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Stenotrophomonas sp. HY-2 and application thereof in degradation of organic compounds

A technology of Stenotrophomonas and HY-2, which is applied in the field of high-efficiency new ethyl/butyl acetate degrading bacteria, can solve the problems of undiscovered high-efficiency degradation of ethyl/butyl acetate

Active Publication Date: 2019-03-29
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, through literature search, there is no report on the efficient degradation of ethyl / butyl acetate by Stenotrophomonas using ethyl / butyl acetate as the sole carbon source.

Method used

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  • Stenotrophomonas sp. HY-2 and application thereof in degradation of organic compounds
  • Stenotrophomonas sp. HY-2 and application thereof in degradation of organic compounds
  • Stenotrophomonas sp. HY-2 and application thereof in degradation of organic compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Isolation, purification and identification of Stenotrophomonas HY-2

[0037] (1) Isolation and purification of Stenotrophomonas HY-2

[0038] The activated sludge from the sewage treatment tank of a pharmaceutical factory in Zhejiang was collected on site, and the lower sludge after standing still was mixed with the inorganic salt medium at a ratio of 1:2 (v / v), and the 3L mixture was connected to a 5L sludge acclimation tank (see Jin Xiaojun. Isolation and identification of new dioxane-degrading strains, degradation characteristics and preparation of bacterial agents [D]. Zhejiang University of Technology, 2012.), with ethyl acetate and butyl acetate as substrates as carbon sources and energy sources, at room temperature After nearly 20 days of domestication culture, take 5 mL of sludge from the domestication tank and add it to a shake flask containing 50 mL of inorganic salt medium. It is found that the domesticated sludge can stably degrade 110 mg / L ethyl ...

Embodiment 2

[0047] Embodiment 2: the expanded cultivation of Stenotrophomonas HY-2

[0048] 1) Slant culture: inoculate Stenotrophomonas HY-2 on the LB solid medium of the slant, and culture at 30° C. for 24-36 hours to obtain slant bacteria.

[0049] 2) Expansion culture: use an inoculation loop to pick the slant bacteria obtained in step 1) and inoculate them into LB liquid medium, culture at 30°C for 24-36 hours, and obtain OD 600 =0.1~0.2 bacteria solution.

Embodiment 3

[0050] Embodiment 3: Stenotrophomonas HY-2 degrades the performance of ethyl acetate or butyl acetate

[0051] With ethyl acetate as the sole carbon source of Stenotrophomonas HY-2, get the OD prepared by the method in Example 2 600 =0.1 bacterium liquid, inoculate to fresh 50mL inorganic salt culture medium containing 110mg / L ethyl acetate, make the initial bacterium concentration by OD 600 Calculated as 0.01, pH=7. Put it into a shaker with a temperature of 30°C and a rotation speed of 160r / min for cultivation, take samples every 3 hours to measure the degradation rate of ethyl acetate, and use a 5mL syringe to draw out a part of the bacterial liquid, and measure the OD value of the bacterial cells. The results are shown in image 3 . Under the same conditions, experiment with butyl acetate instead of ethyl acetate, the results are shown in Figure 4 . During the experiment, two parallel samples and a blank control group not inoculated with strains were designed. With t...

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Abstract

The invention discloses a new bacterial strain namely stenotrophomonas sp. (Stenotrophomonas sp.) HY-2 which is preserved in China Center for Type Culture Collection, the address of the China Center for Type Culture Collection is Wuhan University, Wuhan 430072, China, the preservation data is 26th, October, 2018, and the preservation number is CCTCC No: M 2018714. The bacterial strain disclosed bythe invention can grow and propagate by using organic compounds of ethyl acetate, butyl acetate and the like as a sole carbon source and energy, and substrates are mineralized into CO2 and H2O; underthe pure cultivation condition, the bacterium has better degradation capacity on the ethyl acetate and the butyl acetate when the pH is 6.0-8.0 and the temperature is in the range of 25-35 DEG C; andthe bacterial strain has high adaptive capacity to environment, and provides powerful guarantee for the engineering application of purification of ethyl acetate / butyl acetate exhaust gas and ethyl acetate / butyl acetate waste water by a biological method.

Description

(1) Technical field [0001] The invention relates to a strain of high-efficiency novel ethyl / butyl acetate degrading bacteria—Stenotrophomonas sp. HY-2 and its application in microbial degradation of ethyl / butyl acetate. (2) Background technology [0002] Ethyl acetate is a colorless, transparent liquid with a pungent odor. It has excellent solubility and quick drying. It is a fine chemical product with a wide range of uses. It is widely used in the production process of acetate fiber, vinyl resin, and synthetic rubber. . However, ethyl acetate is irritating to the eyes, nose, and throat. Inhalation of high concentrations can cause anesthesia, acute pulmonary edema, liver and kidney damage. Sustained inhalation of large amounts may cause respiratory paralysis. Those who take it by mistake can produce nausea, vomiting, abdominal pain, diarrhea and so on. Long-term exposure to this product can sometimes cause corneal opacity, secondary anemia, and leukocytosis. [0003] Bu...

Claims

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Application Information

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IPC IPC(8): C12N1/20B01D53/84B01D53/72C12R1/01
CPCB01D53/72B01D53/84B01D2251/95C12N1/20C12N1/205C12R2001/01Y02P20/59Y02A50/20
Inventor 陈东之陈建孟刘浩阳叶杰旭成卓韦张士汉
Owner ZHEJIANG UNIV OF TECH
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