Functional beverage containing birch mushroom extract
A functional and extractive technology, applied in food science and other directions, can solve the problem that development and utilization are not widely popularized, and achieve the effects of improving immunity, improving sleep quality, and preventing bacterial invasion.
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[0031] The present invention is prepared according to the following method, detailed as follows:
[0032] ①Raw material preparation: Crush Inonotus obliquus (chaga velvet) into 1-2cm-sized shapes without cleaning; the dried whole piece of Gallus pilosulae, wash twice under running water, and set aside; slice and dry boletus and wash under running water Second time, spare.
[0033] Nano-preparation for extraction: The tap water is processed through double-layer nano-filtration equipment.
[0034] ② Put the Inonotus obliquus and the treated nano-water into the extraction tank at a ratio of 1:30 (mass ratio) of solid to liquid, and extract for the first time at a temperature of 100°C for 3 hours; , the supernatant is vacuum pumped into the concentration tank for standby; the filter residue continues to be extracted for the second time.
[0035] ③ The Inonotus obliquus filter residue after the first extraction is extracted for the second time with nano water at a ratio of 1:30 (...
experiment example 1
[0058] Experimental example 1 Comparison of DPPH and scavenging ability
[0059] Accurately weigh 4mg of DPPH dissolved in absolute ethanol and dilute to 100mL to obtain 100 umol / L DPPH working solution; on the basis of the pre-experiment, dilute the sample solution by 1 times, 10 times and 50 times respectively; the sample solution Add 3mL of DPPH working solution to 1mL, shake well, keep it in the dark for 30min, and then measure the absorbance at a wavelength of 517nm as Ai; add 3mL of DPPH working solution to 1mL of distilled water instead of sample solution, and measure the absorbance as A0; add distilled aqueous solution to 1mL of sample solution 3mL measured absorbance as Aj. The clearance rate was calculated by the following formula. Take Vc as the control.
[0060]
[0061] DPPH·is a stable free radical in organic solvents, its color is purple, and it has strong absorption at 517nm wavelength. When there is a free radical scavenging substance in the system, it w...
experiment example 2
[0062] Experimental example 2 Comparison of ABTS+ removal ability
[0063] Prepare 7mmol / L ABTS+ and 2.45mmol / L potassium persulfate solution, mix equal volumes and place in the dark for 16 hours to prepare ABTS stock solution; dilute the stock solution with absolute ethanol to the absorbance at 734nm wavelength before use 2.00±0.10 ABTS+ working fluid. On the basis of the pre-experiment, the sample solution was diluted 1 times, 10 times, and 50 times respectively, and 3 mL of ABTS+ working solution and 0.3 mL of sample solution were taken. After reacting at room temperature for 6 minutes, they were quickly measured at a wavelength of 734 nm, and the measured absorbance was A sample; In the same way, take 3mL of ABTS+ working solution and 0.3mL of distilled water, and measure the absorbance as A blank; take 3mL of distilled water and 0.3mL of sample solution to measure the absorbance as A control, and calculate the clearance rate by the following formula.
[0064]
[0065]...
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