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Method for electrochemical detection of antibiotics in milk based on vertical and ordered micelle enrichment

A vertically ordered micelles and vertically ordered technology, applied in the field of electrochemical sensing, can solve the problems of low detection accuracy and easy to be affected by external adjustments, achieve high sensitivity, wide detection linear range, and expand application space Effect

Active Publication Date: 2016-01-13
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above-mentioned methods are to determine whether antibiotics are contained by detecting indirect parameters, the detection accuracy is low, and they are easily affected by external adjustments

Method used

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  • Method for electrochemical detection of antibiotics in milk based on vertical and ordered micelle enrichment
  • Method for electrochemical detection of antibiotics in milk based on vertical and ordered micelle enrichment
  • Method for electrochemical detection of antibiotics in milk based on vertical and ordered micelle enrichment

Examples

Experimental program
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Effect test

Embodiment 1

[0035] The electrochemical process of chloramphenicol in milk was detected using vertically ordered CTAB micelles modified ITO electrode:

[0036] (1) With 0.1mol / L, the sodium chloride aqueous solution that pH value is 6 will dilute the milk that direct supermarket buys 10 times, as the solution to be tested, as figure 1 shown. Argon gas was passed through the solution to be tested to eliminate the interference of oxygen in the solution. Using a simple three-electrode system, such as Figure 7 As shown, vertical ordered CTAB modified ITO electrode, platinum electrode and silver / silver chloride electrode were used as working electrode, counter electrode and reference electrode, respectively.

[0037] (2) chloramphenicol is dissolved in dehydrated alcohol and is configured into stock solution, and the chloramphenicol stock solution of adding different volumes obtains a series of solutions to be tested (the concentration of chloramphenicol is respectively 0.4ppm, 0.7ppm in the...

Embodiment 2

[0048] The electrochemical process of nitrofurantoin in milk was detected using vertically ordered CTAB micelles modified ITO electrode:

[0049] (1) Dilute the milk purchased directly from the supermarket by 5 times with a 0.1mol / L phosphate buffer solution with a pH value of 7, and use it as the solution to be tested. Nitrogen gas is passed through the solution to be tested to eliminate the interference of oxygen in the solution. A simple three-electrode system was adopted, and the vertically ordered CTAB-modified FTO electrode, platinum electrode, and silver / silver chloride electrode were used as the working electrode, counter electrode, and reference electrode, respectively.

[0050] (2) Dissolve nitrofurantoin in absolute ethanol to prepare a stock solution, add different volumes of nitrofurantoin stock solution to the test solution to obtain a series of test solutions, and perform differential pulse voltammetry after enrichment at -0.4V potential for 30s test.

[0051]...

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Abstract

The present invention discloses a method for electrochemical detection of antibiotics in milk based on vertical and ordered micelle enrichment. The method is characterized by comprising: diluting milk with an electrolyte solution so as to be adopted as a detected solution, adopting vertical and ordered CTAB micelle modified electrode as a working electrode, using an electrochemical voltammetry method, enriching antibiotics in the milk solution, and then detecting. According to the present invention, the vertical and ordered micelle modified electrode is adopted as the working electrode, the hydrophobic and electrically neutral antibiotics in the aqueous solution are extracted / enriched into the hydrophobic nanometer chambers of the surfactant micelles through the hydrophobic effect, and the reduction peak current of the antibiotics is adopted as the detection signal so as to achieve the quantitative detection in the complex milk background; and the constructed sensor integrated the pre-enrichment and the electrochemical detection, has characteristics of high sensitivity, wide linear detection range and low detection limit, and has great application potential in the food safety monitoring field.

Description

technical field [0001] The invention belongs to the field of electrochemical sensing, in particular to a method for detecting antibiotics in milk. Background technique [0002] Food safety in the current society has attracted widespread attention, among which dairy product safety is a major focus. Exploring fast, accurate, convenient and sensitive analytical methods has become one of the key directions of dairy product quality and safety research. Antibiotics are a class of substances that can inhibit the growth of bacteria or kill bacteria, and are widely used as animal drugs and feed additives. Studies have shown that antibiotic residues in milk have serious harm to the human body, such as allergic reactions, drug-resistant strains, and bacterial imbalance in the human intestinal tract. At present, countries such as the United States, the European Union, and Japan have made clear regulations on the maximum residue of antibiotics in raw milk, and the maximum residue is 4 μ...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/327
Inventor 苏彬晏菲姚丽娜郑雯静
Owner ZHEJIANG UNIV
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