Method and kit for simultaneously measuring 35 kinds of psychotropic drugs by efficient liquid chromatography-mass spectrometry

Abstract

The invention discloses a method and kit for simultaneously measuring 35 kinds of psychotropic drugs by liquid chromatography-mass spectrometry. By optimizing chromatographic conditions and mass spectrometry conditions, the 35 kinds of psychotropic drugs can be simultaneously treated, the pretreatment step of the psychotropic drugs is simple, and protein precipitation is conducted simply through methanol. Compared with the prior art, the kit and the corresponding detection method have the advantages that the cost is greatly reduced, the analysis time is greatly shortened, the sensitivity is improved, the flux is increased, and the needs for clinical detection are met.

Description

technical field [0001] The invention relates to the field of psychotropic drug detection, in particular to a method and a kit for simultaneous determination of 35 psychotropic drugs by high performance liquid mass spectrometry. Background technique [0002] So far, more than 200 kinds of psychotropic drugs have been developed and used clinically. These psychotropic drugs have produced huge medical value and economic benefits. However, due to the problem of metabolic differences among individual patients, the therapeutic effect of these drugs on many patients is not satisfactory. Unsatisfactory, therefore, therapeutic drug monitoring (TDM) is receiving more and more attention. TDM is to titrate the dose of individual patients by quantitatively measuring the concentration of drugs in plasma or serum in order to obtain better curative effect and better tolerance. Tolerance, while also reducing the risk of poisoning. At present, among the chemical detection methods of TDM, LC-M...

AI Technical Summary

Problems solved by technology

[0005] First, the cost is high. At present, most LC-MS / MS methods use internal standards such as deuterium to correct matrix effects and other effects. The high cost of such internal standards will directly lead to high costs for the entire detection process;

[0006] Second, the throughput is low. Existing methods mostly measure one or several drugs at the same time, but rarely involve dozens of drugs, which affects the efficiency of detection;

[0007] Third, the analysis time is long. The analysis time of the LC part accounts for the vast majority of the measurement methods in the prior art, resulting in a long time for drug detection, and the general analysis time is longer than 5 minutes;

[0008] Fourth, the sensitivity is low. In the process of detecting multiple drugs at the same time, there is a problem of relatively low sensitivity of multiple targets, which affects the accuracy of detection.

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