Zhongshan fir essential oil and its application in inhibiting plant pathogenic bacteria
A plant pathogenic bacteria, Zhongshan fir technology, applied in the direction of application, plant growth regulator, botany equipment and methods, etc., can solve the problems that there is no extraction of effective substances from Zhongshan fir plants, and the in-depth development and application research of Zhongshan fir is rare. , to achieve a good control effect, realize effective utilization, and reduce pollution
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Embodiment 1
[0036] The leaves of Zhongshan fir plants were collected in the Agricultural Ecological Industrial Park of Fengtai County, Anhui Province.
[0037] 3kg of fresh Zhongshan fir leaves were pulverized and placed in a volatile oil extraction device, and distilled water was added until the ratio of solid to liquid was 1:10, and mixed;
[0038] Then, add 60ml of n-hexane to the extraction device, and distill and extract at 60°C for 6h;
[0039] After cooling, separate the layers, collect the n-hexane layer, and concentrate under reduced pressure to remove the n-hexane to obtain the essential oil of Chinese fir, which is transferred to a brown sample bottle for storage at 4°C.
Embodiment 2
[0041] The leaves of Zhongshan fir plants were collected in the Agricultural Ecological Industrial Park of Fengtai County, Anhui Province.
[0042] 3kg of fresh Zhongshan fir leaves are pulverized and placed in a volatile oil extraction device, distilled water is added until the ratio of solid to liquid is 1:15, and mixed;
[0043] Then, add 100ml of n-hexane to the extraction device, and distill and extract at 60°C for 6h;
[0044] After cooling, separate the layers, collect the n-hexane layer, and concentrate under reduced pressure to remove the n-hexane to obtain the essential oil of Chinese fir, which is transferred to a brown sample bottle for storage at 4°C.
Embodiment 3
[0046] The leaves of Zhongshan fir plants were collected in the Agricultural Ecological Industrial Park of Fengtai County, Anhui Province.
[0047] 3kg of fresh Zhongshan fir leaves were pulverized and placed in a volatile oil extraction device, and distilled water was added until the ratio of solid to liquid was 1:10, and mixed;
[0048] Then, add 60ml of petroleum ether to the extraction device, and distill and extract at 60°C for 6h;
[0049] After cooling, the liquid was separated, the organic layer was collected, and the petroleum ether was removed by concentration under reduced pressure to obtain the essential oil of Chinese fir, which was transferred to a brown sample bottle and stored at 4°C.
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