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Culture medium capable of promoting spore production by bacillus subtilis and application thereof

A Bacillus subtilis culture medium technology, applied in the field of microbial engineering, can solve the problem of low spore production of Bacillus subtilis, achieve the effects of reducing elimination rate, improving digestibility, and promoting spore production

Active Publication Date: 2019-05-07
COFCO BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a culture medium capable of promoting spore production of Bacillus subtilis and its application in order to overcome the low number of spores produced by Bacillus subtilis in the prior art

Method used

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  • Culture medium capable of promoting spore production by bacillus subtilis and application thereof
  • Culture medium capable of promoting spore production by bacillus subtilis and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0022] The method for preparing animal feed (such as poultry feed, especially broiler feed) provided by the present invention comprises carrying out liquid culture to Bacillus subtilis according to the above method, spray-drying the culture solution obtained, and mixing the dried product obtained with basic nutrients .

[0023] According to the invention, the spray drying is generally carried out in a spray dryer. The inlet temperature of the spray dryer can be 150-200°C, and the outlet temperature can be 100-120°C. The duration of spray drying can be 10-60s. The carrier used for spray drying can be various commonly used carriers, generally a carrier that does not affect the nutrition and taste of animal feed, preferably at least one of corn flour, corn bran, corn cob and water-soluble starch. The weight ratio of the carrier used in spray drying to the material to be dried (culture solution) is (0.5-1.5):1.

[0024] According to a preferred embodiment of the present inventi...

Embodiment 1

[0029] (1) according to the culture medium and condition of table 1, use the flask of 250ml to carry out first-level shake flask and secondary shake flask (activation) to Bacillus subtilis, then, use the expansion tank that effective volume is 400L to expand Bacillus subtilis train.

[0030] Table 1

[0031]

[0032]

[0033] (2) Then the nutrient solution in the expansion tank (the characterization results such as germination rate are shown in Table 1) is spray-dried, and the conditions are as follows:

[0034] Spray carrier: corncob, the weight ratio to the culture medium is 1:1

[0035] Inlet temperature: 150°C

[0036] Outlet temperature: 100°C

[0037] Duration: 10s

[0038] (3) Add the dry matter obtained in step (2) to the ration of broilers, and add 5×10 per gram of ration. 14 The amount of bacteria in CFU was added to obtain Feed 1.

Embodiment 2

[0040]The feed is prepared according to the method of Example 1, the difference is that 10 14 The amount of the bacteria of CFU was added to the dry matter, and Feed 2 was obtained.

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Abstract

The present invention relates to the field of microbial engineering and discloses a culture medium capable of promoting spore production by bacillus subtilis and an application thereof. The provided culture medium comprises 0.3-0.8% by weight of yeast powder, 0.3-0.8% by weight of corn syrup, 0.3-0.8% by weight of molasses, 1.5-2.5% by weight of corn flour, 2-3% by weight of soybean meal and 0.008-0.02% by weight of manganese sulfate, and pH is 7-7.5. The present invention also provides a use of the above culture medium for culturing the bacillus subtilis or for preparing animal feeds. The present invention also provides a method for preparing the animal feeds. The method comprises steps of using the culture medium to conduct liquid culture of the bacillus subtilis, conducting spray dryingof obtained culture liquid, and mixing obtained dry matter with basic nutrients. The culture medium can effectively promote the spore production by the bacillus subtilis.

Description

technical field [0001] The invention relates to the field of microbial engineering, in particular to a culture medium capable of promoting spore production of Bacillus subtilis and an application thereof. Background technique [0002] With the popularization of the application range of fermented feed, it is imminent to increase the output of fermented feed, and the preparation of liquid seed liquid is a key factor restricting the production; the preparation process of liquid seed liquid is complicated and the storage period is short; therefore, solid bacterial agents are used in fermented feed, It is a breakthrough development that can solve the limitations of equipment, technology, manpower, etc., and is an important factor for stabilizing and improving product quality and increasing production. [0003] Bacillus subtilis can synthesize digestive enzymes by itself, such as protease, amylase, lipase, etc.; during the fermentation and storage of the whole diet, the enzymes pr...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/125
Inventor 佟毅张德国俞建良卢宗梅陈影吴大利张琳孙晶刘梦涵刘利利
Owner COFCO BIOTECHNOLOGY CO LTD
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