Method for detecting residual amount of Florfenicol in pig hair
A florfenicol and detection method technology, applied in the field of qualitative and quantitative detection of florfenicol drug residues in pig hair, achieving the effect of low detection limit, quantitative limit and high recovery rate
Active Publication Date: 2019-04-05
INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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Problems solved by technology
These methods are mostly used in the determination of florfenicol drug in animal tissue, but the detection method of florfenicol drug residue in pig hair has no report
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[0042] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the methods described in the examples of the present invention are only used to illustrate the present invention, rather than to limit the present invention, and under the premise of the concept of the present invention, simple improvements to the preparation method of the present invention all belong to the scope of protection of the present invention.
[0043] 1. Configuration of standard solution:
[0044] Standard stock solution: Weigh an appropriate amount of florfenicol standard substance, prepare 1.0mg / mL standard stock solution with methanol, and store at -18°C in the dark.
[0045]Standard intermediate solution: take 1mL of the above standard stock solution, dilute it with methanol to a standard intermediate solution with a mass concentration of 10.0mg / L, and store it at -18°C in the dark.
[0046] Standard ...
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Abstract
The invention discloses a method for detecting a residual amount of Florfenicol in pig hair. The method comprises the following steps of: (1) a pretreatment of a pig hair sample: carrying cleaning, alkali hydrolysis, and organic solvent extraction on the pig hair sample; and (2) using a Florfenicol-D3 standard working fluid as internal standard, and detecting the residual amount of the Florfenicolin the pig hair by superhigh performance liquid chromatography-electrospray tandem mass spectrometry. According to the method for detecting the residual amount of the Florfenicol in the pig hair, animals such as live pigs do not need to be slaughtered to realize living body detection of animals; whether the Florfenicol is used in the growth process of the livestock and poultry can be accurately monitored since the hair growth period is long and the drug metabolism is slow; and the detection steps are easy and convenient to operate, and the detection result is accurate and reliable.
Description
technical field [0001] The invention belongs to the field of drug residue detection, in particular to a method for qualitatively and quantitatively detecting florfenicol drug residue in pig hair. Background technique [0002] Florfenicol, also known as fluthiamphenicol, is a fluorinated derivative of chloramphenicol. Its molecular structure has no potential aplastic anemia-causing nitro group, and it has a broad antibacterial spectrum and is easy to absorb. Therefore, florfenicol has become the main substitute of chloramphenicol and is widely used in the treatment of gram-positive bacteria, gram-negative bacteria and chloramphenicol-resistant bacteria in animals. However, the reproductive toxicity test results show that Florfenicol has blood toxicity, embryotoxicity and immunosuppressive effects, and adverse reactions such as anorexia and diarrhea will also occur after medication. Eating a large amount of pork with excessive Florfenicol will bring harm to human health. Grea...
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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/7266G01N2030/027
Inventor 胡丽芳范芳赖艳李伟红熊艳
Owner INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI