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A temperature-responsive rabies vaccine delivery system

A technology of rabies vaccine and delivery system, applied in the intersection of biological preparations and biomedicine, can solve the problems of complex manufacturing process of microsphere adjuvant, poor immune effect of adjuvant, long residence time of gel adjuvant, etc.

Active Publication Date: 2022-07-26
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, vaccine adjuvant research is also one of the hotspots, including traditional aluminum adjuvants, new microsphere adsorption adjuvants, gel adjuvants such as chitosan, etc. All kinds of adjuvants have their own characteristics and shortcomings: The effect is not good, the manufacturing process of microsphere adjuvant is complicated, and the gel adjuvant has the characteristics of long residence time and controllable release
[0004] The immunization method of clinical application of rabies vaccine is immunization injection on the 0th, 3rd, 7th, 14th, and 28th day, which has the disadvantages of many injections and low immune effect. With the progress of the people-oriented social concept, how to reduce the pain of patients and improve Compliance is one of the directions of medical progress

Method used

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  • A temperature-responsive rabies vaccine delivery system
  • A temperature-responsive rabies vaccine delivery system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1. Preparation of rabies vaccine delivery system

[0039] 1. Preparation of temperature-sensitive polymer material solution

[0040] At room temperature, PLGA-PEG-PLGA (purchased from Jinan Daigang Bioengineering Co., Ltd., customized products have no product number, wherein the molecular weight of PLGA is about 2000, the molecular weight of PEG is about 1500; phase transition occurs at 30 °C) by mass volume fraction 20% (g:ml) ) is dissolved in physiological saline (physiological saline is a sodium chloride aqueous solution with a concentration of 0.9%) to obtain a PLGA-PEG-PLGA hydrosol (the mass volume ratio of PLGA-PEG-PLGA in the hydrosol is 20%; the meaning of the mass volume fraction 20% Dissolve 20g of solute in an appropriate amount of solvent and dilute to 100ml).

[0041] The specific dissolution method is as follows: PLGA-PEG-PLGA is added with normal saline at room temperature, fully swollen at 4°C for 24h, and then dissolved alternately for 72h...

Embodiment 2

[0049] Example 2. Evaluation of immune effect of rabies vaccine delivery system

[0050] Subcutaneous administration of BALB / C mice was used to evaluate the immune effect of the temperature-sensitive delivery system for rabies vaccine prepared in Example 1, and the mice were grouped and administered according to Table 1.

[0051] Table 1 Animal grouping and dosage table

[0052]

[0053] The dose of rabies vaccine solution group and high-dose group was 10IU / kg, and the low-dose group was 1IU / kg. The immunization procedure of rabies vaccine solution group was the same as that used in clinical practice. The multiple times of immunization in the delivery system group were as follows: 0 and 14 days for 2 times of immunization, and 0, 14, and 28 days for 3 times of immunization.

[0054] In the above, the rabies vaccine thermosensitive delivery system is prepared according to the following method without manganese:

[0055] After dissolving the rabies vaccine antigen with physio...

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Abstract

The invention discloses a temperature-responsive rabies vaccine delivery system. The invention provides a temperature-sensitive delivery system for rabies vaccine, comprising the following solutes: PLGA-PEG-PLGA, manganese ion and rabies vaccine antigen; the ratio of PLGA-PEG-PLGA, manganese ion and rabies vaccine antigen is 50- 200mg: 20‑100µg: 0.3‑5IU. In the delivery system prepared by the invention, the amount of antibody produced after the second injection can reach the effect after the fourth injection of the commercially available rabies vaccine; when the antigen contained in the delivery system is reduced to one tenth of the dose of the commercially available rabies vaccine, the injection is performed , producing antibody titers comparable to those of commercially available vaccine products. The rabies vaccine delivery system of the present invention can reduce the number of immunizations and maintain the original immune response intensity on the basis that the single dose of the same commercially available rabies vaccine remains unchanged.

Description

technical field [0001] The invention belongs to the cross field of biological preparations and biomedicine, and particularly relates to a temperature-responsive rabies vaccine delivery system. Background technique [0002] Rabies is a fatal disease caused by rabies virus infection, and the mortality rate after the onset is almost 100%. So far, there is still no effective treatment method in the world. Therefore, anyone who is bitten by a rabid dog or an animal that is not sure whether it is poisonous needs to be injected with rabies vaccine immediately. Most of the rabies vaccine is used for post-exposure immunization, so the faster the immune response is generated in the human body, the higher the antibody titer, and the better the preventive effect. [0003] At present, vaccine adjuvant research is also one of the hot spots, including traditional aluminum adjuvant, new microsphere adsorption adjuvant, gel adjuvant such as chitosan, etc. Each type of adjuvant has its own c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/205A61K47/34A61K47/02A61P31/14A61K39/39
CPCA61K39/12A61K39/39A61K47/02A61K47/34A61P31/14A61K2039/55505C12N2760/20134
Inventor 徐东刚付文亮景浩然邢微微
Owner ACADEMY OF MILITARY MEDICAL SCI