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Method for degrading oily wastewater through PAM (Polyacrylamide)-SA (Sodium Alginate) immobilized microorganisms

A technology of immobilizing microorganisms, PAM-SA, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of poor anti-pollution ability, easy loss of bacterial cells, and low effective concentration.

Inactive Publication Date: 2019-04-26
NORTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at the same time, in the process of oil exploitation, transportation, storage and use, leaks are often caused by improper safety measures and sudden accidents, resulting in a large amount of oily wastewater and causing environmental pollution, so it is urgent to treat oily wastewater
[0003] In the treatment of oily wastewater, the microbial method has good prospects due to its low cost and no secondary pollution. However, in the traditional treatment process, microorganisms usually grow in a suspended state, and the effective concentration of degrading bacteria in the system is low. Loss, poor anti-pollution ability, etc.

Method used

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  • Method for degrading oily wastewater through PAM (Polyacrylamide)-SA (Sodium Alginate) immobilized microorganisms
  • Method for degrading oily wastewater through PAM (Polyacrylamide)-SA (Sodium Alginate) immobilized microorganisms
  • Method for degrading oily wastewater through PAM (Polyacrylamide)-SA (Sodium Alginate) immobilized microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Isolation and identification of embodiment 1-bacterial strain:

[0029] Inoculate the petroleum aromatics-contaminated soil suspension with 15% inoculum amount into the nutrient solution with standard oil (6g / L) as the only carbon source, and culture it on a shaker at 150 r / min under aerobic conditions at 28°C for 3-4 days. Transfer 1 time under the condition. Dilute and apply to solid medium with petroleum hydrocarbon as the only carbon source, and culture at 28°C for 4-5 days to form a single colony. Pick colonies that grow rapidly and have neat edges and put them into the nutrient solution, and shake the flask for re-screening to obtain the target strain.

[0030] The identification results at the molecular biology level are shown in the nucleotide or amino acid sequence table.

[0031] 16s rDNA phylogenetic tree analysis, figure 1 Shown is the phylogenetic tree of the BS bacteria genus of the nucleotide or amino acid sequence SEQ1.

[0032] The Neighbor-Joining M...

Embodiment 2

[0037] The preparation of embodiment 2-microbial mixed inoculum:

[0038] 1. Inoculation and activation of microbial strains

[0039] Under sterile conditions, inoculate the four strains of BS, AFS, PSS and AS into the triangular flasks containing nutrient broth (after sterilization), and put the above triangular flasks into a shaker at 30°C and 200r / min. Cultivate in the bed for 48 hours and wait for use.

[0040] 2. Fermentation preparation of liquid mixed bacterial agent:

[0041] (1) The genus Bacillus ( Bacillus encimensis strain encimensis ), Alcaligenes faecalis ( Alcaligenes faecalis strain ), Pseudomonas citutzeri ( Pseudomonas stutzeri strain ), Acinetobacter ( Acinetobacter sp ), according to the amount of 5% incorporation into bacterial fermenters for fermentation and cultivation for 24 hours, to obtain bacterial seed liquid.

[0042] (2) Preparation of mixed bacterial agent:

[0043] The above fermented bacterial seed liquid is made into a bacterial s...

Embodiment 3

[0049] Embodiment 3-PAM-SA immobilized microorganism degrades oily wastewater:

[0050] 1. Technology of polyacrylamide (PAM)-sodium alginate (SA) immobilized microorganisms to degrade oily wastewater:

[0051] In this example, the fermented microbial agent prepared in Example 3 was used to repair the oily wastewater, and 5.0% of the immobilized microbial agent was added according to the volume ratio of the wastewater to repair the oily wastewater with a concentration of 1-3%.

[0052] The specific operation is as follows:

[0053] (1) Resurrection of four microbial strains, expanded cultivation, fermentation production, and standby;

[0054] (2) The combination of polyacrylamide (PAM)-sodium alginate (SA) as the embedding method to immobilize microorganisms is represented by PSM (Polyacrylamide-Sodium Alga Acid-Microorganism). Then use the prepared solid bacterial agent to mix evenly with oily wastewater, test the oil-reducing effect of the prepared material and discuss it....

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Abstract

A method for degrading oily wastewater through a PAM (Polyacrylamide)-SA (Sodium Alginate) immobilized microorganism comprises the following steps: firstly preparing a microbial agent for efficientlydegrading the oily wastewater: screening the microbial agent from soil polluted by petroleum for a long term, taking crude oil as a sole carbon source and finally separating and screening four strainsSEQ1-BS, SEQ2-AFS, SEQ3-PSS and SEQ4-AS with efficient petroleum degradability through a screening, enriching and separating method; firstly reviving four microbial strains, then carrying out amplification culture, carrying out fermentation production, and reserving for standby use; immobilized microorganisms: establishing embedded immobilized microorganisms by taking PAM-SA as a carrier, washingby using natural saline, and reserving for standby use; detecting the degradation rate of petroleum hydrocarbon and the quantity of all components and microorganisms of the crude oil every week, andobserving the structure of spheres of the immobilized microorganism through an electron microscope at the same time until the petroleum hydrocarbon content of the oily wastewater reaches the standard.

Description

technical field [0001] The invention relates to biodegradation technology of oily wastewater. Background technique [0002] Petroleum is the main mineral resource in our country, and it is particularly important to promote the advancement and development of the economy. But at the same time, in the process of oil exploitation, transportation, storage and use, leaks are often caused by improper safety measures and sudden accidents, resulting in a large amount of oily wastewater and causing environmental pollution, so it is urgent to treat oily wastewater. [0003] In the treatment of oily wastewater, the microbial method has good prospects due to its low cost and no secondary pollution. However, in the traditional treatment process, microorganisms usually grow in a suspended state, and the effective concentration of degrading bacteria in the system is low. Loss, poor anti-pollution ability and other characteristics. Studies have shown that the key to the success of microbia...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/08C12N11/10C02F3/34C02F101/32C12R1/07C12R1/05C12R1/38
CPCC12N1/20C12N11/08C12N11/10C02F3/34C02F2101/32
Inventor 陈丽华雒晓芳孙万虹王冬梅李海玲李安杨一鸣
Owner NORTHWEST UNIVERSITY FOR NATIONALITIES
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