3mg, hydrocortisone 30ug, VEGF 15μg, IGF-I 6μg, EPO 6μg, and the balance was water for injection.

[0036] The preparation method of this fat preservation solution is as follows:

[0037] (1) Pour 13.4 g of high-sugar DMEM dry powder medium into a container, wash off the residual medium in the bag with a small amount of water for injection, and put it into the container. Add water for injection to 950ml, stir gently to dissolve;

[0038] (2) Add 2.438 g sodium bicarbonate;

[0039] (3) Gently stir to dissolve, add water for injection to 1L;

[0040] (4) adjust the pH value to neutrality with 1mol / L sodium hyd...

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Abstract

The invention discloses fat storage liquid, which is used for storing a fat tissue sample and is prepared from the following ingredients of high-sugar DMEM dry powder culture media, sodium bicarbonate, insulin, penicillin, streptomycin, fungizone, aspirin, vitamin C, resveratrol, icariin, hydrocortisone, VEGF, IGF-I and EPO. The fat storage liquid can be easily prepared; the cost is low; the use is convenient; the activity of stem cells in the fat tissues can be maintained for a long time; the time limitation of the tissue sample from collection to preparation is greatly reduced; the pollutionprobability can be effectively reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preservation solution for preserving fat samples. Background technique [0002] Mesenchymal stem cells (MSCs) are a kind of stem cells with multilineage differentiation potential, which have the potential to differentiate into a variety of mesoderm cell lineages, including osteoblasts, adipocytes, chondrocytes, etc. In the normal injury repair process of the body, MSCs can be recruited to the injury site under the induction of chemokines, proliferate and differentiate locally, and participate in injury repair and tissue regeneration through paracrine effects. Adipose tissue-derived MSCs are easy to isolate and expand in vitro, and maintain multi-directional differentiation potential after multiple divisions and passages. They are ideal seed cells for cell therapy and regenerative medicine. Adipose tissue is widely distributed, easy to collect, has no ethical issues, basically pain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 张炳强孙亚如王福斌王二朴李翠翠李乐兴陈梦梦
Owner QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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