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Ultralow-temperature culturing method for asparagus officinalis

A technology of asparagus ferns and cultivation method, which is applied in the directions of horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of high recovery rate and regeneration rate, wide variety of preservation and low cost

Inactive Publication Date: 2019-04-26
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ultra-low temperature preservation technology of Asparagus fern has not been reported at home and abroad

Method used

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  • Ultralow-temperature culturing method for asparagus officinalis

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Experimental program
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Effect test

Embodiment 1

[0029] The invention provides a method for ultra-low temperature preservation and cultivation of Asparagus dentifrice, comprising: placing the shoot tip of Asparagus dentata with a length of 1 mm cultured in a pre-culture solution in a shaker shaker and cultivating it in the dark for 1 day at room temperature, Treat with the loading solution for 20 minutes, take out the stem tip of Asparagus fern and transfer it to a vitrification solution at 0°C for dehydration for 25 minutes, then put it into a cryopreservation tube, and then store the cryopreservation tube in liquid nitrogen for ultra-low temperature preservation.

[0030] Wherein, the preculture solution is: 1 / 2MS+0.8mol / L sucrose+7g / L agar;

[0031] The loading solution is: 1 / 2MS+2mol / L glycerol+0.5mol / L sucrose

[0032] The vitrification solution is: PVS1

Embodiment 2

[0034] The invention provides a method for ultra-low temperature preservation and cultivation of Asparagus dentifrice, comprising: placing the stem tip of Asparagus dentata with a length of 2 mm cultured in a pre-culture solution in a shaker shaker for 2 days in the dark and dark for 2 days, Treat with the loading solution for 40 minutes, take out the shoot tip of Asparagus fern, transfer it to a vitrification solution at 0°C for dehydration for 40 minutes, put it into a cryopreservation tube, and then store the cryopreservation tube in liquid nitrogen for ultra-low temperature preservation.

[0035] Among them, the pre-culture solution is: MS+1.2mol / L glucose+0.2mg / mL NAA;

[0036] The loading solution is: 1 / 2MS+2mol / L glycerol+0.7mol / L sucrose

[0037] The vitrification solution is: PVS3

Embodiment 3

[0039] The invention provides a method for ultra-low temperature preservation and cultivation of Asparagus dentata, comprising: placing the stem tip of Asparagus dentata with a length of 2 mm cultured in a pre-culture solution in a shaker shaker for 3 days in the dark and dark for 3 days, Treat with the loading solution for 460 minutes, take out the stem tip of Asparagus fern, transfer it to a vitrification solution at 0°C for dehydration for 60 minutes, put it into a cryopreservation tube, and then store the cryopreservation tube in liquid nitrogen for ultra-low temperature preservation.

[0040] Among them, the pre-culture solution is: MS+1.2mol / L glucose+5%DMSO+1.0mg / mL6-BA

[0041] The loading solution is: 1 / 2MS+2mol / L glycerol+0.7mol / L sucrose

[0042] The vitrification solution is: PVS1

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Abstract

The invention discloses an ultralow-temperature culturing method for asparagus officinalis. The method comprises the steps of putting a stem tip with a length of 1mm-2mm of asparagus officinalis cultured in pre-culture liquid into a rotary table oscillator at the room temperature, carrying out light-shading dark culturing for 1-3 days, carrying out treatment for 20-60 minutes by virtue of loadingliquid, taking out the stem tip of asparagus officinalis into a vitrification solution at 0 DEG C, carrying out dehydration for 30-50 minutes, putting the stem tip into a cryopreservation tube, storing the cryopreservation tube into liquid nitrogen, and carrying out ultralow-temperature storage. According to the method, the resuscitation rate and regeneration rate of asparagus officinalis are veryhigh, the technical assurance is provided for the preservation of a severely endangered asparagus officinalis germplasm resource, and an effectively way is provided for cultivating new species by virtue of asparagus officinalis and plant diversity.

Description

technical field [0001] The invention relates to the technical field of germplasm resource preservation, in particular to an ultra-low temperature cultivation method of Asparagus fern. Background technique [0002] Asparagus officinalis is a perennial herbaceous plant of the genus Asparagus in the family Liliaceae, also known as asparagus and asparagus. According to literature reports, Asparagus japonicus is rich in minerals, amino acids, asparagus saponins, polysaccharides and flavonoids and other bioactive components, and has pharmacological activities of anti-oxidation, anti-tumor, anti-fungal and hypolipidemic. Asparagus fern can clear away heat and dampness, promote blood circulation and dissipate stagnation. It is mainly used to treat hepatitis, psoriasis, hyperlipidemia, breast hyperplasia, and has certain curative effects on lymphosarcoma, bladder cancer, breast cancer, skin cancer, etc. [0003] Cryopreservation (Cryopreservation) is a modern in vitro preservation t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01N3/00
CPCA01H4/001A01H4/005A01N3/00
Inventor 缪剑华李翠张占江韦坤华
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS