Method for positioning rice complex trait gene
A technology of gene positioning and rice, applied in the direction of genomics, biochemical equipment and methods, microbial measurement/inspection, etc., to achieve the effect of low cost, high efficiency and short time
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[0035] Example 1
[0036] A method for gene mapping of complex traits in rice includes the following steps:
[0037] (1) Population construction: Select Donglan ink with black brown rice seed coat and anthocyanin content as high as 1642.99 μg / g, and Huang Huazhan with white brown rice seed coat and anthocyanin as low as 3.84 μg / g as parents, using Huang Huazhan Cross with Donglan Momi and self-cross to obtain F 2 group;
[0038] (2) Phenotypic identification and grouping: the parents of step (1) Huang Huazhan, Donglan Momi and F 2 The color of the seed coat of each individual plant in the population was determined by high performance liquid chromatography to determine the anthocyanin content of brown rice. According to the seed coat color and anthocyanin content of each individual plant, the F 2 The group is divided into 4 subgroups: completely black, partly black, brown, and white;
[0039] The above-mentioned brown rice anthocyanins include 6 kinds of anthocyanins: geranium pigment,...
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