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Compound preparation prepared from protease and protein-degrading bacteria, and application thereof to cigars

A technology of protein degradation and preparation, applied in the manufacture of cigars, applications, bacteria, etc., can solve the problems of not being able to effectively improve the quality of cigar tobacco leaves, and the reduction effect is not obvious enough, so as to achieve good practical value, promotion and application significance, and quality improvement. Effect

Active Publication Date: 2019-04-02
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the actual test and application results also show that: at the initial stage of protease application, the effect of reducing protein content in cigar tobacco leaves is more obvious, but with the prolongation of time and the decrease of enzyme activity, the effect of reducing protein content also declines rapidly. Not obvious enough to effectively improve the quality of cigar leaves

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The specific preparation method of the compound preparation of protease and protein-degrading bacteria provided in this application is as follows.

[0039] (1) Prepare protein-degrading bacteria liquid, specifically:

[0040] Prepare OD with Bacillus pumilus (Bacillus pumilus) SMXP-03 strain 600 =1.0 or so bacteria liquid for later use; the specific preparation process can be referred to as follows:

[0041] First, mix flue-cured tobacco powder (passed through a 200-mesh sieve after crushing) and sodium chloride evenly, add water (per 100mL of water, add 5g of tobacco powder, 0.8g of sodium chloride), stir and mix evenly, 121 ℃, Sterilize for 15 minutes, cool to room temperature and use as culture medium;

[0042] Secondly, after inoculating the culture medium with Bacillus pumilus SMXP-03 strain, cultured with shaking at 37°C and 120r / min for 24 hours, collected the precipitate by centrifugation, resuspended the precipitate with sterile water, and adjusted the OD 60...

Embodiment 2

[0050] Using the compound preparations prepared in Example 1 in different proportions, the inventors conducted experiments on reducing the protein content of cigar leaves. The specific process is briefly described as follows.

[0051] First, the cigar tobacco leaves are subjected to moisture resurgence treatment, and the moisture content is adjusted to about 15%, and then balanced for 24 hours after the moisture resurgence treatment;

[0052] Secondly, according to the 2% mass ratio of cigar tobacco leaves, the compound preparation prepared in different proportions in Example 1 is evenly sprayed on the surface of cigar tobacco leaves, adjust and control the moisture content of tobacco leaves to about 20%, and let it stand to make its moisture balance;

[0053] Then stack and place the tobacco leaves, control the pile temperature at about 37°C, and the ambient humidity at about 75%, pile up and ferment, during which the pile is turned and loosened every 1 day to ensure uniform f...

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PUM

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Abstract

The invention belongs to the technical field of cigar preparation, and particularly relates to patent applications of a compound preparation prepared by using protease and protein-degrading bacteria,and an application thereof to cigars. The preparation process of the compound preparation comprises the following steps: preparing a protein-degrading bacterium liquid by using bacillus pumilus SMXP-03, preparing an alkaline protease solution for later use, and preparing the compound preparation. The application designs the fermentation alcoholization of cigar tobacco, and realizes the degradationof nitrogenous substances, especially protein components in the cigar tobacco by using the compound preparation prepared from alkaline protease and specific protein-degrading bacterium strains. The preliminary test and application results show that after treatment, the quality of the cigar tobacco is improved, and the purpose of improving the quality and reducing consumption is better achieved. At the same time, the preparation has a good promoting effect on the production of cigars, so that the preparation has better practical value and promotion application significance.

Description

technical field [0001] The invention belongs to the technical field of cigar tobacco preparation, and specifically relates to a compound preparation prepared by using protease and protein-degrading bacteria and its application in cigars. Background technique [0002] Nitrogen-containing substances, especially protein, are one of the main components in tobacco leaves. A certain amount of protein is conducive to increasing the satisfaction of tobacco smoking and providing rich aroma, but too high protein content will lead to obvious smoke during the burning process of tobacco leaves. The smell of burnt feathers makes it have a distinct bitterness when inhaled, and it also increases the release of some harmful substances. Therefore, the nitrogen-containing substances in tobacco leaves, especially the protein content must be properly adjusted and controlled in order to better ensure the smoking quality of tobacco leaves. [0003] As far as the improvement of tobacco leaf qualit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/50A24B3/04A24B15/20A24C1/00C12R1/07
CPCA24B3/04A24B15/20A24C1/00C12N1/20C12N9/50
Inventor 张婷婷冯颖杰邱建华张展杨宗灿郝辉楚文娟
Owner CHINA TOBACCO HENAN IND
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