Active NS-GAM gene scaffold for treating scalds and preparation method thereof

A NS-GAM, gene activity technology, applied in pharmaceutical formulations, pharmaceutical science, capsule delivery, etc., can solve problems such as wound infection and difficult to heal, and achieve the effect of alleviating inflammatory response, promoting wound repair, and ideal and benign recovery.

Active Publication Date: 2019-03-29
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Severe cases may even lead to infection of the wound, making it evolve into a deeper wound, which is difficult to heal for a long time
The traditional methods of deep second-degree burn wound repair mostly use external drug therapy and dressing therapy. Years of experience in use have found that the treatment is accompanied by many shortcomings and side effects

Method used

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  • Active NS-GAM gene scaffold for treating scalds and preparation method thereof
  • Active NS-GAM gene scaffold for treating scalds and preparation method thereof
  • Active NS-GAM gene scaffold for treating scalds and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Dissolve NCMC in sterile double-distilled water, stir and dissolve at room temperature, and prepare a solution with a concentration of 3% (w / v). In the same way, a SA solution with a concentration of 3% (w / v) was prepared. The NCMC solution and the SA solution were mixed evenly at a volume ratio of 7:3, and the bubbles in the solution were removed by ultrasonic treatment. Pour the processed mixture carefully into a plate with a diameter of 3 cm, and freeze-dry to obtain a dry solid support. The scaffold was placed in 1% CaCl 2 Carry out the cross-linking reaction in the solution for 12 hours. After the reaction, wash with sterile water several times to remove excess CaCl adsorbed on the scaffold material. 2 solution. Freeze-drying is performed again to obtain a cross-linked and stable NCMC / SA composite scaffold.

[0041] The NCMC / SA support prepared by embodiment 1 is a flat cylinder (such as figure 1 shown), the color is white, the diameter is about 24mm, and the ...

Embodiment 2

[0043] The extract from the NCMC / SA scaffold prepared in Example 1 was prepared according to the national standard ISO 10993-5. Take NIH3T3 cells in the logarithmic growth phase and adjust the density to 4×10 4 cells / ml, inoculated into 96-well plate, at 37℃, 5%CO 2 Incubate for 24 hours under the condition. The original culture medium was discarded, and the culture medium containing the extracts of NCMC / SA scaffolds with different dilutions were added respectively, and the concentrations of the original extracts contained therein were 100%, 50% and 25%, respectively. After culturing for 24 and 48 hours, the cell safety of the scaffold was detected by the MTT method, and the results were as follows: Figure 4 As shown, within the concentration range of each extract, the relative proliferation rate of cells in each group was above 95%, indicating that the NCMC / SA scaffold has no cytotoxicity and has good cytocompatibility. There was no significant difference among different ...

Embodiment 3

[0045] Take NIH3T3 cells in the logarithmic growth phase and adjust the density to 4×10 4 , inoculated onto NCMC / SA scaffolds at 37°C, 5% CO 2Under the conditions, they were cultured with DMEM conventional medium for 24h and 48h respectively. Absorb the culture medium from some samples, centrifuge at 1000rpm for 5 minutes, take the supernatant, and use the lactate dehydrogenase (LDH) kit to measure the activity of lactate dehydrogenase (the specific method is operated according to the instruction manual), the results are as follows Figure 5 shown. At 24h and 48h, compared with the control group, the relative proliferation rates of the cells grown in the scaffold were 94.7% and 100.8%, respectively, indicating that the NCMC / SA scaffold is very helpful for the attachment and growth of cells, which may be related to the NCMC components. Previous studies have shown that NCMC can promote the growth of fibroblasts. The NCMC / SA scaffolds grown with NIH3T3 cells were dehydrated, d...

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Abstract

The invention provides an active NS-GAM gene scaffold for treating scalds. According to the active gene scaffold, a crosslinked scaffold of carboxymethyl chitosan and sodium alginate carries an arginine-chitosan gene vector and the arginine-chitosan gene vector enwraps a target gene. The prepared NS-GAM has an obvious wound repairing effect on scalded skin of a rat, has relatively mild inflammatory reaction, can promote wound repairing by achieving effective in-vivo transfection, has a very significant function of promoting repairing of the skin suffering from deep second-degree scald, and cangenerate a relatively ideal benign repairing effect.

Description

Background technique [0001] Deep second-degree burns are a common clinical disease, and the wound healing process involves the expression of many factors and signal transduction pathways, which is a very complicated process. Although theoretically speaking, the expression of each factor is orderly and cascaded, but in the actual healing process, two types of abnormal healing phenomena, scar hyperplasia or refractory wound, often occur. Severe cases may even lead to infection of the wound surface, making it evolve into a deeper wound surface, which is difficult to heal for a long time. The traditional methods of repairing deep second-degree burns mostly use external drug therapy and dressing therapy. Years of experience in use have found that the treatment is accompanied by many shortcomings and side effects. [0002] In recent years, with the development of gene therapy and the advancement of tissue engineering methods, gene therapy has been gradually applied to the field of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/50A61L27/54A61L27/56
CPCA61L27/20A61L27/50A61L27/54A61L27/56A61L2300/258A61L2300/412A61L2300/62C08L5/08C08L5/04
Inventor 常菁张旺旺陈晓彤张海斌张艳韩宝芹
Owner OCEAN UNIV OF CHINA
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