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Growth factor sustained-release microsphere and preparation method thereof

A growth factor and slow-release microsphere technology, applied in the field of medicine, can solve the problems of ineffectiveness, short half-life, protease hydrolysis, etc., and achieve the effects of reducing the occurrence of cytotoxicity, maintaining activity, and enhancing the slow-release ability

Active Publication Date: 2019-04-12
GUANGZHOU RAINHOME PHARM&TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, TGF-β is an important biological mediator in the process of injury response, TGF-β1 is one of the important subtypes of TGF-β, in the process of embryonic cartilage formation, TGF-β1 can induce primitive mesenchymal stem cells to differentiate into cartilage tissue, and has the effect of promoting the proliferation and maturation of chondrocytes, but the cytokines are not stable, have a short half-life in vivo, are easily hydrolyzed by proteases, and cannot act on more target cells within an effective time

Method used

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  • Growth factor sustained-release microsphere and preparation method thereof
  • Growth factor sustained-release microsphere and preparation method thereof
  • Growth factor sustained-release microsphere and preparation method thereof

Examples

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Embodiment 1

[0032] An embodiment of the growth factor slow-release microspheres of the present invention, the growth factor slow-release microspheres of the present embodiment include the following components by weight:

[0033] (a) 3 parts of sulfonated chitosan, 4 parts of heparin, 0.005 part of growth factor, 8 parts of polyamino acid, 5 parts of polyanhydride, 7 parts of thickener, and 5 parts of insoluble polymer carrier;

[0034] (b) 3.5 parts of natural crosslinking agent.

[0035] Wherein, the growth factor is TGF-β1, the thickener is carboxymethyl cellulose, the insoluble polymer carrier is polylactic acid-glycolic acid copolymer, and the natural crosslinking agent is tannic acid .

[0036] The preparation method of the growth factor sustained-release microspheres of the present embodiment is:

[0037] 1) Dissolve 3 mg of sulfonated chitosan and 4 mg of heparin in 10 mL of purified water to obtain mixed solution A, then add 5 μg of TGF-β1 growth factor to mixed solution A and m...

Embodiment 2

[0044] An embodiment of the growth factor slow-release microspheres of the present invention, the growth factor slow-release microspheres of the present embodiment include the following components by weight:

[0045] (a) 2 parts of sulfonated chitosan, 1 part of heparin, 0.001 part of growth factor, 1 part of polyamino acid, 3 parts of polyanhydride, 1 part of thickener, and 4 parts of insoluble polymer carrier;

[0046] (b) 2 parts of natural crosslinking agent.

[0047] Wherein, the growth factor is TGF-β1, the thickener is carboxymethyl cellulose, the insoluble polymer carrier is polylactic acid-glycolic acid copolymer, and the natural crosslinking agent is tannic acid .

[0048] The preparation method of the growth factor sustained-release microspheres of the present embodiment is:

[0049] 1) Dissolve 2 mg of sulfonated chitosan and 1 mg of heparin in 10 mL of purified water to obtain mixed solution A, then add 1 μg of TGF-β1 growth factor to mixed solution A and mix to...

Embodiment 3

[0056] An embodiment of the growth factor slow-release microspheres of the present invention, the growth factor slow-release microspheres of the present embodiment include the following components by weight:

[0057] (a) 5 parts of sulfonated chitosan, 6 parts of heparin, 0.01 part of growth factor, 15 parts of polyamino acid, 10 parts of polyanhydride, 10 parts of thickener, and 9 parts of insoluble polymer carrier;

[0058] (b) 5 parts of natural crosslinking agent.

[0059] Wherein, the growth factor is TGF-β1, the thickener is carboxymethyl cellulose, the insoluble polymer carrier is polylactic acid-glycolic acid copolymer, and the natural crosslinking agent is tannic acid .

[0060] The preparation method of the growth factor sustained-release microspheres of the present embodiment is:

[0061] 1) Dissolve 5 mg of sulfonated chitosan and 6 mg of heparin in 10 mL of purified water to obtain a mixed solution A, then add 10 μg of TGF-β1 growth factor to the mixed solution ...

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Abstract

The invention relates to a growth factor sustained-release microsphere and a preparation method thereof, and belongs to the technical field of medicine. The growth factor sustained-release microsphereof the present invention comprises the following components in parts by weight: (a) 2-5 parts of sulfonated chitosan, 1-6 parts of heparin, 0.001-0.01 part of growth factor, 1-15 parts of polyamino acid, 3-10 parts of polyanhydride, 1-10 parts of a thickener and 4-9 parts of a water-insoluble polymer carrier; and (b) natural crosslinking agents. The method combines the sulfonated chitosan with the heparin, and can enhance the sustained-release ability of the growth factor sustained-release microsphere on the growth factor and maintain growth factor activity. Studies show that the growth factor sustained-release microsphere of the present invention has a high drug entrapment rate and can stably release medicines, and the medicine release lasts for a long time.

Description

technical field [0001] The invention relates to a growth factor sustained-release microsphere and a preparation method thereof, belonging to the technical field of medicine. Background technique [0002] Growth factors are active proteins or polypeptides that exist in organisms and have extensive regulatory effects on the growth and development of organisms. They control many key cell activities in the process of tissue repair. Among them, TGF-β is an important biological mediator in the process of injury response, TGF-β1 is one of the important subtypes of TGF-β, in the process of embryonic cartilage formation, TGF-β1 can induce primitive mesenchymal stem cells to differentiate into cartilage tissue, and has the effect of promoting the proliferation and maturation of chondrocytes, but the cytokines are not stable, have a short half-life in vivo, are easily hydrolyzed by proteases, and cannot act on more target cells within an effective time. Contents of the invention [...

Claims

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Application Information

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IPC IPC(8): A61K9/52A61K38/18A61K47/36A61P19/08
CPCA61K9/5036A61K38/1841A61P19/08
Inventor 黄燕飞刘少辉车七石
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
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