The sgRNA that specifically recognizes the porcine tert site, its coding DNA and its application

A specific identification and site technology, applied in the field of genetic engineering, can solve the problem that the molecular mechanism has not been fully analyzed, and achieve the effect of efficient large fragment knockout and strong specificity
CN109679958BActive Publication Date: 2022-04-26FOSHAN UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
FOSHAN UNIVERSITY
Publication Date
2022-04-26

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Abstract

The present disclosure provides sgRNA that specifically recognizes the pig Tert site, wherein the sgRNA is composed of pTert-exon1-sgRNA and pTert-exon2-sgRNA, and the nucleotide sequence responsible for recognizing the target fragment in the pTert-exon1-sgRNA is SEQ ID NO:1; the nucleotide sequence responsible for recognizing the target fragment in the pTert-exon2-sgRNA is SEQ ID NO:2. The sgRNA at the pig Tert site has strong specificity. The sgRNA at the pig Tert site can use the CRISPR / Cas9 gene editing system and the double fluorescence enrichment method, which can efficiently knock out large fragments of the pig Tert gene, and the homozygous efficiency can reach 50%. Functional research provided preliminary technical support.
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Description

technical field

[0001] The invention relates to the technical field of genetic engineering, in particular to sgRNA specifically recognizing pig Tert site, its coding DNA and application. Background technique

[0002] Aging is the biggest risk factor for human diseases, and the risks of tumors, diabetes, cardiovascular diseases, and Alzheimer's all increase year by year with age. Telomere reverse transcriptase (Tert) gene plays an important role in the process of aging, but its molecular mechanism of regulating animal aging has not been fully resolved. The physiology and other aspects of pigs are highly similar to humans, making them ideal models for human diseases. If pigs are used as the research object, the Tert gene-edited pig cell model will be prepared by genome editing technology, and the molecular mechanism of Tert gene regulating pig aging phenotype will be revealed, which will provide new ideas for the mechanism elucidation of human aging-related diseases and the d...

Claims

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