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Selective enrichment method for dicaffeylspermine type compound in fructus lycii

A technology of caffeine and caffeine essence, which is applied in the field of selective enrichment of dicaffeamides in Lycium barbarum, and can solve the problems of time-consuming, low efficiency and poor selectivity

Active Publication Date: 2019-05-28
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the similar hydrophobicity of dicaffeoylspermide compounds and non-dicaffeoylspermide impurities in the sample, the conventional reversed-phase method is inefficient, time-consuming, and selective. poor

Method used

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  • Selective enrichment method for dicaffeylspermine type compound in fructus lycii
  • Selective enrichment method for dicaffeylspermine type compound in fructus lycii

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Embodiment 1

[0023] 1) Use 90% (v / v) ethanol-water solution as the extraction solvent (solid-to-liquid ratio: 1:10) for 5 g of Lycium barbarum, ultrasonically extract for 3 hours, suction filter, and centrifuge to obtain Lycium barbarum extract.

[0024] 2) A strong cation-exchange solid-phase extraction column was used to selectively enrich the crude component of dicaffeoylspermidine for the extract of wolfberry fruit. The solid-phase extraction conditions: the solid-phase extraction column was a strong cation solid-phase extraction column (1g / 6mL , 60 μm); sample volume: 4ml (Fruit medlar extract); washing conditions: washing 1: 90% (v / v) methanol-water solution 5mL; washing 2: 30% (v / v) acetonitrile -Water 5mL, rinse 2 solution with 1.0M sodium dihydrogen phosphate to adjust the pH value to 2.5, collect the eluate, concentrate under reduced pressure until the organic solvent is completely removed, and obtain the crude fraction of dicaffeamide.

[0025] 3) Desalting the crude fraction of...

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Abstract

The invention discloses a selective enrichment method for a dicaffeylspermine type compound in fructus lycii. Specifically, the selective enrichment method comprises the following steps: eluting the dicaffeylspermine type compound under a low pH (Potential of Hydrogen) condition to ensure the stability of the compound; carrying out a strong cation exchange solid-phase extraction method on fructuslycii ethanol extract and separating to obtain a dicaffeylspermine coarse component; then carrying out inversed-phase and solid-phase extraction column desalting on the obtained dicaffeylspermine typecomponent and preparing a fine component. According to the method disclosed by the invention, selective enrichment of the dicaffeylspermine type compound in a complicated system can be realized, theproblem that the compound and impurities are co-eluted on an inversed-phase material is solved and classified separation of the compound is realized. Therefore, the invention establishes the selectiveenrichment method for the dicaffeylspermine type compound and technical supports are provided for new drug research and development of the compound.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a selective enrichment method for dicaffeoylspermidine compounds in Lycium barbarum, in particular to a selective enrichment method using a strong cation exchange solid-phase extraction column under low pH conditions. A new method for the enrichment of dicaffeoylspermidine compounds, which shows a better separation effect on the enrichment of dicaffeoylspermide compounds in complex systems. Background technique [0002] Dicaffeoylspermidine is a plant secondary metabolite present in Solanaceae plants. Dicaffeoylspermide belongs to amides. According to literature reports, this type of chemical composition has various pharmacological activities such as scavenging free radicals in the body, anti-aging, neuroprotection, anti-tumor, anti-cancer, anti-inflammation, and prevention of Alzheimer's disease. (Li Y Y, Di R, Baibado J T, etal. Identification of kukoamines as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C231/24C07C235/34B01D15/18B01D15/36B01D15/32
Inventor 梁鑫淼艾散江·艾海提金红利刘艳芳郭志谋
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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