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A medium capable of promoting spore production of Bacillus subtilis and its application

A Bacillus subtilis culture medium technology, applied in the field of microbial engineering, can solve the problem of low number of spores produced by Bacillus subtilis, achieve the effects of reducing elimination rate, improving digestibility, and promoting spore production

Active Publication Date: 2020-01-03
COFCO BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a culture medium capable of promoting spore production of Bacillus subtilis and its application in order to overcome the low number of spores produced by Bacillus subtilis in the prior art

Method used

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  • A medium capable of promoting spore production of Bacillus subtilis and its application
  • A medium capable of promoting spore production of Bacillus subtilis and its application

Examples

Experimental program
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Effect test

preparation example Construction

[0022] The method for preparing animal feed (such as poultry feed, especially broiler feed) provided by the present invention includes liquid culture of Bacillus subtilis according to the above method, spray drying of the obtained culture solution, and mixing of the obtained dried product with basic nutrients .

[0023] According to the present invention, the spray drying is generally performed in a spray dryer. The inlet temperature of the spray dryer can be 150-200°C, and the outlet temperature can be 100-120°C. The duration of spray drying can be 10-60s. The carrier used in spray drying can be various commonly used carriers, generally those that do not affect the nutrition and taste of animal feed, preferably at least one of corn flour, corn husk, corn cob and water-soluble starch. The weight ratio of the carrier used in spray drying to the material to be dried (culture solution) is (0.5-1.5):1.

[0024] According to a preferred embodiment of the present invention, relative t...

Embodiment 1

[0029] (1) According to the medium and conditions in Table 1, use a 250ml flask to perform primary shake flask and secondary shake flask (activation) on Bacillus subtilis, and then use an expansion tank with an effective volume of 400L to expand Bacillus subtilis Training.

[0030] Table 1

[0031]

[0032]

[0033] (2) Then spray-dry the culture solution in the expansion tank (see Table 1 for the characterization results such as germination rate) under the following conditions:

[0034] Spray carrier: corncob, the weight ratio of culture solution is 1:1

[0035] Inlet temperature: 150℃

[0036] Outlet temperature: 100℃

[0037] Duration: 10s

[0038] (3) Add the dried product obtained in step (2) to the diet of broilers, and add 5×10 per gram of diet. 14 The amount of CFU bacteria was added to obtain feed 1.

Embodiment 2

[0040] The feed was prepared according to the method of Example 1, except that 10 per gram of diet was added 14 The amount of CFU bacteria was added with the dried material to obtain feed 2.

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Abstract

The present invention relates to the field of microbial engineering and discloses a culture medium capable of promoting spore production by bacillus subtilis and an application thereof. The provided culture medium comprises 0.3-0.8% by weight of yeast powder, 0.3-0.8% by weight of corn syrup, 0.3-0.8% by weight of molasses, 1.5-2.5% by weight of corn flour, 2-3% by weight of soybean meal and 0.008-0.02% by weight of manganese sulfate, and pH is 7-7.5. The present invention also provides a use of the above culture medium for culturing the bacillus subtilis or for preparing animal feeds. The present invention also provides a method for preparing the animal feeds. The method comprises steps of using the culture medium to conduct liquid culture of the bacillus subtilis, conducting spray dryingof obtained culture liquid, and mixing obtained dry matter with basic nutrients. The culture medium can effectively promote the spore production by the bacillus subtilis.

Description

Technical field [0001] The invention relates to the field of microbial engineering, in particular to a culture medium capable of promoting spore production of Bacillus subtilis and its application. Background technique [0002] With the popularization of the application range of fermented feed, the improvement of fermented feed yield is imminent, and the preparation of liquid seed liquid is a key factor restricting the yield; the preparation process of liquid seed liquid is complicated and the storage period is short; therefore, solid inoculants are used in fermented feed. It is a breakthrough development that can solve the limitations of equipment, technology, manpower and other directions, and is an important factor for stabilizing and improving product quality and increasing output. [0003] Bacillus subtilis bacteria can synthesize digestive enzymes, such as protease, amylase, lipase, etc.; during the fermentation and storage of the whole diet, the enzyme system produced can de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/125
Inventor 佟毅张德国俞建良卢宗梅陈影吴大利张琳孙晶刘梦涵刘利利
Owner COFCO BIOTECHNOLOGY CO LTD
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