Application of porphyra polysaccharide in improving drought-resisting property of plant

A technology of laver polysaccharides and plants, applied in the application field of laver polysaccharides in improving the drought resistance of plants, can solve the problems of inability to achieve comprehensive drought resistance and single effect, and achieve normal growth and development, improve comprehensive resistance, and ensure water content Effect

Active Publication Date: 2019-05-17
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the effect of "abscisic acid" is single, and it...

Method used

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  • Application of porphyra polysaccharide in improving drought-resisting property of plant
  • Application of porphyra polysaccharide in improving drought-resisting property of plant
  • Application of porphyra polysaccharide in improving drought-resisting property of plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 A kind of preparation method of laver polysaccharide

[0045] Mix 100 g of dried laver powder with 4 L of distilled water, extract at 100° C. for 4 h, and filter with gauze silk and diatomaceous earth to obtain the supernatant. The filtrate was dialyzed for 48 hours, concentrated by rotary evaporation to 1 / 4 of the original volume, added 4 times the volume of ethanol to the concentrated solution, precipitated overnight at 4°C, centrifuged to obtain a precipitate, and obtained a white powder product by freeze-drying, namely laver polysaccharide, with a molecular weight of 370.5 kDa, the laver polysaccharide is named PP.

Embodiment 2

[0046] Embodiment 2 A kind of preparation method of low molecular weight laver polysaccharide

[0047]The laver polysaccharide in Example 1 was dissolved in distilled water, and the laver polysaccharide accounted for 2% by mass of water, and concentrated hydrochloric acid was added so that the final concentration of hydrochloric acid was 0.1mol / L, and the volume percentage of hydrogen peroxide was 0.3%. The microwave power was set at 600W, the reaction temperature was set at 70°C, and the degradation was carried out in a microwave reactor for 10 minutes to obtain a low molecular weight laver polysaccharide with a molecular weight of 29.0kDa. The laver polysaccharide was named DPP1.

Embodiment 3

[0048] Embodiment 3 A kind of preparation method of low molecular weight laver polysaccharide

[0049] The laver polysaccharide in Example 1 was dissolved in distilled water, and the laver polysaccharide accounted for 2% by mass of water, and concentrated hydrochloric acid was added so that the final concentration of hydrochloric acid was 0.1mol / L, and the volume percentage of hydrogen peroxide was 0.3%. The microwave power was set at 600W, the reaction temperature was set at 70°C, and the degradation was carried out in a microwave reactor for 5 minutes to obtain a low-molecular-weight laver polysaccharide with a molecular weight of 48.8kDa. The laver polysaccharide was named DPP2.

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Abstract

The invention provides application of porphyra polysaccharide in improving drought-resisting property of a plant and belongs to the technical field of plant growth regulation. According to the application, the porphyra polysaccharide can regulate the osmotic pressure of plant cells under drought stress, so that the leaf water content is guaranteed. The porphyra polysaccharide also can improve theactivity of antioxidase in the plant and alleviate peroxidation of a plasma membrane caused by drought stress, so that the comprehensive resistance of the plant to drought stress is further improved,and normal growth and development of the plant are guaranteed. The application adopts laver as a raw material, the sources are rich, the cost is low and environmental protection is achieved.

Description

technical field [0001] The invention relates to the technical field of plant growth regulators, in particular to the application of porphyra polysaccharides in improving the drought-resistant performance of plants. Background technique [0002] Drought is one of the main environmental stresses in agricultural production, which seriously affects the growth and yield of crops, and its loss to crops ranks first among all abiotic stresses. Wheat is the second largest food crop in my country, and its yield has been affected by drought stress. Therefore, improving the drought resistance of crops has important practical significance and application value to ensure high and stable crop yields, national food security and sustainable production. [0003] "Abscisic acid" is one of the most important plant hormones, which widely exists in plants and participates in the regulation of various stages of plant growth and development. In the case of water shortage, the level of "abscisic a...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01G13/00C08B37/00A01N43/16A01P21/00
Inventor 邹平鲁雪莉袁源张成省李义强
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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