Oligonucleotide synthesis chip system and use method thereof

A technology of oligonucleotide and chip system, which is applied in the field of solid-phase chemical synthesis chip system, can solve the problems of reducing synthesis cost and chip manufacturing cost, and achieve the effects of reducing synthesis cost, low manufacturing cost and simple processing technology

Active Publication Date: 2019-04-12
杭州原合生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The processing technology of the oligonucleotide synthesis chip system of the present invention is simple, does not require complex surface modification, and the chip manufacturing cost is low. At the same time, the synthesis carrier of the oligonucleotide can be loaded multiple times according to the needs, thereby significantly reducing the synthesis cost, and can Solve technical problems that have long been wanted to be solved in this field but have not been solved

Method used

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  • Oligonucleotide synthesis chip system and use method thereof
  • Oligonucleotide synthesis chip system and use method thereof
  • Oligonucleotide synthesis chip system and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1 becomes the preparation of oligonucleotide synthesis chip

[0056] Please see attached figure 1 And attached figure 2, in this embodiment, the prepared oligonucleotide synthesis chip 101 has a length of 127 mm and a width of 85 mm, which is the same as that of a common 96-well plate. The diameter of the upper opening 201 of the micro-funnel structure 103 in the prepared oligonucleotide synthesis chip 101 is 0.15 mm, and the depth is 0.20 mm. The diameter of the lower end opening 202 is 0.02 mm, and the depth is 0.30 mm. The micro-funnel structure 103 The spacing is 0.3 mm, and the number of micro-funnel structures 103 in each group of micro-funnel structures 102 is 10×10, that is, 100. The distance between each group of micro funnel structures 102 is 3 mm.

[0057] Please see attached Figure 4 , in this embodiment, the prepared oligonucleotide synthesis chip 101 is made of silicon material, which is prepared by dry etching twice. Firstly, the photore...

Embodiment 2

[0059] Example 2 Synthetic Carrier Particle Size Screening Chip Manufacturing

[0060] Please see attached image 3 , in this embodiment, the prepared synthetic carrier particle size screening chip 301 has an appearance size of 127 mm in length and 85 mm in width, which is the same as that of a common 96-well plate. The diameter of the upper opening 303 of the sieve structure 302 in the prepared synthetic carrier particle size screening chip 301 is 0.14 mm, the depth is 0.20 mm, the diameter of the lower end opening 304 is 0.13 mm, and the depth is 0.30 mm. is 0.3 mm.

[0061] Please see attached Figure 4 , in this embodiment, the prepared synthetic carrier particle size screening chip 301 is made of silicon material, which is prepared by dry etching twice. As shown in step 403, first, the photoresist 402 is spin-coated on the front surface of the silicon wafer 401, and the micropattern to be etched on the upper surface of the synthetic carrier particle size screening chip...

Embodiment 3

[0063] Embodiment 3 Synthetic carrier sorting method

[0064] The basic material of commercially available DNA synthesis carriers is porous glass, which is usually used to fill the synthesis columns used in commercial synthesizers. The particle size distribution range is wide, usually reaching about 0.12-0.18 mm. Compared with the synthetic carrier with larger particle size, the difference in the synthetic load of DNA can reach more than 3 times. For high-throughput oligonucleotide synthesis, the initial synthesis load varies too much, which can lead to significant differences in the yield of the target product. However, high-throughput oligonucleotide synthesis is usually used to construct high-throughput DNA libraries, perform long-sequence DNA or gene splicing, etc. If the yield of target products is too different, it will lead to poor DNA library uniformity and failure of long-sequence DNA splicing. Therefore, before applying the oligonucleotide synthesis reaction microch...

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Abstract

The invention discloses an oligonucleotide synthesis chip system. The oligonucleotide synthesis chip system comprises an oligonucleotide synthesis chip and a synthetic carrier particle size screeningchip, wherein the high-throughput oligonucleotide synthesis chip comprises a micro-funnel structure with two ends open, an upper end opening is located in the upper surface of the high-throughput oligonucleotide synthesis chip, a lower end opening is located in the lower surface of the high-throughput oligonucleotide synthesis chip, and the caliber of the upper end opening is larger than that of the lower end opening. The synthetic carrier particle size screening chip comprises screen holes, wherein the upper surface opening diameter of the screen holes is smaller than the upper end opening aperture of the oligonucleotide synthesis chip, the lower surface opening diameter of the screen holes is obvious larger than the lower end opening diameter of the oligonucleotide synthesis chip, and the lower surface opening diameter of the screen holes is slightly smaller than the upper surface opening diameter of the screen holes.

Description

technical field [0001] The invention relates to the field of microfluidic technology, and also relates to the field of solid-phase DNA chemical synthesis. More specifically, the present invention relates to a solid-phase chemical synthesis chip system for high-throughput oligonucleotides using a microfluidic chip and a method for using the same. Background technique [0002] DNA is the most critical biomacromolecule, and the preparation of DNA according to needs is of great significance in scientific research and application fields. Although biological methods such as microbial culture and reproduction can be used to obtain DNA in large quantities, and DNA can also be amplified by polymerase chain reaction (PCR amplification), both require templates to prepare DNA from scratch without templates, and chemical synthesis methods must still be used. Solid-phase chemical synthesis is the main method for chemically synthesizing DNA. Currently, the solid-phase DNA solid-phase synt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J19/00
CPCB01J19/0046B01J19/0093
Inventor 郑晖蔡万世
Owner 杭州原合生物科技有限公司
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