Method for simultaneously measuring various endocrine disruptors in environment water sample

A technology for endocrine disruptors and water samples, applied in the field of environmental detection, can solve the problems of single type of endocrine disruptors and low throughput of quantitative detection methods, and achieve the effects of improving analysis throughput, low cost and few steps.

Active Publication Date: 2019-04-26
NANJING UNIV
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  • Application Information

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Problems solved by technology

[0008] Aiming at the limitations of the existing quantitative detection methods for endocrine disruptors, such as low throughput and simultaneous detection of a single category of endocrine disruptors, the present invention provides a method for simultaneous determination of multiple endocrine disruptors in environmental water samples, aimed at pesticides, Herbicides, organophosphorus pesticides, organochlorine pesticides, phthalates, steroid hormones, flame retardants, pharmaceuticals and personal care products, industrial intermediates, etc., including phenols, ketones, carboxylic acids, esters , nitriles, amines and more than a hundred kinds of endocrine disruptors, and screened out a single adsorbent solid-phase extraction method with high commercialization, high quality control and low cost, using high performance liquid chromatography-triple quadrupole mass spectrometry High-throughput quantitative determination of endocrine disruptors in environmental water samples

Method used

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  • Method for simultaneously measuring various endocrine disruptors in environment water sample
  • Method for simultaneously measuring various endocrine disruptors in environment water sample
  • Method for simultaneously measuring various endocrine disruptors in environment water sample

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Experimental program
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Effect test

Embodiment 1

[0049] The list of potential endocrine disruptors released by international organizations was sorted out, the literature was searched and the compounds that could be determined by liquid chromatography-mass spectrometry (LC-MS / MS) were screened, and 103 endocrine disruptor liquid mass spectrometers as shown in Table 2 were established. Feature database, input compound information into the multiple reaction monitoring (MRM) scanning mode of high performance liquid chromatography-triple quadrupole mass spectrometry, and establish a quantitative method for target pollutants by drawing a standard curve with corresponding standard substances, compound names of endocrine disruptors, CAS number, ion summation mode, parent ion mass number, product ion mass number and collision energy in LC-MS / MS are shown in Table 2; 3 libraries are established, respectively: serial numbers 1- as shown in Table 1 Library I consisting of 36 endocrine disruptors, library II consisting of endocrine disrup...

Embodiment 2

[0084] Four environmental water samples with different pollution levels were collected in the Taihu Lake Basin, namely surface water, groundwater, source water and tap water. Each point of them collected 20L, and the following steps were used for processing:

[0085] (1) After centrifuging the water sample, take the supernatant, the pH condition is 7, and within 48 hours after sampling, use a solid phase extraction device to activate the supernatant (using n-hexane, dichloromethane, methanol, After ultrapure water activation, the flow rate is controlled at 1 drop / second) HR-X solid-phase extraction small column enrichment, the speed of water sample passing through the solid-phase extraction column is 3mL / min; the processing capacity is 1L of water sample to be tested / column, after enrichment, the extraction column is centrifuged or vacuum-dried;

[0086] (2) Use 6 mL / column of methanol, dichloromethane and n-hexane to elute the extraction column in step (1) successively, and ...

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Abstract

The invention discloses a method for simultaneously measuring various endocrine disruptors in an environment water sample, and belongs to the field of environment detection. The method includes the steps of firstly, taking a water sample, taking supernate after conducting natural depositing or centrifuging, enriching the supernate through an activated solid-phase extraction small column by means of a solid-phase extraction device, and conducting centrifuging or vacuum drying on the extraction column after enriching; secondly, eluting the extraction column through an organic solvent, and conducting volume fixing through methyl alcohol after eluant is concentrated to prepare to-be-measured liquid; thirdly, establishing a quantifying method of target pollutants; fourthly, measuring the concentration of the endocrine disruptors in the water sample through the efficient liquid-phase chromatography-triple quadrupole mass spectrometry. The method is small in reagent consumption, low in cost and few in step, but can simultaneously quantitatively analyze the endocrine disruptors in the water body, several types to more than one dozen of types of traditional concerned endocrine disruptors are expanded into 103 types of endocrine disruptors, the analysis flux is greatly improved, the application range is expanded, and the potential high-risk endocrine disruptors in the environment water sample can be better recognized.

Description

technical field [0001] The invention belongs to the field of environmental detection, and more specifically relates to a method for simultaneously measuring multiple endocrine disruptors in environmental water samples. Background technique [0002] Endocrine Disrupting Chemicals (EDCs), also known as Environmental Hormone, are hormones or compounds that can affect the endocrine disrupting system of humans or wild animals. They enter organisms through ingestion, accumulation and other ways, but they do not directly bring abnormal effects on organisms as toxic substances, but act similar to hormones on organisms. Even at extremely low concentrations, they can make organisms The endocrine imbalance of human body causes various abnormal effects. Such substances can interfere with the endocrine regulation function of organisms at low concentrations, and have adverse effects on reproductive and developmental functions, so they have attracted much attention. Studies have shown th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72Y02A20/20
Inventor 史薇周成卓沈艳红林叠于红霞
Owner NANJING UNIV
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