41results about How to "Achieve selective enrichment" patented technology

The invention relates to a mono-dispersed core-shell structure polymer nano particle, preparation and applications thereof. At first, monoene functional monomers and polyene crosslinking monomers are polymerized to generate polymer particles taken as the cores of the polymer microsphere, and then 3-acrylamino phenylboronic acid (APBA) and polyene crosslinking monomers are covered on the surface of the cores through a reversible addition-fragmentation chain transfer (RAFT) polymerization technology so as to obtain smooth core-shell polymer nano particles containing boronic acid functional groups. Phenylboronic acid functional groups are introduced to the material surface through a RAFT method, thus the defects existing in the conventional post modification method such as tedious steps, low reaction efficiency, and unstable morphology of direct polymerized products are avoided, moreover, the APBA can be more stably polymerized, and the bonding amount of boronic acid on the surface is increased at the same time. The polymer nano particle can be used to separate or enrich glycoprotein in a 1,2-cis-diol structure, and has a very good practical value and application prospect in fields like proteomics, etc.

The invention discloses a micro-extraction monolithic column of an ionic liquid polymer capillary tube and a preparation method thereof. The method comprises the following steps: by adopting imidazolium ionic liquids and dodecyl methacrylate as functional monomers, taking ethylene glycol dimethyl acrylic ester as a cross-linking agent, taking normal propyl alcohol and butanediol as pore-foaming agents and taking azodiisobutyronitrile as an initiator, evenly mixing the raw materials at room temperature in an oscillating manner, and injecting into a vinyl-modified quartz capillary tube; sealing, and carrying out thermal initiation one-step polymerization, so as to obtain the micro-extraction monolithic column of the ionic liquid polymer capillary tube in manners of cleaning and drying. The micro-extraction monolithic column is simple and convenient in synthetic process, controllable in reaction, and low in cost. The prepared micro-extraction column for the capillary tube has good permeability, porosity, and chemical and mechanical stability; a plurality of interaction sites such as an imidazole ring with positive electricity and different lengths of carbon chains and the like are formed on the surface; the micro-extraction monolithic column has good extraction ability on the substances such as phenolic environmental hormone and the like, and is quick to extract, and easy to build an analysis method with high sensitivity by combining with capillary electrochromatography.

The invention relates to the field of material analysis chemistry and organic chemistry. The invention provides a phosphopeptide enriching material and a preparation method and application thereof. The phosphopeptide enriching material comprises a substrate and a double-ingredient copolymer layer formed on the surface of the substrate, wherein the thickness of the double-ingredient copolymer layer is 10 to 80nm. The surface initiation-atom transfer free radical polyreaction mechanism is utilized; the double-ingredient copolymers are grafted on the surface of the substrate. The material and column solid phase extraction mode or dispersion solid phase extraction mode are organically combined; the enriching with high selectivity, high repetitiveness and high flux of phosphopeptide in a complicated mixture can be realized, so that the selective enriching of the multi-phosphopeptide and mono-phosphopeptide can be realized; the phosphorylated protein identification number can be obviously improved. Therefore the phosphopeptide enriching material is hopeful to be widely applied to the aspects of phosphopeptide enriching, further large-scale separation of phosphorylated protein and the like.

The invention provides a preparation method of an embedded attapulgite capillary monolithic column. The method comprises the following steps of mixing acidified attapulgite and an organic polymer solution at a mass ratio being 1:100 to 5:100; performing magnetic stirring for 1 to 5 hours at the normal temperature so that the acidified attapulgite is completely dispersed; then, injecting the material into a quartz capillary treated by gamma-2-methyl propyl trimethoxy silane; sealing the material; then putting the materials into a constant-temperature water bath pot for reaction for 16 to 24h at 60 to 85 DEG C; after the reaction is completed, using acetonitrile and water for flushing; preparing the material for use. The natural attapulgite nanometer materials are embedded into an organic polymer monolithic material for the first time; the embedded attapulgite capillary monolithic column is prepared. The preparation method has the advantages that the simplicity is realized; the cost is low; the reaction condition can be easily controlled; the prepared embedded attapulgite capillary monolithic column has higher mechanical intensity and higher permeability; when the prepared embedded attapulgite capillary monolithic column is used as an adsorbing agent to be applied to solid micro extraction, the advantages of great adsorption volume, high recovery yield, good reproducibility, low detection limit and the like are realized. The preparation method is applicable to the sample pretreatment of polar small-molecular compounds.

The invention discloses a method for enriching glycopeptide and simultaneously enriching glycopeptide and phosphorylated peptide by using a metal oxide. The method is characterized in that: the metal oxide is used as a solid-phase extraction material for separating and enriching the glycopeptide from protein enzymolysis solution, and simultaneous enrichment of the phosphorylated peptide and the glycopeptide can be realized in a sample by using simple operation. Specifically, the method comprises the following steps of: mixing the metal oxide and the sample under a neutral or weak acid condition, eluting the mixture under an acid condition to obtain the glycopeptide, and eluting the mixture under an alkali condition to obtain the phosphorylated peptide. The method can realize high-efficiency and high-selectivity enrichment of the phosphorylated peptide and the glycopeptide in the same sample.

The invention relates to a modified mesoporous silica adsorbent and a preparation method and application thereof. Under the catalysis of potassium carbonate and potassium iodide, aza-macrocyclic ligand is modified onto the surface of chlorine group functional mesoporous silica through nucleophilic substitution, and the adsorbent is prepared; palladium recycling and enrichment under different nitric acid concentrations are achieved through the adsorbent, and the selective recovery performance of palladium in highly-active effluent simulated feed liquid is evaluated. According to the modified mesoporous silica adsorbent, mesoporous nanomaterials are combined with the supermolecule recognition performance of the aza-macrocyclic ligand, the problems that selectivity is poor, operation is complex, the raw material cost is high and the stability is poor in the traditional treatment technology for palladium in highly-active effluent are solved, and the adsorbent has the advantages of being good in selectivity, large in adsorption capacity, high in adsorption speed and easy to recycle for palladium within the wide nitric acid concentration range, and has the great practical significance in the aspects of nuclear fuel reprocessing and hydrometallurgy.

The invention belongs to the technical field of preparation technology for adsorption and enrichment materials and detection methods for bisphenol compounds, and relates to a preparation method and application of a high-efficiency adsorption material for bisphenol compounds. The preparation method comprises: fully mixing the mixed functional monomer and the hydrophilic organic framework material in an organic solvent, adding a cross-linking agent and an initiator, deoxidizing, and heating to react to obtain the bisphenol compound adsorbent. composite material. The composite material prepared by the invention has stable chemical properties, good hydrophilicity, high porosity and strong adsorption performance for bisphenol chemicals. The cost of raw materials used in the present invention is low, the material preparation process is simple, the adsorption rate is fast, the adsorption capacity is high, and it is convenient for recycling. As an efficient adsorption material, it can be used for the rapid enrichment and removal of bisphenol compounds in the water environment, and can also be used The extraction agent in the pretreatment process is combined with instrument analysis to establish a highly sensitive detection method for bisphenols.

The invention relates to the field of material analysis chemistry and organic chemistry. The invention provides a phosphopeptide enriching material and a preparation method and application thereof. The phosphopeptide enriching material comprises a substrate and a double-ingredient copolymer layer formed on the surface of the substrate, wherein the thickness of the double-ingredient copolymer layer is 10 to 80nm. The surface initiation-atom transfer free radical polyreaction mechanism is utilized; the double-ingredient copolymers are grafted on the surface of the substrate. The material and column solid phase extraction mode or dispersion solid phase extraction mode are organically combined; the enriching with high selectivity, high repetitiveness and high flux of phosphopeptide in a complicated mixture can be realized, so that the selective enriching of the multi-phosphopeptide and mono-phosphopeptide can be realized; the phosphorylated protein identification number can be obviously improved. Therefore the phosphopeptide enriching material is hopeful to be widely applied to the aspects of phosphopeptide enriching, further large-scale separation of phosphorylated protein and the like.

The invention relates to the preparation and application of a guanidine-based immobilized affinity magnetic sphere with a core-shell structure, which adopts superparamagnetic iron tetroxide microspheres as a substrate, and 3-(trimethoxysilyl)methanol is bonded on the surface Vinyl-modified magnetic spheres were formed from acrylate-based acrylates, and polymer-coated magnetic spheres with a core-shell structure were obtained by distillation-precipitation polymerization; guanidinium-immobilized core-shell structures were obtained by functional modification of the polymer layer. Polymer coated magnetic balls. In the preparation material of the present invention, utilizing the strong interaction between the guanidinium group and the phosphate group, the polyphosphopeptides in the α-casein (α-casein) or β-casein (α-casein) enzymolysis solution can be respectively realized Or the selective enrichment of all phosphopeptides, the enrichment capacity for phosphopeptides reaches 200 mg / g, the detection limit for phosphopeptides reaches 0.5 fmol, and the recovery rate for phosphopeptides enrichment reaches 91.30%. Utilizing the superparamagnetism of the material, rapid and complete separation can be achieved within 30s.

The invention discloses a fluorescent probe based on a lanthanide ion hybrid covalent organic framework material as well as a preparation method and application thereof. The lanthanide ion hybrid covalent organic framework material is prepared from a carboxylated imine type covalent organic framework and europium chloride hexahydrate through a coordination reaction; according to the lanthanide ion hybrid covalent organic framework material disclosed by the invention, not only is the strong affinity of a covalent organic framework to target molecules extracted and enriched, the sensitivity is improved, but also carboxylate radicals are coordinated with lanthanide ions, a new fluorescence center is formed, and environmental interference is reduced. The constructed lanthanide ion hybrid covalent organic framework material can be used for constructing a high-performance fluorescent probe which is high in sensitivity, high in selectivity and high in stability and is used for detecting tetracycline.

The invention provides a method for analyzing sulfonamide substances by magnetic solid-phase extraction and isotope dimethyl mark. The method includes the steps: taking magnetic carbon nano-particles as extraction materials; dispersing the materials in sample solution with sulfanilamide; enriching sulfonamide compounds in samples to the surfaces of the magnetic carbon nano-particles by the aid of a solid-phase extraction method; performing dimethyl mark for sulfonamide compound normal positions adsorbing on the surfaces of the materials; eluting and collecting marked samples; analyzing the eluted and collected samples by the aid of an LC-MS (liquid chromatography-mass spectrometry) to obtain results. The new method has the advantages that trace sulfonamide compounds in water are quantitatively analyzed on magnetic nano-spheres by a dimethyl mark method and the LC-MS, the method is simple and convenient to operate, rapid in analyzing, and trace sulfonamide substances in environmental media can be accurately and quantitatively analyzed.

The invention discloses an application method of a hydrophobic extractant in a cloud point extraction system. The extractant is carried into the cloud point extraction system by forming an O / W microemulsion, which skillfully solves the problem of poor water solubility of the extractant. The problem of being unable to solubilize in the system has successfully realized the selective enrichment of target ions by the cloud point extraction system. The invention realizes the application of hydrophobic extractant in the cloud point extraction system for the first time, and the cloud point extraction technology can still selectively enrich the target actinide ions under the interference of many lanthanide ions, which is efficient and fast, and avoids a large amount of organic waste The production of liquid reduces environmental pollution, simplifies the post-processing process, and reduces post-processing costs. In addition, the combined use of ICP-MS can also realize the rapid and accurate detection of trace target ions, which can form a universal separation and analysis method.

The invention provides a method for analyzing sulfonamide substances by magnetic solid-phase extraction and isotope dimethyl mark. The method includes the steps: taking magnetic carbon nano-particles as extraction materials; dispersing the materials in sample solution with sulfanilamide; enriching sulfonamide compounds in samples to the surfaces of the magnetic carbon nano-particles by the aid of a solid-phase extraction method; performing dimethyl mark for sulfonamide compound normal positions adsorbing on the surfaces of the materials; eluting and collecting marked samples; analyzing the eluted and collected samples by the aid of an LC-MS (liquid chromatography-mass spectrometry) to obtain results. The new method has the advantages that trace sulfonamide compounds in water are quantitatively analyzed on magnetic nano-spheres by a dimethyl mark method and the LC-MS, the method is simple and convenient to operate, rapid in analyzing, and trace sulfonamide substances in environmental media can be accurately and quantitatively analyzed.

The invention relates to a polymer nanoparticle with monodisperse core-shell structure and its preparation and application. The polymer particles prepared by polymerization of monoene functional monomers and polyene crosslinking monomers are used as the core, and then 3-acrylamidophenylboronic acid is coated on the surface of the core by reversible addition-fragmentation chain transfer (RAFT) polymerization technology (APBA) and polyene cross-linked monomers to form polymer nanoparticles with a smooth surface and a core-shell structure with boric acid functional groups. The present invention introduces phenylboronic acid functional groups on the surface of the material through the RAFT method, which not only overcomes the shortcomings of traditional post-modification methods such as cumbersome steps, low reaction efficiency, and unstable product morphology during direct polymerization, but also enables APBA to polymerize more stably and improve the surface Amount of boric acid bonded. The polymer nanoparticle of the present invention can be used for separating or enriching glycoproteins with 1,2-cis-diol structure, and has good practical value and application prospect in the fields of proteomics and the like.

The invention discloses a glycopeptide enrichment method based on amphipathic porous nuclear shell microspheres. The method includes the steps of firstly, conducting activating with an eluant 1 and balancing with a sample loading solution 1 on large-pore nuclear shell microspheres in sequence by means of a dispersed solid-phase extraction mode, dissolving a sample in the sample loading solution 1,mixing the solution with the balanced large-pore nuclear shell microspheres, conducting incubating and centrifuging to obtain supernate 1, and spin-drying the supernate 1 to obtain powder 1; secondly, conducting flushing with an eluant 2 and balancing with a sample loading solution 2 on the large-pore nuclear shell microspheres in sequence by means of a dispersed solid-phase extraction mode, dissolving the powder obtained through spin-drying in the first step in the sample loading solution 2, mixing the solution with the balanced small-pore nuclear shell microspheres, conducting incubating and centrifuging, abandoning supernate to obtain precipitates 2, flushing the precipitates 2 with leacheate 2, conducting centrifuging again after oscillating, abandoning supernate to obtain small-porenuclear shell microspheres adsorbing glycopeptides, eluting the glycopeptides adsorbed on the small-pore nuclear shell microspheres with the eluant 2, and concentrating the glycopeptides. The method is widely suitable for concentrating a trace amount of glycopeptides in a large amount of protein and non-glycopeptide samples.

The invention discloses a selective enrichment method for dicaffeoylspermide compounds in Lycium barbarum. Specifically, the dicaffeoylspermidine compounds are eluted under low pH conditions to ensure that such compounds The stability of the compound, the ethanol extract of Lycium barbarum was separated by strong cation exchange solid phase extraction to obtain the crude dicaffeoylspermide component, and then the obtained dicaffeoylspermide component was subjected to reversed-phase solid-phase extraction. Extraction column is desalted to prepare refined components; this method can realize the selective enrichment of dicaffeoylspermidine compounds in complex systems, solve the problem of "co-elution" of such compounds and impurities on reversed-phase materials, and realize Class separation of this class of compounds. Therefore, the present invention establishes a new method for the selective enrichment of dicaffeoylspermide compounds, providing technical support for the research and development of new drugs of such compounds.

The invention belongs to the technical field of high molecular materials and analysis and relates to a monodisperse core-shell structure polymer nano particle as well as preparation and application thereof. The polymer nano particle is a core-shell type copolymer of an acrylic acid cross-linking agent and a monoene phenylboronic acid functional monomer; and the polymer nano particle is prepared by using a one-pot method, that is, precipitation polymerization with the combination of in-situ package strategy. Synthesis steps of the conventional core-shell structure polymer particles are simplified, and the polymer nano particle has the characteristics of rapidness, convenience and easiness, low cost, multiple boric acid functional monomers on the surface, and the like. The polymer nano particle can be used for separating or enriching glycoprotein with a 1,2-cis-glycol structure, and has good practical values and application prospects in the fields of proteomics and the like.

The present invention relates to the fields of material analysis chemistry, post-translational modification proteomics and the like, and provides a method for separating and enriching glycopeptides. According to the method, a highly nitrogen-doped carbon material having a two-dimensional porous crystal structure contacts a proteolysate, the hydrophilic interaction, the electrostatic interaction, CH3-pi and other actions between the material and glycopeptides are used, glycopeptides are separated and enriched by using a solid-phase extraction (SPE) or dispersive solid-phase extraction (dSPE) separation mode, and the sample is analyzed through mass spectrometry. According to the present invention, by optimizing the compositions, the pH values, the buffer salts and other parameters of the sample solution, the leaching solution and the eluent, the selective enrichment of glycopeptides is achieved; and the method has advantages of good stability, high selectivity, large adsorption capacity,strong flexibility, easy and controllable operation and the like, and can be used for the glycopeptide enrichment of standard products and complex samples.