Method for preparing double target magnetic nanoparticle of liver cancer

A magnetic nanoparticle, dual-targeting technology, applied in the field of nanomaterial manufacturing, can solve the problems of suboptimal, low surgical resection rate, low survival rate, etc., and achieve the effect of great clinical application prospects.

Inactive Publication Date: 2019-05-03
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although surgical treatment has achieved significant curative effect, however, because HCC is mostly found in the middle and late stages, the surgical resection rate is low, and the long-term prognosis of advanced patients is not good even if liver transplantation is performed.
A variety of minimally invasive interventional methods, including transarterial chemoembolization (TACE) and radiofrequency ablation (FRA), have been widely used in clinical practice, but the 5-year survival rate is still low

Method used

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  • Method for preparing double target magnetic nanoparticle of liver cancer
  • Method for preparing double target magnetic nanoparticle of liver cancer
  • Method for preparing double target magnetic nanoparticle of liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Preparation of liver cancer dual targeting magnetic nanoparticles

[0037] 1. Preparation of superparamagnetic nanoparticles (SPIO) (high temperature pyrolysis method):

[0038] Add 2mmol of iron acetylacetonate (Fe(acac)3) into a 200mL inclined three-neck flask, continue to add 20mL of dibenzyl ether, and then add oleic acid (6mmol) and oleylamine (6mmol) successively. Under the protection of nitrogen atmosphere, the mixture was heated to 200° C., and reacted at this temperature for 60 minutes, and then heated to 280° C., and reacted at this temperature for 30 minutes. After interrupting heating and cooling to room temperature, add 40ml of ethanol for magnetic separation and washing, repeat several times to remove residual dibenzyl ether, oleic acid and oleylamine in the system, and finally prepare oleic acid-modified magnetic Fe 3 o 4 Nanoparticles, which were stored in n-hexane solution, and its scanning electron microscope pictures can be found in figu...

Embodiment 2

[0043] Example 2: MR analysis of different concentrations of PEGylated magnetic nanoparticles cross-linked double antibody and PEGylated magnetic nanoparticles, the preparation method is the same as that of Example 1.

[0044] The concentrations of Fe were 0.00625, 0.0125, 0.025, 0.05, and 0.1 mM, respectively. The result is as Figure 7 shown. From Figure 7 It can be seen that as the concentration of SPIO-PEG and SPIO-PEG-BsAb increased, the T2 signal of both decreased significantly, indicating that the sensitivity of MR imaging was not reduced after SPIO-PEG was linked with double antibodies.

[0045] MR analysis (T2 value) of different concentrations of PEGylated magnetic nanoparticles Figure 8 ,From Figure 8 It can be seen that as the concentration increases, the T2 signal decreases significantly, showing a linear relationship. The linear analysis graph of MR analysis (T2 value) after different concentrations of PEGylated magnetic nanoparticles linked to antibodies...

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Abstract

The invention discloses a method for preparing double target magnetic nanoparticle of liver cancer. The preparation method of the invention comprises preparation of superparamagnetic nanoparticles (SPIO), PEG modification of the superparamagnetic nanoparticles (SPIO), and modification of SPIO cross-linking double anti-CD34 and anti-AFP by using DSPE-PEG2000. The nanoparticle prepared by the invention can target the alpha-fetoprotein-positive liver cancer cells and the liver cancer neovascular endothelial cells, and the magnetic resonance MR imaging can dynamically trace the SPIO-PEG-BsAb in vivo, and the important role of evaluating the diagnosis of liver cancer is evaluated from the molecular level. The method realizes the purpose of dual molecular imaging of liver cancer cells and livercancer blood vessels, and provides new ideas and new ways for the molecular level diagnosis of liver cancer and other malignant tumors and the dynamic evaluation of anti-angiogenic targeted therapy.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial manufacturing, in particular to a method for preparing liver cancer dual-targeting magnetic nanoparticles. Background technique [0002] Hepatocellular carcinoma (HCC) is the fifth most common malignant tumor in the world and the third most common malignant tumor in my country. Although surgical treatment has achieved remarkable curative effect, however, because HCC is mostly found in the middle and advanced stages, the surgical resection rate is low, and the long-term prognosis of advanced patients is not good even if liver transplantation is performed. A variety of interventional minimally invasive treatment methods including transarterial chemoembolization (TACE) and radiofrequency ablation (Radio Frequency Ablation, FRA) have been widely used in clinic, but the 5-year survival rate is still low. [0003] Superparamagnetic iron oxide (SPIO) nanoparticles are ideal magnetic resonance trace...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K49/12A61K49/16
Inventor 孙军辉周官辉艾静张岳林周坦洋吴李鸣朱统寅陈新华周民任志刚
Owner ZHEJIANG UNIV
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