Microfluidic chip for accurately controlling and pairing single particles and applications thereof
A microfluidic chip and microparticle technology, which is applied in specific-purpose bioreactors/fermenters, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the difficulties in the isolation and pairing process and the lack of cell types Selectivity, it is difficult to achieve multi-cell pairing, etc., to overcome the Poisson distribution, easy to operate, and cost-effective
A microfluidic chip and microparticle technology, which is applied in specific-purpose bioreactors/fermenters, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the difficulties in the isolation and pairing process and the lack of cell types Selectivity, it is difficult to achieve multi-cell pairing, etc., to overcome the Poisson distribution, easy to operate, and cost-effective
CN109722385AActive Publication Date: 2019-05-07WISDOM HEALTHY BIOTECH XIAMEN CO LTD
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Embodiment 1
[0064] like Figure 7 As shown, the present invention is used for precise control and pairing of single-particle chips, including three parts: channel layer A, control layer B and slide C, wherein channel layer A is located at the uppermost layer, control layer B is located in the middle, and the slide sheet is located at the bottom. lower level.
[0065] Wherein the channel layer A includes a plurality of single particle capture and transfer units, in this embodiment, these units are connected in series (such as Figure 4 and Figure 5 shown). In other embodiments, these units may also be connected in parallel or in a hybrid manner.
[0066] see Figure 1 to Figure 6 , each unit includes a capture channel 1 , a capture chamber 2 , a capture slit 3 , a transfer channel 4 , a matching chamber 5 and a matching slit 6 . exist figure 1 In , the flow direction of capture channel 1 is from left to right.
[0067] The capture chamber 2 is located in the capture flow channel 1,...
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Abstract
The invention discloses a microfluidic chip for accurately controlling and pairing single particles. The chip comprises a channel layer and a control layer. The channel layer comprises a plurality ofunits for capturing and transferring single particles, and each unit consists of a capture flow channel, a capture chamber, a capture gap, a transfer flow channel, a pairing chamber and a pairing gap.The control layer is positioned below the capture flow channel and a pairing flow channel, is vertical to the capture flow channel and the pairing flow channel, and is isolated by a diaphragm. The chip can efficiently and accurately control the capture and transfer of single particles, and can realize high-throughput and high-efficiency single-particle pairing after different rounds of single-particle capture and transfer, and the number and the type of the paired particles are controllable. The chip can be widely applied to single-cell isolation and culture, single-cell heterogeneity analysis, multi-cell co-culture, multi-cell interaction, potential mechanism disclosure and the like.
Description
technical field [0001] The invention relates to a microfluidic chip for precise manipulation and pairing of single particles, which can be applied to the isolation and cultivation of single cells, the analysis of single cell heterogeneity, the co-cultivation of multiple cells, the revelation of multi-cell interactions and potential mechanisms, etc. . Background technique [0002] Due to the advantages of miniaturization, integration, high throughput, and precise control of microfluidics, microfluidic technology has been more and more widely used in the fields of physics, chemistry, biology, and engineering science. One of the important applications is to realize Precise manipulation and pairing of single particles. Taking a single cell as an example, a cell is the basic unit of the structure and function of an organism, and the function of a multicellular organism is inseparable from the interaction between single cells. Therefore, a comprehensive and accurate understandin...
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Patent Timeline
07 May 2019
Publication
CN109722385A
- IPC
- C12M3/00; C12M1/36
- Inventors
- 杨朝勇; 刘卫枝