Ultrasonic composite acidic water extraction method for cordyceps polysaccharide and cordycepin in cordyceps militaris

An extraction method and ultrasonic technology, applied in the field of ultrasonic compound acid water extraction, can solve the problems of long time consumption, large ethanol volume, low polysaccharide purity, etc., and achieve the effects of avoiding introduction and residue, increasing dissolution, and increasing destructive effect.

Active Publication Date: 2019-05-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention consumes a huge amount of ethanol and only uses boiling water bath to extract it, which takes a long time and has low efficiency. The content of the extracted product is high except for the polysaccharide of Cordyceps polysaccharide, and the purity of the polysaccharide is relatively low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Using dilute hydrochloric acid solution as the extractant combined with ultra-low temperature freeze-thaw and low-frequency ultrasonic technology to assist in the extraction of Cordyceps polysaccharides and cordycepin from the fruiting bodies of Cordyceps militaris

[0045]The fruiting body of Cordyceps militaris is picked, washed, dried, pulverized, and degreased to obtain dry powder of Cordyceps militaris leftovers, and then it is immersed in a prepared dilute hydrochloric acid solution (pH=4.8), and frozen at ultra-low temperature (-80°C)-microwave ( 400W, 2min) thawing cycle 3 times, at the same time using low-frequency ultrasonic (frequency 20-30KHz, power 350W) assisted, stirring and extracting at 65°C for 40min, and then rotating the extract at 60°C and 0.095MPa to concentrate to 1% of the original volume, the collected concentrate was frozen at -70°C for 24 hours, and finally it was freeze-dried to collect the dry powder of the water-extracted product....

Embodiment 2

[0048] Example 2: Extracting Cordyceps polysaccharide and cordycepin in the fruiting body of Cordyceps militaris by using citric acid solution as the extractant combined with ultra-low temperature freeze-thaw and low-frequency ultrasonic technology

[0049] The fruiting body of Cordyceps militaris is picked, cleaned, dried, pulverized, and degreased to obtain the dry powder of Cordyceps militaris leftovers, and then it is immersed in the prepared citric acid solution (pH=4.8), and ultra-low temperature freezing (-80°C)-microwave ( 400W, 2min) thawing cycle 3 times, at the same time using low-frequency ultrasonic (frequency 20-30KHz, power 350W) assisted, stirring and extracting at 65°C for 40min, and then rotating the extract at 60°C and 0.095MPa to concentrate to 1% of the original volume, the collected concentrate was frozen at -70°C for 24 hours, and finally it was freeze-dried to collect the dry powder of the water-extracted product. The final extraction yields of Cordycep...

Embodiment 3

[0052] Example 3: Extracting Cordyceps polysaccharide and cordycepin in the fruiting body of Cordyceps militaris by using acetic acid solution as the extractant combined with ultra-low temperature freeze-thaw and low-frequency ultrasonic technology

[0053] The fruiting body of Cordyceps militaris is picked, washed, dried, crushed, and degreased to obtain the dry powder of Cordyceps militaris leftovers, and then it is immersed in the prepared acetic acid solution (pH=4.9), and ultra-low temperature freezing (-80°C)-microwave (300W , 3min) thawing cycle 3 times, at the same time using low-frequency ultrasonic (frequency 20-30KHz, power 350W) to assist, stirring and extracting at 65°C for 40min, and then rotating the extract at 60°C and 0.095MPa to concentrate to 1% of the original volume, the collected concentrate was frozen at -70°C for 24 hours, and finally it was freeze-dried to collect the dry powder of the water-extracted product. The final extraction yields of Cordyceps p...

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PUM

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Abstract

The invention provides an ultrasonic composite acidic water extraction method for cordyceps polysaccharide and cordycepin in cordyceps militaris, and belongs to the technical field of food processing.The ultrasonic composite acidic water extraction method comprises the steps that cordyceps militaris fruit bodies are picked, washed, dried, pulverized and degreased to obtain dry powder of the cordyceps militaris, then the dry powder of the cordyceps militaris is immersed in a prepared dilute hydrochloric acid solution, ultra-low temperature freezing and microwave defrosting are repeated for 3 times and low-frequency ultrasonic waves are used for assisted stirring and extraction at the same time, an extracting solution is subjected to evaporation concentration treatment, and finally, dry powder of a water extraction product is collected by freeze drying of the extracting solution. The dosage of an additive used in the ultrasonic composite acidic water extraction method meets the use requirements of food additives at home and abroad, the operation is simple and easy to perform, the combination of chemical and physical methods is adopted, and time saving and efficiency are achieved.

Description

technical field [0001] The invention relates to an ultrasonic composite acidic water extraction method of cordyceps polysaccharide and cordycepin in Cordyceps militaris, belonging to the technical field of food processing. Background technique [0002] Cordyceps militaris (CM) is a traditional edible and medicinal mushroom in China, commonly known as Cordyceps militaris, which belongs to Ascomycota and Pyrethroidaceae fungi, and has been proved to have similar pharmacological effects to Cordyceps sinensis. Cordyceps militaris has been used for hundreds of years in many Asian countries, especially China, and it is rich in special natural ingredients with biological and pharmacological activities, such as cordycepin (3′-deoxyadenosine), adenosine, cordycepic acid, SOD enzyme and Cordyceps polysaccharide. Traditional Chinese medicine believes that the water extract of Chinese medicine has the functions of nourishing the lungs and kidneys, relieving waist and knee pain, and hel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07H19/16C07H1/08
CPCC07H19/16C07H1/08B01D11/0288B01D11/0265B01D11/0211C08B37/0003C08L5/00A23L31/00A01G18/70C08B37/00
Inventor 张慜石浩易思富郭小玲侯丽华阳武雄
Owner JIANGNAN UNIV
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