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Method for detecting content of bisphenol A by electrochemical luminescent adaptor sensor

A detection method and aptamer technology, applied in the direction of material electrochemical variables, biochemical equipment and methods, measuring devices, etc., to achieve the effect of enhancing the detection signal

Active Publication Date: 2019-03-26
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In earlier research, we developed a signal-off ECL sensor based on the recognition of BPA and aptamers for the sensitive detection of BPA [14] , however, the signal-off model may get false positive results [15] , so it is necessary to find a way to solve this problem

Method used

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  • Method for detecting content of bisphenol A by electrochemical luminescent adaptor sensor
  • Method for detecting content of bisphenol A by electrochemical luminescent adaptor sensor
  • Method for detecting content of bisphenol A by electrochemical luminescent adaptor sensor

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Experimental program
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Embodiment

[0045] an experimental part

[0046] 1 reagent

[0047] Tris(1,10-phenanthroline) dichloride ruthenium(II) hydrate (Ru(phen) 3 2+ ), chloroauric acid tetrahydrate (HAuCl 4 4H 2 O) and K 3 Fe(CN) 6 BPA, bisphenol S (BPS), bisphenol F (BPF), progesterone (PRG) and hydroquinone (HQ) were purchased from Sigma-Aldrich (St.Louis, MO , USA); dimethylsulfoxide (DMSO), tripropylamine (TPA), tris(2-carboxyethyl)phosphine (TCEP) and 6-mercaptohexanol (MCH) were purchased from Aladdin (Shanghai, China).

[0048] The oligonucleotides in this study were synthesized by Shanghai Sangong (Shanghai, China), and the sequence is as follows: (5'-3')

[0049] Capture DNA (CDNA): SH-TTT TTT TTT TCGTGATGCGCTGGGCCATACGCGGAACGCTAT

[0050] BPA aptamer: CCG GTG GGT GGT CAG GTG GGA TAG CGT TCC GCG TAT GGC CCAGCG CAT CAC GGG TTC GCA CCA

[0051] Hairpin 1 (HP1): GCTGGGCCATACGCGGAACGCTATATAGCGTG ATAGCGTTCCGCGTATGGCCCAGCGCATCACG

[0052] Hairpin 2(HP2):CACGCTATATAGCGTTCCGCGTATGGCCCAGCCGTGATGCGCTGG...

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Abstract

The invention relates to a method for detecting the content of bisphenol A by an electrochemical luminescent adaptor sensor. The method comprises the following steps of (a) providing an electrode, wherein DNA capturing single chains are fixed on the surface thereof; contacting the adaptor DNA of the bisphenol A with the electrode to form dsDNA, wherein the CDNA and the adaptor DNA realize partialcomplementary pairing; (b) performing co-incubation on an article to be detected containing bisphenol A with the electrode, so that the bisphenol A and the adaptor DNA are combined; (c) contacting theelectrode with two types of hair clip type DNAHP1 and HP2; forming long chain dsDNA through HCR reaction; marking the long chain dsDNA by an chemiluminescence probe; performing electrochemical luminescence detection, wherein the temperature for the heating step related in the detection process is provided through electrode heating. The method is simple and cheap; no mark exists; high sensitivityis realized; great potential is realized in field investigation aspects of different targets.

Description

technical field [0001] The invention relates to the field of electrochemiluminescence aptasensors, in particular to a method for detecting the content of bisphenol A by using an electrochemiluminescence aptasensor. Background technique [0002] Bisphenol A (BPA), as a key monomer in polymers and related products, is considered to be the most important endocrine disruptor, which can cause harm to human health in low doses [1] . Development of a convenient and inexpensive sensitive assay for BPA could help advance awareness of various health problems associated with endocrine disrupting toxicity [2] . Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) are considered to be the best methods for accurate quantification of BPA, but these methods require complex sample pretreatment processes and take a long time. time [3] . Therefore, in order to save time and simplify operations, related studies have reported methods for detecting...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30C12Q1/68
CPCC12Q1/68G01N27/305G01N27/3276G01N27/3277
Inventor 王培龙苏晓鸥林振宇王瑞国张维程劼
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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