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Method of utilizing cordyceps militaris to inoculate castor silkworm 5-year larvae to cultivate silkworm cordyceps

A technology of castor silkworm and Cordyceps militaris, which is applied in the field of Cordyceps cultivation, can solve the problem of less Cordyceps militaris, and achieve the effects of preventing strain degradation, large individuals, and improving vitality and infection ability

Active Publication Date: 2019-04-19
湖南省蚕桑科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Castor silkworm Cordyceps militaris is a kind of Cordyceps militaris, and there are few reports on successfully artificially cultivated Cordyceps militaris related to castor silkworm

Method used

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  • Method of utilizing cordyceps militaris to inoculate castor silkworm 5-year larvae to cultivate silkworm cordyceps

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Embodiment 1

[0033] This embodiment includes the following steps:

[0034] (1) Host selection of Cordyceps militaris: Select healthy silkworm bodies of the fifth instar 3d castor silkworm. After the 5th instar is fed, the castor leaves are continuously fed at a temperature of 25°C and a relative humidity of 60%, ensuring ventilation and timely detoxification. Sand, select robust castor silkworm larvae for subsequent use;

[0035] (2) Expanded cultivation of strain liquid preparation solution: select a first-generation hybrid variety with strong disease resistance, silkworm larvae with strong infectivity, stable herb production, high-quality and high-yield fruiting bodies of Cordyceps militaris slanted mother species, and 1 piece of soybean-sized pupa Cordyceps slanted mother species were inoculated on 1L of ready-made solid plate medium added with 5wt% silkworm chrysalis powder. After 10 days of activation, under aseptic operation, the strains were evenly divided into soybean-sized strains...

Embodiment 2

[0045] This embodiment includes the following steps:

[0046] (1) Host selection of Cordyceps militaris: Select the healthy castor-oil silkworm body of the fifth instar 4d. After the 5th instar is fed, the temperature is 28°C and the relative humidity is 65%, and the castor leaves are continuously fed to ensure ventilation and timely removal. Sand, select robust castor silkworm larvae for subsequent use;

[0047] (2) Expanded cultivation of strain liquid preparation solution: select a first-generation hybrid variety with strong disease resistance, silkworm larvae with strong infectivity, stable herb production, high-quality and high-yield fruiting bodies of Cordyceps militaris slanted mother species, and 1 piece of soybean-sized pupa Cordyceps slanted mother species were inoculated on 1L of ready-made solid plate medium added with 7wt% silkworm chrysalis powder. After 9 days of activation, under aseptic operation, the strains were evenly divided into soybean-sized strains, and...

Embodiment 3

[0057] This embodiment includes the following steps:

[0058] (1) Selection of the host of Cordyceps militaris; select healthy silkworm bodies of the fifth instar 5d castor silkworm. After the 5th instar is fed, the castor leaves are continuously fed at a temperature of 30°C and a relative humidity of 70% to ensure ventilation and timely sand removal. , select robust castor silkworm larvae for subsequent use;

[0059] (2) Expand the cultivation of the liquid preparation solution of the strain, select a first-generation hybrid variety with strong disease resistance, silkworm larvae with strong infectivity, stable weeding, high-quality and high-yield fruiting bodies of Cordyceps militaris slant female species, and 1 piece of soybean-sized pupa Cordyceps slanted mother species were inoculated on 1L of ready-made solid plate medium added with 8wt% silkworm chrysalis powder. After 7 days of activation, under aseptic operation, the strains were evenly divided into soybean-sized stra...

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Abstract

A method of utilizing cordyceps militaris to inoculate castor silkworm 5-year larvae to cultivate silkworm cordyceps includes following steps: (1), selecting a cordyceps militaris host; (2), performing enlarged culture on a strain liquid preparation solution; (3), treating host castor silkworm after inoculating; (4), performing cultivation management on silkworm cordyceps fruiting bodies: inoculating a cordyceps militaris bevel mother strain onto a solid flat plate culture medium for activation, inoculating into a liquid culture medium, inoculating into healthy castor silkworm larvae after enlarged culture for infection ossification, and putting infected and ossified silkworm without bacteria spawning into a transparent plastic box after going through sterile treatment for culture management to obtain silkworm cordyceps. The advantage that castor silkworm larvae are big in size, soft in body and rich in nutrition is fully utilized to cultivate the fruiting bodies; infectious bacteria contamination rate is low, infection ossification rate and grass emerging rate are high, and obtained castor silkworm cordyceps is high in stability and fruiting body yield, rich in active ingredient content, suitable for large-scale production and wide in development prospect.

Description

technical field [0001] The invention relates to a method for cultivating Cordyceps silkworm by using Cordyceps militaris to inoculate 5th instar larvae of castor silkworms, and belongs to the technical field of Cordyceps cultivation. Background technique [0002] Cordyceps is a kind of medicinal and edible fungus with remarkable nourishing effect, and has the reputation of "living gold". As the old saying goes: "It is better to have a handful of Cordyceps than a cart full of gold and jade". Cordyceps sinensis, Cordyceps militaris and Cicada are all the best of Chinese medicinal Cordyceps. Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, is a model species of the genus Cordyceps in the family Ergotaceae. A complex of fungi and insect shells that coexist. The medicinal value of Cordyceps militaris has been recorded in many medical bibliographies. Its medicinal properties are mild, and it has a wide range of medicinal and edible properties withou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/50A01G18/20A01G18/60A01G18/69A01G18/30
CPCA01G18/20A01G18/30A01G18/50A01G18/60A01G18/69
Inventor 张俊李一平颜新培廖模祥钱荷英张月华李刚张晓米雅兰徐瑛雷语
Owner 湖南省蚕桑科学研究所
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