Method and kit for simultaneously measuring 35 kinds of psychotropic drugs by efficient liquid chromatography-mass spectrometry

A technology of psychotropic drugs and LC-MS, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long drug testing time, affecting the efficiency of testing, and high cost of the testing process, and achieve optimal selection of instruments and testing conditions, Reduction of testing cost, low cost effect

Active Publication Date: 2019-04-19
杭州度安医学检验实验室有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] First, the cost is high. At present, most LC-MS/MS methods use internal standards such as deuterium to correct matrix effects and other effects. The high cost of such internal standards will directly lead to high costs for the entire detection process;
[0006] Second, the throughput is low. Existing methods mostly measure one or several drugs at the same time, but rarely involve dozens of drugs, which affects the efficiency of detection;
[0007]

Method used

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  • Method and kit for simultaneously measuring 35 kinds of psychotropic drugs by efficient liquid chromatography-mass spectrometry
  • Method and kit for simultaneously measuring 35 kinds of psychotropic drugs by efficient liquid chromatography-mass spectrometry
  • Method and kit for simultaneously measuring 35 kinds of psychotropic drugs by efficient liquid chromatography-mass spectrometry

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Embodiment 1

[0069] 1. Materials:

[0070] 1.1 The sample of methodological research experiment comes from Hangzhou Du'an Medical Laboratory;

[0071] 1.2 Instruments:

[0072] 1.2.1 Sciex 4500MD triple quadrupole mass spectrometer (USA, Sciex Company), Shimadzu HPLC system (Japan, Shimadzu Scientific Company);

[0073] 1.2.2 KQ-500E ultrasonic cleaner;

[0074] 1.2.3 H1650R desktop high-speed refrigerated centrifuge (China, Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.);

[0075] 1.2.4 G560E vortex mixer (USA, Scientific Industries company);

[0076] 1.2.5 Watsons distilled water, purchased from Guangzhou Watsons Food and Beverage Co., Ltd. (China);

[0077] 1.2.6 BT125D electronic balance (Germany, Sartorius AG);

[0078] 1.2.7 Pipettes (EPPENDORF, Germany).

[0079] 1.3 Reagent consumables:

[0080] 1.3.1 Acetonitrile, Methanol (Merck, USA)

[0081] 1.3.2 High-purity water (Wahaha)

[0082] 1.3.3 Formic acid (SIGMA Aldrich, USA)

[0083] 1.3.4 Ammonium acetate (SIGMA Aldr...

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Abstract

The invention discloses a method and kit for simultaneously measuring 35 kinds of psychotropic drugs by liquid chromatography-mass spectrometry. By optimizing chromatographic conditions and mass spectrometry conditions, the 35 kinds of psychotropic drugs can be simultaneously treated, the pretreatment step of the psychotropic drugs is simple, and protein precipitation is conducted simply through methanol. Compared with the prior art, the kit and the corresponding detection method have the advantages that the cost is greatly reduced, the analysis time is greatly shortened, the sensitivity is improved, the flux is increased, and the needs for clinical detection are met.

Description

technical field [0001] The invention relates to the field of psychotropic drug detection, in particular to a method and a kit for simultaneous determination of 35 psychotropic drugs by high performance liquid mass spectrometry. Background technique [0002] So far, more than 200 kinds of psychotropic drugs have been developed and used clinically. These psychotropic drugs have produced huge medical value and economic benefits. However, due to the problem of metabolic differences among individual patients, the therapeutic effect of these drugs on many patients is not satisfactory. Unsatisfactory, therefore, therapeutic drug monitoring (TDM) is receiving more and more attention. TDM is to titrate the dose of individual patients by quantitatively measuring the concentration of drugs in plasma or serum in order to obtain better curative effect and better tolerance. Tolerance, while also reducing the risk of poisoning. At present, among the chemical detection methods of TDM, LC-M...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 高飞海云谷云云王万强
Owner 杭州度安医学检验实验室有限公司
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