Clostridium perfringens type B exotoxin, preparation method thereof, toxigenic culture medium and application of clostridium perfringens type B exotoxin

Abstract

The invention discloses a clostridium perfringens type B exotoxin, a preparation method thereof, a toxigenic culture medium and an application thereof. Each 1000 ml of the toxigenic culture medium isprepared from the following raw materials by weight: 15-25 g of peptone, 3-5 g of yeast extract, 3-4 g of NaCl, 5-10 g of Na2HPO4.12H2O, 5-10 g of dextrin or 5-10 g of coarse dextrin, and the balanceof water for injection. The virulence of the clostridium perfringens toxin liquid produced by the method according to the present invention is up to 1 mouse MLD of no more than 0.0006 mL, and the virulence is 3.3 times than the vaccine production standard. Moreover, a triple inactivated vaccine against bradsot, struck (or lamb dysentery) and enterotoxemia prepared by the clostridium perfringens type B exotoxin has serum neutralizing titer in rabbits and sheep equal to or higher than the relevant standard.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, and in particular relates to a type B Clostridium perfringens exotoxin, a preparation method thereof, a toxin-producing medium and applications thereof. Background technique [0002] Clostridium perfringens is a general term for diseases caused by pathogenic species of Clostridium. Clostridium is a class of anaerobic bacteria, there are more than 60 species, and only more than 10 common pathogenic bacteria, mostly present in soil, sewage, and human and various animal feces. Gram staining was positive, except for a few bacterial species, all had flagella, which could move and form spores, and their diameter was larger than that of bacteria. Most bacterial species can produce exotoxins with severe toxicity, which are not only the main factor of pathogenicity, but also the main antigen. After being transformed into toxoids, they can stimulate animals to produce antitoxins, which can be...

AI Technical Summary

Problems solved by technology

[0005] At present, the vaccines used in the industry generally use animal raw materials such as beef, fish, and beef liver to prepare anaerobic meat liver and stomach enzyme digestion soup and cod liver and stomach enzyme digestion soup medium, but using this method to prepare the medium is often due to raw materials. The quality is uneven, the difference between batches is large, and the phenomenon of unstable toxin production performance often occurs. Especially in recent years, due to the development of the domestic dairy industry, the number of dairy cows has increased rapidly, and a large number of eliminated dairy beef has flooded the market. The quality of the raw materials used to prepare the vaccine medium has declined; at the same time, the residual drugs in beef and beef liver often inhibit the growth of bacteria. decline in vaccine quality

In addition, the traditional medium preparation process is cumbersome, time-consuming, requires a lot of manpower, high price, and low recovery rate, which also brings great waste and high cost to vaccine production, causing great troubles to vaccine manufacturers

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