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Fermentation process for increasing 2-keto-D-gluconic acid generation velocity of strain

A technology of gluconic acid and fermentation process, applied in the directions of fermentation, microorganism-based methods, microorganisms, etc., can solve problems such as incomprehension, and achieve the effects of high conversion rate, shortened fermentation cycle, and reduced production cost

Active Publication Date: 2019-04-09
QILU UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on 2-KGA is mainly focused on improving the conversion rate and genetic engineering transformation. The research on shortening the fermentation cycle and improving production efficiency on the basis of maintaining a high conversion rate is not thorough, and needs to be further improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A fermentation process for improving the rate of producing 2-keto-D-gluconic acid by bacterial strains, comprising the steps of:

[0044](1) Strain selection: Serratia marcescens (Serratia marcescens) SDSPY-623 was selected;

[0045] (2) Slant surface culture activation: inoculate the bacteria on the slant medium, and culture it statically for 20 hours at 28~32°C, and set it aside;

[0046] (3) Seed cultivation: Put the strain cultivated in step (2) under aseptic conditions into a 50 mL (500 mL Erlenmeyer flask) liquid seed medium with an inoculation loop, set the rotation speed to 200 rpm, and rotate On a shaker with a radius of 40mm, cultivate at 37°C for 8 hours to obtain a seed solution;

[0047] (4) Fermentation culture:

[0048] Shake flask fermentation: Inoculate the seed liquid into a shake flask containing 50 mL (500 mL Erlenmeyer flask) fermentation medium with an inoculation amount of 10% by volume, and control the temperature at 37°C for the first 8 hours,...

Embodiment 2

[0068] A fermentation process for improving the rate of producing 2-keto-D-gluconic acid by bacterial strains, comprising the steps of:

[0069] (1) Strain selection: Serratia marcescens (Serratia marcescens) SDSPY-623 was selected;

[0070] (2) Slant surface culture activation: inoculate the bacteria on the slant medium, and culture it statically for 21 hours at 29°C, and set it aside;

[0071] (3) Seed culture: Put the strain cultivated in step (2) under sterile conditions into 53 mL (500 mL Erlenmeyer flask) liquid seed medium with an inoculation loop, set the rotation speed to 200 rpm, and rotate On a shaker with a radius of 40mm, culture at 37°C for 9 hours to obtain a seed liquid;

[0072] (4) Fermentation culture:

[0073] Fermentation in a 10L fermenter: Inoculate the seed solution in a 10L fermenter with 7L of fermentation medium at an inoculum volume of 10% by volume, and control the temperature at 37°C for the first 8 hours, with a rotation speed of 300 rpm and an...

Embodiment 3

[0092] A fermentation process for improving the rate of producing 2-keto-D-gluconic acid by bacterial strains, comprising the steps of:

[0093] (1) Strain selection: Serratia marcescens (Serratia marcescens) SDSPY-623 was selected;

[0094] (2) Slant surface culture activation: inoculate the bacteria on the slant medium, and culture it statically for 20 hours at 37°C, and set it aside;

[0095] (3) Seed culture: Put the strain cultivated in step (2) under sterile conditions into 50 mL (500 mL Erlenmeyer flask) liquid seed medium with an inoculation loop, set the rotation speed to 220 rpm, and rotate On a shaker with a radius of 40mm, cultivate at 37°C for 8.5 hours to obtain a seed solution;

[0096] (4) Fermentation culture:

[0097] Fed-batch fermentation: Inoculate the seed solution in a 10L fermenter with 4L fermentation medium at an inoculum volume of 9%, control the temperature at 37°C for the first 8 hours, rotate at 300 rpm, and ventilate The amount is 0.6Nm 3 / h,...

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Abstract

The invention discloses a fermentation process for increasing the 2-keto-D-gluconic acid generation velocity of a strain and belongs to the field of fermentation process. According to the fermentationprocess, a serratia marcescens SDSPY-623 strain is subjected to strain inclined surface activation, seed culture and fermentation culture in sequence to obtain a 2-KGA containing fermentation liquid,and a fermentation culture medium is prepared from glucose, corn steep liquor, monopotassium phosphate, anhydrous magnesium sulfate, ferrous sulfate, cobalt chloride, ammonium molybdate, boric acid,zinc sulfate, nicotinic acid, calcium carbonate and tap water of which the pH value is 6.5-7.2. By adjusting fermentation culture mediums, fermentation temperatures and pH values and setting sectionalfermentation modes in fermentation culture steps, the conversion rate can be increased, meanwhile, the fermentation cycle is remarkably shortened, the production cost can be reduced, the production efficiency of 2-KGA is improved by 32% or greater when being compared with that of a conventional fermentation process, and the fermentation process has great industrial production significances for abiological fermentation method of 2-KGA.

Description

technical field [0001] The invention relates to the field of fermentation engineering, in particular to a fermentation process for improving the rate of producing 2-keto-D-gluconic acid by bacterial strains. Background technique [0002] 2-keto-D-gluconic acid (2-KGA) is a food additive (antioxidant) D-erythorbic acid (EA) and its sodium salt (Sodium erythorbate, EN) The key intermediate in the industrial production process, and it also has a wide range of uses. It can be used as calcium-rich additives for feed, detergent synergists, cement plasticizers and photographic imaging developers; as synthetic amino sugars, deoxy sugars and The intermediate of aldonic acid; as an important raw material for the biosynthesis of aldose, ribulose and hydroxymalonic acid, etc. [0003] The synthesis methods of 2-KGA mainly include chemical synthesis, enzymatic and biological fermentation. The chemical synthesis method is based on Pt / Pb as catalyst, with O 2 A method for oxidizing gluc...

Claims

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Application Information

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IPC IPC(8): C12P7/60C12R1/43
CPCC12P7/60
Inventor 徐慧李文婧张俊娇刘建军田延军
Owner QILU UNIV OF TECH
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