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Quantitative detection method of drug resistance gene in Taihu Lake water body and sediment

A quantitative detection method and technology of drug resistance genes, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, special purpose ships, etc. high efficiency

Inactive Publication Date: 2019-04-19
NANJING NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are a lot of studies on resistance genes in areas with high antibiotic content such as sewage treatment plants, aquaculture environments, and livestock breeding environments, but there are few studies on resistance genes in natural water bodies and sediments

Method used

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  • Quantitative detection method of drug resistance gene in Taihu Lake water body and sediment
  • Quantitative detection method of drug resistance gene in Taihu Lake water body and sediment
  • Quantitative detection method of drug resistance gene in Taihu Lake water body and sediment

Examples

Experimental program
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Embodiment Construction

[0049] This example specifically illustrates the method for the quantitative detection of the resistance genes sul2 and tetO in the water body and sediment of Taihu Lake.

[0050] Step 1: Collection methods of natural water samples and sediments

[0051] Water samples: Water samples were collected in autoclaved polyethylene bottles. It is advisable to take 10cm below the water surface when collecting, and collect 3 bottles at each sampling point as 3 samples in parallel, and mark the number, collection location, and collection time. Immediately after collection, put the water sample into an incubator filled with ice cubes, and bring it back to the laboratory for processing in time.

[0052] Sediment: Sediment and sludge are collected with an autoclaved sampler and put into a sterilized 50mL centrifuge tube. Immediately after collection, the samples were placed in an incubator filled with ice cubes and brought back to the laboratory for processing in time.

[0053] Step 2: S...

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Abstract

The invention relates to a quantitative detection method of a drug resistance gene in a Taihu Lake water body and the sediment, which comprises water body and sediment sample collection, sample totalgenome DNA extraction, target DNA standard product preparation and fluorescence quantitative PCR detection, wherein, the drug resistance gene is a resistance gene sul2 in sulfonamide drug resistance bacteria and a resistance gene tetO in tetracycline drug-resistant bacteria. The method carries out absolute quantitative detection on the drug resistance gene of sulfonamides and tetracycline antibiotics in the Taihu Lake water body and the sediment through a fluorescence quantitative PCR technology, can help to know the content level and space-time distribution characteristics of tetracycline andsulfonamide resistance genes in natural water bodies and sediments such as lakes and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a quantitative detection method for drug-resistant genes in Taihu Lake water and sediments. Background technique [0002] Antibiotics are a class of secondary metabolites with anti-pathogen or other activities produced by microorganisms or higher animals and plants in the course of life, which can interfere or inhibit the growth and survival of pathogens. Common antibiotics are divided into tetracyclines, sulfonamides, aminoglycosides, β-lactams, quinolones, macrolides, chloramphenicol and polypeptides. As a drug for preventing and treating bacterial infections, antibiotics are not only widely used in clinical medicine, but also widely used in aquaculture and animal husbandry as feed additives and growth promoters. [0003] A large number of studies have shown that after the antibiotics used in the medical industry and animal husbandry are used, up to 75% of the antibiot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6851
CPCC12Q1/6851C12Q2531/113C12Q2563/107C12Q2545/114B63B34/26B63C13/00
Inventor 薛校风邢鹏周丽君
Owner NANJING NORMAL UNIVERSITY