A kind of handmade soap containing nasturtium stem cell extract and preparation method thereof
A nasturtium and stem cell technology, applied in the field of daily chemical products, can solve the problems of shortening the shelf life of handmade soap, ineffectiveness, and prone to corruption, etc., and achieves the effects of relieving skin oxidative damage, preventing bacterial infestation, and relieving skin itching.
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Embodiment 1
[0036] Embodiment 1, the preparation method of nasturtium stem cell extract
[0037] A get the root tip of Trollius japonica with root cap removed, clean and disinfect, cut the root tip of Trollius chinensis into 5mm*5mm size to obtain explants;
[0038]B Put the explants obtained in step A into the induction medium for culture, the induction medium is: MS medium+BA2mg / L+NAA 2mg / L, the pH value is 5.5~6.0, and the culture temperature is 25±2 Cultivate for 5 days under the condition of 14h / d light to obtain quiescent central stem cells;
[0039] C. Transfer the quiescent central stem cells obtained in step B to a growth medium for shaking culture. The growth medium is: MS medium+BA2mg / L+NAA2.5mg / L+potato juice 10%, pH 5.2-5.6, The culture temperature is 23-25°C, the light intensity is 2000lx, and the light time is 10h / d to obtain single cells;
[0040] D expand the single cells obtained in step C, and the culture medium for the expansion is: MS medium+BA1mg / L+NAA2mg / L+potato ...
Embodiment 2
[0041] Embodiment 2, the preparation method of nasturtium stem cell extract
[0042] A get the root tip of Trollius japonica with root cap removed, clean and disinfect, cut the root tip of Trollius chinensis into 5mm*5mm size to obtain explants;
[0043] B Put the explants obtained in step A into the induction medium for culture, the induction medium is: MS medium+BA3mg / L+NAA 3mg / L, the pH value is 5.5~6.0, and the culture temperature is 25±2 Cultivate for 5 days under the condition of 14h / d light to obtain quiescent central stem cells;
[0044] C. Transfer the quiescent central stem cells obtained in step B to a growth medium for shaking culture. The growth medium is: MS medium+BA 3mg / L+NAA 3mg / L+potato juice 12%, pH 5.2-5.6, The culture temperature is 23-25°C, the light intensity is 2000lx, and the light time is 10h / d to obtain single cells;
[0045] D The single cells obtained in step C are expanded and cultivated. The medium for the expanded culture is: MS medium+BA1.5mg...
Embodiment 3
[0046] The preparation method of embodiment 3, nasturtium essential oil:
[0047] Accurately weigh 25g of nasturtium flower powder, put it in a 500mL round bottom flask, add 300mL of distilled water, add zeolite, connect to one end of the simultaneous distillation extraction device, heat, and connect the other end of the device to a 100mL flask containing 40mL of dichloromethane, water bath Heated to 100°C, distilled and extracted for 6h, dried with anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure to obtain that product.
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